Amfizemli Hastalarda α1-Proteinaz İnhibitörü Aktivitesi ve Fenotiplerinin Belirlenmesi

In our study, functionally active α1-proteinase inhibitor (α1-PI) levels and phenotypeswere determined in sera of patients with emphysema and in that of healthy subjects. Patients were classified according to their smoking habits: Group I consistedof 7 nonsmoking (or limited history of smoking) pati...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Özden Tacal, Nilgün Sümer, Lütfi Çöplü
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2006
Materias:	Emphysema α1-proteinase inhibitor phenotypes cigarette smoking isoelectric focusing. Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/80bffa75b357436186044104b61b59eb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:80bffa75b357436186044104b61b59eb
record_format	dspace
spelling	oai:doaj.org-article:80bffa75b357436186044104b61b59eb2021-12-02T13:04:31ZAmfizemli Hastalarda α1-Proteinaz İnhibitörü Aktivitesi ve Fenotiplerinin Belirlenmesi0250-46851303-829Xhttps://doaj.org/article/80bffa75b357436186044104b61b59eb2006-12-01T00:00:00Zhttp://www.turkjbiochem.com/2006/169.pdfhttps://doaj.org/toc/0250-4685https://doaj.org/toc/1303-829XIn our study, functionally active α1-proteinase inhibitor (α1-PI) levels and phenotypeswere determined in sera of patients with emphysema and in that of healthy subjects. Patients were classified according to their smoking habits: Group I consistedof 7 nonsmoking (or limited history of smoking) patients. Group II consisted of heavily smoking 14 patients. Four patients having intermediate smoking habits were considered independently (Group III). Two of these patients were siblings with a history of familial emphysema. The Control group consisted of 28 healthy nonsmokingsubjects. Active serum α1-PI levels were assayed spectrophotometrically, by determining the extent of trypsin inhibition. α1-PI phenotypes were determined by isoelectric focusing on polyacrylamide gels. Once the serum α1-PI levels have been compared, the differences between Group I and Group II and the Control group were statistically insignificant (p >0.05). Isoelectric focusing results showed that all but 4 patients had the M1M1 or M1M2 phenotype. (The siblings in the Group III had the ZZ phenotype and 2 patients in Group II had undefined phenotypes). Considering these results, we concluded that, with the exception the siblings expressingthe ZZ variant, the development of emphysema in our patients was caused by an increase in elastase load rather than a deficiency in antielastase capacity. The increase in elastase load was most likely due to biological and chemical oxidants in the lungsÖzden Tacal,Nilgün SümerLütfi ÇöplüDe GruyterarticleEmphysemaα1-proteinase inhibitorphenotypescigarette smokingisoelectric focusing.BiochemistryQD415-436ENTürk Biyokimya Dergisi, Vol 31, Iss 4, Pp 169-174 (2006)
institution	DOAJ
collection	DOAJ
language	EN
topic	Emphysema α1-proteinase inhibitor phenotypes cigarette smoking isoelectric focusing. Biochemistry QD415-436
spellingShingle	Emphysema α1-proteinase inhibitor phenotypes cigarette smoking isoelectric focusing. Biochemistry QD415-436 Özden Tacal, Nilgün Sümer Lütfi Çöplü Amfizemli Hastalarda α1-Proteinaz İnhibitörü Aktivitesi ve Fenotiplerinin Belirlenmesi
description	In our study, functionally active α1-proteinase inhibitor (α1-PI) levels and phenotypeswere determined in sera of patients with emphysema and in that of healthy subjects. Patients were classified according to their smoking habits: Group I consistedof 7 nonsmoking (or limited history of smoking) patients. Group II consisted of heavily smoking 14 patients. Four patients having intermediate smoking habits were considered independently (Group III). Two of these patients were siblings with a history of familial emphysema. The Control group consisted of 28 healthy nonsmokingsubjects. Active serum α1-PI levels were assayed spectrophotometrically, by determining the extent of trypsin inhibition. α1-PI phenotypes were determined by isoelectric focusing on polyacrylamide gels. Once the serum α1-PI levels have been compared, the differences between Group I and Group II and the Control group were statistically insignificant (p >0.05). Isoelectric focusing results showed that all but 4 patients had the M1M1 or M1M2 phenotype. (The siblings in the Group III had the ZZ phenotype and 2 patients in Group II had undefined phenotypes). Considering these results, we concluded that, with the exception the siblings expressingthe ZZ variant, the development of emphysema in our patients was caused by an increase in elastase load rather than a deficiency in antielastase capacity. The increase in elastase load was most likely due to biological and chemical oxidants in the lungs
format	article
author	Özden Tacal, Nilgün Sümer Lütfi Çöplü
author_facet	Özden Tacal, Nilgün Sümer Lütfi Çöplü
author_sort	Özden Tacal,
title	Amfizemli Hastalarda α1-Proteinaz İnhibitörü Aktivitesi ve Fenotiplerinin Belirlenmesi
title_short	Amfizemli Hastalarda α1-Proteinaz İnhibitörü Aktivitesi ve Fenotiplerinin Belirlenmesi
title_full	Amfizemli Hastalarda α1-Proteinaz İnhibitörü Aktivitesi ve Fenotiplerinin Belirlenmesi
title_fullStr	Amfizemli Hastalarda α1-Proteinaz İnhibitörü Aktivitesi ve Fenotiplerinin Belirlenmesi
title_full_unstemmed	Amfizemli Hastalarda α1-Proteinaz İnhibitörü Aktivitesi ve Fenotiplerinin Belirlenmesi
title_sort	amfizemli hastalarda α1-proteinaz i̇nhibitörü aktivitesi ve fenotiplerinin belirlenmesi
publisher	De Gruyter
publishDate	2006
url	https://doaj.org/article/80bffa75b357436186044104b61b59eb
work_keys_str_mv	AT ozdentacal amfizemlihastalardaa1proteinazinhibitoruaktivitesivefenotiplerininbelirlenmesi AT nilgunsumer amfizemlihastalardaa1proteinazinhibitoruaktivitesivefenotiplerininbelirlenmesi AT lutficoplu amfizemlihastalardaa1proteinazinhibitoruaktivitesivefenotiplerininbelirlenmesi
_version_	1718393514545381376

Amfizemli Hastalarda α1-Proteinaz İnhibitörü Aktivitesi ve Fenotiplerinin Belirlenmesi

Ejemplares similares