Independent pathways can transduce the life-cycle differentiation signal in Trypanosoma brucei.

African trypanosomes cause disease in humans and livestock, generating significant health and welfare problems throughout sub-Saharan Africa. When ingested in a tsetse fly bloodmeal, trypanosomes must detect their new environment and initiate the developmental responses that ensure transmission. The...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Balazs Szöőr, Naomi A Dyer, Irene Ruberto, Alvaro Acosta-Serrano, Keith R Matthews
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/80c2c4c388114e0ca043abd07886144f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:80c2c4c388114e0ca043abd07886144f
record_format	dspace
spelling	oai:doaj.org-article:80c2c4c388114e0ca043abd07886144f2021-11-18T06:07:27ZIndependent pathways can transduce the life-cycle differentiation signal in Trypanosoma brucei.1553-73661553-737410.1371/journal.ppat.1003689https://doaj.org/article/80c2c4c388114e0ca043abd07886144f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24146622/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374African trypanosomes cause disease in humans and livestock, generating significant health and welfare problems throughout sub-Saharan Africa. When ingested in a tsetse fly bloodmeal, trypanosomes must detect their new environment and initiate the developmental responses that ensure transmission. The best-established environmental signal is citrate/cis aconitate (CCA), this being transmitted through a protein phosphorylation cascade involving two phosphatases: one that inhibits differentiation (TbPTP1) and one that activates differentiation (TbPIP39). Other cues have been also proposed (mild acid, trypsin exposure, glucose depletion) but their physiological relevance and relationship to TbPTP1/TbPIP39 signalling is unknown. Here we demonstrate that mild acid and CCA operate through TbPIP39 phosphorylation, whereas trypsin attack of the parasite surface uses an alternative pathway that is dispensable in tsetse flies. Surprisingly, glucose depletion is not an important signal. Mechanistic analysis through biophysical methods suggests that citrate promotes differentiation by causing TbPTP1 and TbPIP39 to interact.Balazs SzöőrNaomi A DyerIrene RubertoAlvaro Acosta-SerranoKeith R MatthewsPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 10, p e1003689 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Balazs Szöőr Naomi A Dyer Irene Ruberto Alvaro Acosta-Serrano Keith R Matthews Independent pathways can transduce the life-cycle differentiation signal in Trypanosoma brucei.
description	African trypanosomes cause disease in humans and livestock, generating significant health and welfare problems throughout sub-Saharan Africa. When ingested in a tsetse fly bloodmeal, trypanosomes must detect their new environment and initiate the developmental responses that ensure transmission. The best-established environmental signal is citrate/cis aconitate (CCA), this being transmitted through a protein phosphorylation cascade involving two phosphatases: one that inhibits differentiation (TbPTP1) and one that activates differentiation (TbPIP39). Other cues have been also proposed (mild acid, trypsin exposure, glucose depletion) but their physiological relevance and relationship to TbPTP1/TbPIP39 signalling is unknown. Here we demonstrate that mild acid and CCA operate through TbPIP39 phosphorylation, whereas trypsin attack of the parasite surface uses an alternative pathway that is dispensable in tsetse flies. Surprisingly, glucose depletion is not an important signal. Mechanistic analysis through biophysical methods suggests that citrate promotes differentiation by causing TbPTP1 and TbPIP39 to interact.
format	article
author	Balazs Szöőr Naomi A Dyer Irene Ruberto Alvaro Acosta-Serrano Keith R Matthews
author_facet	Balazs Szöőr Naomi A Dyer Irene Ruberto Alvaro Acosta-Serrano Keith R Matthews
author_sort	Balazs Szöőr
title	Independent pathways can transduce the life-cycle differentiation signal in Trypanosoma brucei.
title_short	Independent pathways can transduce the life-cycle differentiation signal in Trypanosoma brucei.
title_full	Independent pathways can transduce the life-cycle differentiation signal in Trypanosoma brucei.
title_fullStr	Independent pathways can transduce the life-cycle differentiation signal in Trypanosoma brucei.
title_full_unstemmed	Independent pathways can transduce the life-cycle differentiation signal in Trypanosoma brucei.
title_sort	independent pathways can transduce the life-cycle differentiation signal in trypanosoma brucei.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/80c2c4c388114e0ca043abd07886144f
work_keys_str_mv	AT balazsszoor independentpathwayscantransducethelifecycledifferentiationsignalintrypanosomabrucei AT naomiadyer independentpathwayscantransducethelifecycledifferentiationsignalintrypanosomabrucei AT ireneruberto independentpathwayscantransducethelifecycledifferentiationsignalintrypanosomabrucei AT alvaroacostaserrano independentpathwayscantransducethelifecycledifferentiationsignalintrypanosomabrucei AT keithrmatthews independentpathwayscantransducethelifecycledifferentiationsignalintrypanosomabrucei
_version_	1718424550443581440

Independent pathways can transduce the life-cycle differentiation signal in Trypanosoma brucei.

Ejemplares similares