Comparative Genomic Study of <named-content content-type="genus-species">Lactobacillus jensenii</named-content> and the Newly Defined <named-content content-type="genus-species">Lactobacillus mulieris</named-content> Species Identifies Species-Specific Functionality

ABSTRACT Lactobacilli are dominant members of the “healthy” female urogenital microbiota. One of these species, Lactobacillus jensenii, is routinely identified in the urinary microbiota of women both with and without urinary tract symptoms. In March 2020, the new bacterial species Lactobacillus muli...

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Autores principales:	Catherine Putonti, Jason W. Shapiro, Adriana Ene, Oleksandra Tsibere, Alan J. Wolfe
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Lactobacillus Lactobacillus jensenii Lactobacillus mulieris urinary microbiome urogenital microbiome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/80c3593f88e74079833541c4d403eaee
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spelling	oai:doaj.org-article:80c3593f88e74079833541c4d403eaee2021-11-15T15:30:51ZComparative Genomic Study of <named-content content-type="genus-species">Lactobacillus jensenii</named-content> and the Newly Defined <named-content content-type="genus-species">Lactobacillus mulieris</named-content> Species Identifies Species-Specific Functionality10.1128/mSphere.00560-202379-5042https://doaj.org/article/80c3593f88e74079833541c4d403eaee2020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00560-20https://doaj.org/toc/2379-5042ABSTRACT Lactobacilli are dominant members of the “healthy” female urogenital microbiota. One of these species, Lactobacillus jensenii, is routinely identified in the urinary microbiota of women both with and without urinary tract symptoms. In March 2020, the new bacterial species Lactobacillus mulieris was introduced, and phylogenetic and average nucleotide identity analysis identified eight L. jensenii strains that should be classified as members of the L. mulieris species. This prompted our phylogenomic study of all publicly available L. jensenii and L. mulieris genome sequences. While there is little variation in the 16S rRNA gene sequences, the core genome shows a clear distinction between genomes of the two species. We find eight additional strains of the species L. mulieris among these genomes. Furthermore, one strain, currently classified as L. mulieris UMB7784, is distinct from both L. jensenii and L. mulieris strains. As part of our comparative genomic study, we also investigated the genetic content that distinguishes these two species. Unique to the L. jensenii genomes are several genes related to catabolism of disaccharides. In contrast, L. mulieris genomes encode several cell surface and secreted proteins that are not found within the L. jensenii genomes. These L. jensenii-specific and L. mulieris-specific loci provide insight into phenotypic differences of these two species. IMPORTANCE Lactobacillus species play a key role in the health of the urinary tract. For instance, Lactobacillus crispatus and L. jensenii have been found to inhibit uropathogenic Escherichia coli growth. While L. crispatus is typically found only within the microbiota of women without lower urinary tract symptoms (LUTS), L. jensenii has been found in the microbiota of women both with and without LUTS. With the recent introduction of the new species Lactobacillus mulieris, several strains of L. jensenii were reclassified as L. mulieris based upon gene marker and average nucleotide identity. We took a phylogenomic and comparative genomic approach to ascertain the genetic determinants of these two species. Looking at a larger data set, we identified additional L. mulieris strains, including one distinct from other members of the species—L. mulieris UMB7784. Furthermore, we identified unique loci in each species that may have clinical implications.Catherine PutontiJason W. ShapiroAdriana EneOleksandra TsibereAlan J. WolfeAmerican Society for MicrobiologyarticleLactobacillusLactobacillus jenseniiLactobacillus mulierisurinary microbiomeurogenital microbiomeMicrobiologyQR1-502ENmSphere, Vol 5, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lactobacillus Lactobacillus jensenii Lactobacillus mulieris urinary microbiome urogenital microbiome Microbiology QR1-502
spellingShingle	Lactobacillus Lactobacillus jensenii Lactobacillus mulieris urinary microbiome urogenital microbiome Microbiology QR1-502 Catherine Putonti Jason W. Shapiro Adriana Ene Oleksandra Tsibere Alan J. Wolfe Comparative Genomic Study of <named-content content-type="genus-species">Lactobacillus jensenii</named-content> and the Newly Defined <named-content content-type="genus-species">Lactobacillus mulieris</named-content> Species Identifies Species-Specific Functionality
description	ABSTRACT Lactobacilli are dominant members of the “healthy” female urogenital microbiota. One of these species, Lactobacillus jensenii, is routinely identified in the urinary microbiota of women both with and without urinary tract symptoms. In March 2020, the new bacterial species Lactobacillus mulieris was introduced, and phylogenetic and average nucleotide identity analysis identified eight L. jensenii strains that should be classified as members of the L. mulieris species. This prompted our phylogenomic study of all publicly available L. jensenii and L. mulieris genome sequences. While there is little variation in the 16S rRNA gene sequences, the core genome shows a clear distinction between genomes of the two species. We find eight additional strains of the species L. mulieris among these genomes. Furthermore, one strain, currently classified as L. mulieris UMB7784, is distinct from both L. jensenii and L. mulieris strains. As part of our comparative genomic study, we also investigated the genetic content that distinguishes these two species. Unique to the L. jensenii genomes are several genes related to catabolism of disaccharides. In contrast, L. mulieris genomes encode several cell surface and secreted proteins that are not found within the L. jensenii genomes. These L. jensenii-specific and L. mulieris-specific loci provide insight into phenotypic differences of these two species. IMPORTANCE Lactobacillus species play a key role in the health of the urinary tract. For instance, Lactobacillus crispatus and L. jensenii have been found to inhibit uropathogenic Escherichia coli growth. While L. crispatus is typically found only within the microbiota of women without lower urinary tract symptoms (LUTS), L. jensenii has been found in the microbiota of women both with and without LUTS. With the recent introduction of the new species Lactobacillus mulieris, several strains of L. jensenii were reclassified as L. mulieris based upon gene marker and average nucleotide identity. We took a phylogenomic and comparative genomic approach to ascertain the genetic determinants of these two species. Looking at a larger data set, we identified additional L. mulieris strains, including one distinct from other members of the species—L. mulieris UMB7784. Furthermore, we identified unique loci in each species that may have clinical implications.
format	article
author	Catherine Putonti Jason W. Shapiro Adriana Ene Oleksandra Tsibere Alan J. Wolfe
author_facet	Catherine Putonti Jason W. Shapiro Adriana Ene Oleksandra Tsibere Alan J. Wolfe
author_sort	Catherine Putonti
title	Comparative Genomic Study of <named-content content-type="genus-species">Lactobacillus jensenii</named-content> and the Newly Defined <named-content content-type="genus-species">Lactobacillus mulieris</named-content> Species Identifies Species-Specific Functionality
title_short	Comparative Genomic Study of <named-content content-type="genus-species">Lactobacillus jensenii</named-content> and the Newly Defined <named-content content-type="genus-species">Lactobacillus mulieris</named-content> Species Identifies Species-Specific Functionality
title_full	Comparative Genomic Study of <named-content content-type="genus-species">Lactobacillus jensenii</named-content> and the Newly Defined <named-content content-type="genus-species">Lactobacillus mulieris</named-content> Species Identifies Species-Specific Functionality
title_fullStr	Comparative Genomic Study of <named-content content-type="genus-species">Lactobacillus jensenii</named-content> and the Newly Defined <named-content content-type="genus-species">Lactobacillus mulieris</named-content> Species Identifies Species-Specific Functionality
title_full_unstemmed	Comparative Genomic Study of <named-content content-type="genus-species">Lactobacillus jensenii</named-content> and the Newly Defined <named-content content-type="genus-species">Lactobacillus mulieris</named-content> Species Identifies Species-Specific Functionality
title_sort	comparative genomic study of <named-content content-type="genus-species">lactobacillus jensenii</named-content> and the newly defined <named-content content-type="genus-species">lactobacillus mulieris</named-content> species identifies species-specific functionality
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/80c3593f88e74079833541c4d403eaee
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Comparative Genomic Study of <named-content content-type="genus-species">Lactobacillus jensenii</named-content> and the Newly Defined <named-content content-type="genus-species">Lactobacillus mulieris</named-content> Species Identifies Species-Specific Functionality

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