Expression of CIB1 correlates with colorectal liver metastases but not with peritoneal carcinomatosis

Abstract Background Molecular differences in colorectal cancer (CRC) are associated with the metastatic route. Patient survival is mainly driven by metastatic spread thus it is imperative to understand its key drivers to develop biomarkers for risk stratification, follow-up protocols and personalize...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sven Jacob, Florian Bösch, Markus B. Schoenberg, Elise Pretzsch, Christopher Lampert, Ren Haoyu, Bernhard W. Renz, Marlies Michl, Jörg Kumbrink, Thomas Kirchner, Jens Werner, Martin K. Angele, Jens Neumann
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/80c7cc8532c74acdb1342db764e9e655
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:80c7cc8532c74acdb1342db764e9e655
record_format	dspace
spelling	oai:doaj.org-article:80c7cc8532c74acdb1342db764e9e6552021-11-21T12:30:08ZExpression of CIB1 correlates with colorectal liver metastases but not with peritoneal carcinomatosis10.1186/s12885-021-08927-w1471-2407https://doaj.org/article/80c7cc8532c74acdb1342db764e9e6552021-11-01T00:00:00Zhttps://doi.org/10.1186/s12885-021-08927-whttps://doaj.org/toc/1471-2407Abstract Background Molecular differences in colorectal cancer (CRC) are associated with the metastatic route. Patient survival is mainly driven by metastatic spread thus it is imperative to understand its key drivers to develop biomarkers for risk stratification, follow-up protocols and personalized therapy. Thus, this study aimed to identify genes associated with the metastatic route in CRC. Material and methods CRC patients resected at our clinic from 2005 to 2014 and with a minimum 5-year follow-up were included in this analysis and grouped into CRC with hepatic (HEP), peritoneal (PER) or without distant metastases (M0), and HEP/PER. Firstly, tumor RNA of 6 patients each was isolated by microdissection from formalin-fixed paraffin-embedded specimens and analyzed by a NanoString analysis. Subsequently, these results were validated with immunohistochemistry and correlated to clinicopathological parameters in a larger collective of CRC patients (HEP n = 51, PER n = 44, M0 n = 47, HEP/PER n = 28). Results Compared to M0, HEP tumors showed 20 differentially expressed genes associated with epithelial-mesenchymal transition (EMT) and angiogenesis. Compared to M0, PER tumors had 18 differentially expressed genes. The finding of different gene signatures was supported by the multidimensional principal component clustering analysis. Tumor perforation did not influence the metastatic route. CIB1 was homogenously and significantly overexpressed in HEP compared to M0 (p < 0.001), but not in PER. Furthermore, immunohistochemical validation demonstrated that the mean CIB1 expression in HEP was 80% higher than in M0 (p < 0.001). Conclusion Gene expression analysis revealed that CIB1 is significantly overexpressed in CRC leading to liver metastases compared to M0 and PER. Thus, the present results suggest that CIB1 may play a crucial role for hematogenous spread to the liver but not for peritoneal carcinomatosis. Consequently, CIB1 seems to be a promising prognostic marker and a potential tool for future targeted therapies as well as early diagnostics and follow-up.Sven JacobFlorian BöschMarkus B. SchoenbergElise PretzschChristopher LampertRen HaoyuBernhard W. RenzMarlies MichlJörg KumbrinkThomas KirchnerJens WernerMartin K. AngeleJens NeumannBMCarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBMC Cancer, Vol 21, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Sven Jacob Florian Bösch Markus B. Schoenberg Elise Pretzsch Christopher Lampert Ren Haoyu Bernhard W. Renz Marlies Michl Jörg Kumbrink Thomas Kirchner Jens Werner Martin K. Angele Jens Neumann Expression of CIB1 correlates with colorectal liver metastases but not with peritoneal carcinomatosis
description	Abstract Background Molecular differences in colorectal cancer (CRC) are associated with the metastatic route. Patient survival is mainly driven by metastatic spread thus it is imperative to understand its key drivers to develop biomarkers for risk stratification, follow-up protocols and personalized therapy. Thus, this study aimed to identify genes associated with the metastatic route in CRC. Material and methods CRC patients resected at our clinic from 2005 to 2014 and with a minimum 5-year follow-up were included in this analysis and grouped into CRC with hepatic (HEP), peritoneal (PER) or without distant metastases (M0), and HEP/PER. Firstly, tumor RNA of 6 patients each was isolated by microdissection from formalin-fixed paraffin-embedded specimens and analyzed by a NanoString analysis. Subsequently, these results were validated with immunohistochemistry and correlated to clinicopathological parameters in a larger collective of CRC patients (HEP n = 51, PER n = 44, M0 n = 47, HEP/PER n = 28). Results Compared to M0, HEP tumors showed 20 differentially expressed genes associated with epithelial-mesenchymal transition (EMT) and angiogenesis. Compared to M0, PER tumors had 18 differentially expressed genes. The finding of different gene signatures was supported by the multidimensional principal component clustering analysis. Tumor perforation did not influence the metastatic route. CIB1 was homogenously and significantly overexpressed in HEP compared to M0 (p < 0.001), but not in PER. Furthermore, immunohistochemical validation demonstrated that the mean CIB1 expression in HEP was 80% higher than in M0 (p < 0.001). Conclusion Gene expression analysis revealed that CIB1 is significantly overexpressed in CRC leading to liver metastases compared to M0 and PER. Thus, the present results suggest that CIB1 may play a crucial role for hematogenous spread to the liver but not for peritoneal carcinomatosis. Consequently, CIB1 seems to be a promising prognostic marker and a potential tool for future targeted therapies as well as early diagnostics and follow-up.
format	article
author	Sven Jacob Florian Bösch Markus B. Schoenberg Elise Pretzsch Christopher Lampert Ren Haoyu Bernhard W. Renz Marlies Michl Jörg Kumbrink Thomas Kirchner Jens Werner Martin K. Angele Jens Neumann
author_facet	Sven Jacob Florian Bösch Markus B. Schoenberg Elise Pretzsch Christopher Lampert Ren Haoyu Bernhard W. Renz Marlies Michl Jörg Kumbrink Thomas Kirchner Jens Werner Martin K. Angele Jens Neumann
author_sort	Sven Jacob
title	Expression of CIB1 correlates with colorectal liver metastases but not with peritoneal carcinomatosis
title_short	Expression of CIB1 correlates with colorectal liver metastases but not with peritoneal carcinomatosis
title_full	Expression of CIB1 correlates with colorectal liver metastases but not with peritoneal carcinomatosis
title_fullStr	Expression of CIB1 correlates with colorectal liver metastases but not with peritoneal carcinomatosis
title_full_unstemmed	Expression of CIB1 correlates with colorectal liver metastases but not with peritoneal carcinomatosis
title_sort	expression of cib1 correlates with colorectal liver metastases but not with peritoneal carcinomatosis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/80c7cc8532c74acdb1342db764e9e655
work_keys_str_mv	AT svenjacob expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis AT florianbosch expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis AT markusbschoenberg expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis AT elisepretzsch expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis AT christopherlampert expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis AT renhaoyu expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis AT bernhardwrenz expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis AT marliesmichl expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis AT jorgkumbrink expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis AT thomaskirchner expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis AT jenswerner expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis AT martinkangele expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis AT jensneumann expressionofcib1correlateswithcolorectallivermetastasesbutnotwithperitonealcarcinomatosis
_version_	1718418954863509504

Expression of CIB1 correlates with colorectal liver metastases but not with peritoneal carcinomatosis

Ejemplares similares