The Protective Effect of Taurine on Oxidized Fish-Oil-Induced Liver Oxidative Stress and Intestinal Barrier-Function Impairment in Juvenile <i>Ictalurus punctatus</i>
Dietary lipids provide energy for growth and development and provide fatty acids necessary for normal structure and biological function. However, oxidized lipids cause oxidative stress and intestinal damage. An 8-week feeding trial with fresh fish oil (FFO, control group), oxidized fish oil (OFO), a...
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/80d47c3dfe5f42be8e01a724863d22ca |
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| Sumario: | Dietary lipids provide energy for growth and development and provide fatty acids necessary for normal structure and biological function. However, oxidized lipids cause oxidative stress and intestinal damage. An 8-week feeding trial with fresh fish oil (FFO, control group), oxidized fish oil (OFO), and taurine-supplemented diets (OFOT, OFO + 0.2% of taurine) was conducted to evaluate the protective effect of taurine on oxidized fish-oil-induced liver oxidative stress and intestine impairment in juvenile <i>I</i><i>ctalurus</i><i>pun</i><i>cta</i><i>tus</i>. The results showed that (1) Growth performance was significantly lower in fish fed OFO than in those fed other diets, whereas the opposite occurred in the hepatosomatic index. (2) OFO-feeding significantly increased lipid deposition compared with the FFO group. The addition of taurine ameliorated the OFO-induced increase in lipid vacuolization in the liver, significantly upregulated <i>lpl</i> mRNA expression, and downregulated <i>fas</i> and <i>srebp1</i> mRNA expression. (3) OFO-feeding significantly reduced oxidative damage of liver. Compared with the OFO group, the OFOT group remarkably upregulated antioxidant enzyme mRNA expression through the Nrf2-Keap1 signaling pathway based on the transcriptional expression. (4) OFO diets induced intestinal physical and immune barrier damage. Compared with the OFO group, OFOT diets remarkably downregulated <i>il-1β</i>, <i>il-6</i>, <i>tnf-α</i>, and <i>il-8</i> mRNA expression and upregulated <i>tgf-β</i> mRNA expression through the NF-κB signaling pathway. Besides, the addition of taurine to OFO diets significantly upregulated <i>zo-2</i> and <i>zo-1</i> mRNA expression, and downregulated <i>claudin-15</i> and <i>claudin-12</i> mRNA expression. In conclusion, oxidized-fish-oil diets can cause negative physiological health effects in <i>I</i><i>ctalurus</i><i>pun</i><i>cta</i><i>tus</i>, while adding taurine can increase growth and antioxidant ability, reduce lipid deposition, and improve intestinal health. |
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