Biochemical Changes during Plantlet Regeneration in Two Accessions of Mucuna pruriens

The genus Mucuna is an important medicinal herb and is extensively used in traditional Indian systems of medicine for various ailments. In vitro culture technique provides an alternative to plant propagation and germplasm conservation. Our aim was to study the biochemical changes occurring during re...

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Autores principales:	S Raghavendra, C K Ramesh, V Kumar, M H M Khan, B S Harish
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2015
Materias:	mucuna in vitro biochemical changes regeneration enzymes Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/80df54064a854fa0851d929e802057dd
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spelling	oai:doaj.org-article:80df54064a854fa0851d929e802057dd2021-12-02T12:37:43ZBiochemical Changes during Plantlet Regeneration in Two Accessions of Mucuna pruriens0973-354X2582-4899https://doaj.org/article/80df54064a854fa0851d929e802057dd2015-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/144https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899The genus Mucuna is an important medicinal herb and is extensively used in traditional Indian systems of medicine for various ailments. In vitro culture technique provides an alternative to plant propagation and germplasm conservation. Our aim was to study the biochemical changes occurring during regeneration of shoots (plantlets) from explants of two accessions of Mucuna pruriens, by monitoring the efficiency of nitrogen utilization and changes in levels of some hydrolytic enzymes. A rapid micropropagation system was developed using Murashige and Skoog's (MS) medium supplemented with BAP and IAA combined. In both the accessions, 3.0mg l-1 6-BAP, in combination with 0.2mg l-1 IAA, induced shoot buds and shoot elongation; however for multiple-shoot induction, a slightly higher concentration of cytokinin, i.e., 3.5mg l-16-BAP, in combination with 0.2mg l-1 IAA, was required. Results of the present study confirm an active growth of explants revealed by nitrate assimilation enzymes and hydrolytic enzymes. It is concluded that medium composition, growth regulator combination and culture incubation conditions are all vital in both the accessions of Mucuna pruriens for induction of in vitro plant regeneration.S RaghavendraC K RameshV KumarM H M KhanB S HarishSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlemucunain vitrobiochemical changesregenerationenzymesPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 10, Iss 1, Pp 1-7 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	mucuna in vitro biochemical changes regeneration enzymes Plant culture SB1-1110
spellingShingle	mucuna in vitro biochemical changes regeneration enzymes Plant culture SB1-1110 S Raghavendra C K Ramesh V Kumar M H M Khan B S Harish Biochemical Changes during Plantlet Regeneration in Two Accessions of Mucuna pruriens
description	The genus Mucuna is an important medicinal herb and is extensively used in traditional Indian systems of medicine for various ailments. In vitro culture technique provides an alternative to plant propagation and germplasm conservation. Our aim was to study the biochemical changes occurring during regeneration of shoots (plantlets) from explants of two accessions of Mucuna pruriens, by monitoring the efficiency of nitrogen utilization and changes in levels of some hydrolytic enzymes. A rapid micropropagation system was developed using Murashige and Skoog's (MS) medium supplemented with BAP and IAA combined. In both the accessions, 3.0mg l-1 6-BAP, in combination with 0.2mg l-1 IAA, induced shoot buds and shoot elongation; however for multiple-shoot induction, a slightly higher concentration of cytokinin, i.e., 3.5mg l-16-BAP, in combination with 0.2mg l-1 IAA, was required. Results of the present study confirm an active growth of explants revealed by nitrate assimilation enzymes and hydrolytic enzymes. It is concluded that medium composition, growth regulator combination and culture incubation conditions are all vital in both the accessions of Mucuna pruriens for induction of in vitro plant regeneration.
format	article
author	S Raghavendra C K Ramesh V Kumar M H M Khan B S Harish
author_facet	S Raghavendra C K Ramesh V Kumar M H M Khan B S Harish
author_sort	S Raghavendra
title	Biochemical Changes during Plantlet Regeneration in Two Accessions of Mucuna pruriens
title_short	Biochemical Changes during Plantlet Regeneration in Two Accessions of Mucuna pruriens
title_full	Biochemical Changes during Plantlet Regeneration in Two Accessions of Mucuna pruriens
title_fullStr	Biochemical Changes during Plantlet Regeneration in Two Accessions of Mucuna pruriens
title_full_unstemmed	Biochemical Changes during Plantlet Regeneration in Two Accessions of Mucuna pruriens
title_sort	biochemical changes during plantlet regeneration in two accessions of mucuna pruriens
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2015
url	https://doaj.org/article/80df54064a854fa0851d929e802057dd
work_keys_str_mv	AT sraghavendra biochemicalchangesduringplantletregenerationintwoaccessionsofmucunapruriens AT ckramesh biochemicalchangesduringplantletregenerationintwoaccessionsofmucunapruriens AT vkumar biochemicalchangesduringplantletregenerationintwoaccessionsofmucunapruriens AT mhmkhan biochemicalchangesduringplantletregenerationintwoaccessionsofmucunapruriens AT bsharish biochemicalchangesduringplantletregenerationintwoaccessionsofmucunapruriens
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Biochemical Changes during Plantlet Regeneration in Two Accessions of Mucuna pruriens

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