Inhibition of miR-155 Promotes TGF-β Mediated Suppression of HIV Release in the Cervical Epithelial Cells

TGF-β has been shown to play a differential role in either restricting or aiding HIV infection in different cell types, however its role in the cervical cells is hitherto undefined. Among females, more than 80% of infections occur through heterosexual contact where cervicovaginal mucosa plays a crit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jyotsna Gokavi, Sharwari Sadawarte, Anant Shelke, Urmila Kulkarni-Kale, Madhuri Thakar, Vandana Saxena
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	HIV cervical TGF-β microRNA host restriction factors miR-155 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/80df93be959f469babbdb58708ec9406
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:80df93be959f469babbdb58708ec9406
record_format	dspace
spelling	oai:doaj.org-article:80df93be959f469babbdb58708ec94062021-11-25T19:13:55ZInhibition of miR-155 Promotes TGF-β Mediated Suppression of HIV Release in the Cervical Epithelial Cells10.3390/v131122661999-4915https://doaj.org/article/80df93be959f469babbdb58708ec94062021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2266https://doaj.org/toc/1999-4915TGF-β has been shown to play a differential role in either restricting or aiding HIV infection in different cell types, however its role in the cervical cells is hitherto undefined. Among females, more than 80% of infections occur through heterosexual contact where cervicovaginal mucosa plays a critical role, however the early events during the establishment of infection at female genital mucosa are poorly understood. We earlier showed that increased TGF-β level has been associated with cervical viral shedding in the HIV infected women, however a causal relationship could not be examined. Therefore, here we first established an in vitro cell-associated model of HIV infection in the cervical epithelial cells (ME-180) and demonstrated that TGF-β plays an important role as a negative regulator of HIV release in the infected cervical epithelial cells. Inhibition of miR-155 upregulated TGF-β signaling and mRNA expression of host restriction factors such as <i>APOBEC-3G</i>, <i>IFI-16</i> and <i>IFITM-3</i>, while decreased the HIV release in ME-180 cells. To conclude, this is the first study to decipher the complex interplay between TGF-β, miR-155 and HIV release in the cervical epithelial cells. Collectively, our data suggest the plausible role of TGF-β in promoting HIV latency in cervical epithelial cells which needs further investigations.Jyotsna GokaviSharwari SadawarteAnant ShelkeUrmila Kulkarni-KaleMadhuri ThakarVandana SaxenaMDPI AGarticleHIVcervicalTGF-βmicroRNAhost restriction factorsmiR-155MicrobiologyQR1-502ENViruses, Vol 13, Iss 2266, p 2266 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HIV cervical TGF-β microRNA host restriction factors miR-155 Microbiology QR1-502
spellingShingle	HIV cervical TGF-β microRNA host restriction factors miR-155 Microbiology QR1-502 Jyotsna Gokavi Sharwari Sadawarte Anant Shelke Urmila Kulkarni-Kale Madhuri Thakar Vandana Saxena Inhibition of miR-155 Promotes TGF-β Mediated Suppression of HIV Release in the Cervical Epithelial Cells
description	TGF-β has been shown to play a differential role in either restricting or aiding HIV infection in different cell types, however its role in the cervical cells is hitherto undefined. Among females, more than 80% of infections occur through heterosexual contact where cervicovaginal mucosa plays a critical role, however the early events during the establishment of infection at female genital mucosa are poorly understood. We earlier showed that increased TGF-β level has been associated with cervical viral shedding in the HIV infected women, however a causal relationship could not be examined. Therefore, here we first established an in vitro cell-associated model of HIV infection in the cervical epithelial cells (ME-180) and demonstrated that TGF-β plays an important role as a negative regulator of HIV release in the infected cervical epithelial cells. Inhibition of miR-155 upregulated TGF-β signaling and mRNA expression of host restriction factors such as <i>APOBEC-3G</i>, <i>IFI-16</i> and <i>IFITM-3</i>, while decreased the HIV release in ME-180 cells. To conclude, this is the first study to decipher the complex interplay between TGF-β, miR-155 and HIV release in the cervical epithelial cells. Collectively, our data suggest the plausible role of TGF-β in promoting HIV latency in cervical epithelial cells which needs further investigations.
format	article
author	Jyotsna Gokavi Sharwari Sadawarte Anant Shelke Urmila Kulkarni-Kale Madhuri Thakar Vandana Saxena
author_facet	Jyotsna Gokavi Sharwari Sadawarte Anant Shelke Urmila Kulkarni-Kale Madhuri Thakar Vandana Saxena
author_sort	Jyotsna Gokavi
title	Inhibition of miR-155 Promotes TGF-β Mediated Suppression of HIV Release in the Cervical Epithelial Cells
title_short	Inhibition of miR-155 Promotes TGF-β Mediated Suppression of HIV Release in the Cervical Epithelial Cells
title_full	Inhibition of miR-155 Promotes TGF-β Mediated Suppression of HIV Release in the Cervical Epithelial Cells
title_fullStr	Inhibition of miR-155 Promotes TGF-β Mediated Suppression of HIV Release in the Cervical Epithelial Cells
title_full_unstemmed	Inhibition of miR-155 Promotes TGF-β Mediated Suppression of HIV Release in the Cervical Epithelial Cells
title_sort	inhibition of mir-155 promotes tgf-β mediated suppression of hiv release in the cervical epithelial cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/80df93be959f469babbdb58708ec9406
work_keys_str_mv	AT jyotsnagokavi inhibitionofmir155promotestgfbmediatedsuppressionofhivreleaseinthecervicalepithelialcells AT sharwarisadawarte inhibitionofmir155promotestgfbmediatedsuppressionofhivreleaseinthecervicalepithelialcells AT anantshelke inhibitionofmir155promotestgfbmediatedsuppressionofhivreleaseinthecervicalepithelialcells AT urmilakulkarnikale inhibitionofmir155promotestgfbmediatedsuppressionofhivreleaseinthecervicalepithelialcells AT madhurithakar inhibitionofmir155promotestgfbmediatedsuppressionofhivreleaseinthecervicalepithelialcells AT vandanasaxena inhibitionofmir155promotestgfbmediatedsuppressionofhivreleaseinthecervicalepithelialcells
_version_	1718410166688284672

Inhibition of miR-155 Promotes TGF-β Mediated Suppression of HIV Release in the Cervical Epithelial Cells

Ejemplares similares