Differential compartmentalization of HIV-targeting immune cells in inner and outer foreskin tissue.

Ex vivo foreskin models have demonstrated that inner foreskin is more susceptible to HIV-1 infection than outer foreskin. In the present study we characterized the compartition of HIV-1 target cells and quantified these cells in the epidermis and dermis of inner and outer foreskins using immunohisto...

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Autores principales:	Aiping Liu, Yu Yang, Lu Liu, Zhefeng Meng, Liangzhu Li, Chao Qiu, Jianqing Xu, Xiaoyan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/80e477dac96743a3ab47798b20ec53ec
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spelling	oai:doaj.org-article:80e477dac96743a3ab47798b20ec53ec2021-11-18T08:37:43ZDifferential compartmentalization of HIV-targeting immune cells in inner and outer foreskin tissue.1932-620310.1371/journal.pone.0085176https://doaj.org/article/80e477dac96743a3ab47798b20ec53ec2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24454812/?tool=EBIhttps://doaj.org/toc/1932-6203Ex vivo foreskin models have demonstrated that inner foreskin is more susceptible to HIV-1 infection than outer foreskin. In the present study we characterized the compartition of HIV-1 target cells and quantified these cells in the epidermis and dermis of inner and outer foreskins using immunohistochemistry and flow cytometry. Our data showed that the epidermis of the inner foreskin was more enriched with CD4(+) T cells and Langerhans cells (LCs), with the co-expression of CCR5 and α4β7 receptors, than the outer foreskin. Interestingly, the vast majority of CD4(+) T cells and LCs expressed CCR5, but not CXCR4, indicating that the inner foreskin might capture and transmit R5-tropic HIV strains more efficiently. In addition, lymphoid aggregates, composed of T cells, macrophages and dendritic cells (DCs) in the dermis, were closer to the epithelial surface in the inner foreskin than in the outer foreskin. As dendritic cells are able to capture and pass HIV particles to susceptible target cells, HIV may be able to more efficiently infect the inner foreskin by hijacking the augmented immune communication pathways in this tissue. After the inoculation of HIV-1 particles in a foreskin explant culture model, the level of p24 antigen in the supernatant from the inner foreskin was slightly higher than that from the outer foreskin, although this difference was not significant. The present study is the first to employ both CCR5 and α4β7 to identify HIV target cells in the foreskin. Our data demonstrated that the inner foreskin was more enriched with HIV target immune cells than the outer foreskin, and this tissue was structured for efficient communication among immune cells that may promote HIV transmission and replication. In addition, our data suggests the R5-tropism of HIV sexual transmission is likely shaped through the inherent receptor composition on HIV target cells in the mucosa.Aiping LiuYu YangLu LiuZhefeng MengLiangzhu LiChao QiuJianqing XuXiaoyan ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e85176 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aiping Liu Yu Yang Lu Liu Zhefeng Meng Liangzhu Li Chao Qiu Jianqing Xu Xiaoyan Zhang Differential compartmentalization of HIV-targeting immune cells in inner and outer foreskin tissue.
description	Ex vivo foreskin models have demonstrated that inner foreskin is more susceptible to HIV-1 infection than outer foreskin. In the present study we characterized the compartition of HIV-1 target cells and quantified these cells in the epidermis and dermis of inner and outer foreskins using immunohistochemistry and flow cytometry. Our data showed that the epidermis of the inner foreskin was more enriched with CD4(+) T cells and Langerhans cells (LCs), with the co-expression of CCR5 and α4β7 receptors, than the outer foreskin. Interestingly, the vast majority of CD4(+) T cells and LCs expressed CCR5, but not CXCR4, indicating that the inner foreskin might capture and transmit R5-tropic HIV strains more efficiently. In addition, lymphoid aggregates, composed of T cells, macrophages and dendritic cells (DCs) in the dermis, were closer to the epithelial surface in the inner foreskin than in the outer foreskin. As dendritic cells are able to capture and pass HIV particles to susceptible target cells, HIV may be able to more efficiently infect the inner foreskin by hijacking the augmented immune communication pathways in this tissue. After the inoculation of HIV-1 particles in a foreskin explant culture model, the level of p24 antigen in the supernatant from the inner foreskin was slightly higher than that from the outer foreskin, although this difference was not significant. The present study is the first to employ both CCR5 and α4β7 to identify HIV target cells in the foreskin. Our data demonstrated that the inner foreskin was more enriched with HIV target immune cells than the outer foreskin, and this tissue was structured for efficient communication among immune cells that may promote HIV transmission and replication. In addition, our data suggests the R5-tropism of HIV sexual transmission is likely shaped through the inherent receptor composition on HIV target cells in the mucosa.
format	article
author	Aiping Liu Yu Yang Lu Liu Zhefeng Meng Liangzhu Li Chao Qiu Jianqing Xu Xiaoyan Zhang
author_facet	Aiping Liu Yu Yang Lu Liu Zhefeng Meng Liangzhu Li Chao Qiu Jianqing Xu Xiaoyan Zhang
author_sort	Aiping Liu
title	Differential compartmentalization of HIV-targeting immune cells in inner and outer foreskin tissue.
title_short	Differential compartmentalization of HIV-targeting immune cells in inner and outer foreskin tissue.
title_full	Differential compartmentalization of HIV-targeting immune cells in inner and outer foreskin tissue.
title_fullStr	Differential compartmentalization of HIV-targeting immune cells in inner and outer foreskin tissue.
title_full_unstemmed	Differential compartmentalization of HIV-targeting immune cells in inner and outer foreskin tissue.
title_sort	differential compartmentalization of hiv-targeting immune cells in inner and outer foreskin tissue.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/80e477dac96743a3ab47798b20ec53ec
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Differential compartmentalization of HIV-targeting immune cells in inner and outer foreskin tissue.

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