Optical tissue clearing enables rapid, precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research

Morphological analyses are key outcome assessments for nerve regeneration studies but are historically limited to tissue sections. Novel optical tissue clearing techniques enabling three-dimensional imaging of entire organs at a subcellular resolution have revolutionized morphological studies of the...

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Autores principales:	Simeon C Daeschler, Jennifer Zhang, Tessa Gordon, Gregory H Borschel
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2022
Materias:	histology; image segmentation; morphometry; nerve regeneration; outcome assessment; peripheral nerve; three-dimensional imaging; tissue clearing Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/80eb15f4486846fb9f02682236376ab8
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spelling	oai:doaj.org-article:80eb15f4486846fb9f02682236376ab82021-11-19T12:16:44ZOptical tissue clearing enables rapid, precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research1673-537410.4103/1673-5374.329473https://doaj.org/article/80eb15f4486846fb9f02682236376ab82022-01-01T00:00:00Zhttp://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=6;spage=1348;epage=1356;aulast=Daeschlerhttps://doaj.org/toc/1673-5374Morphological analyses are key outcome assessments for nerve regeneration studies but are historically limited to tissue sections. Novel optical tissue clearing techniques enabling three-dimensional imaging of entire organs at a subcellular resolution have revolutionized morphological studies of the brain. To extend their applicability to experimental nerve repair studies we adapted these techniques to nerves and their motor and sensory targets in rats. The solvent-based protocols rendered harvested peripheral nerves and their target organs transparent within 24 hours while preserving tissue architecture and fluorescence. The optical clearing was compatible with conventional laboratory techniques, including retrograde labeling studies, and computational image segmentation, providing fast and precise cell quantitation. Further, optically cleared organs enabled three-dimensional morphometry at an unprecedented scale including dermatome-wide innervation studies, tracing of intramuscular nerve branches or mapping of neurovascular networks. Given their wide-ranging applicability, rapid processing times, and low costs, tissue clearing techniques are likely to be a key technology for next-generation nerve repair studies. All procedures were approved by the Hospital for Sick Children’s Laboratory Animal Services Committee (49871/9) on November 9, 2019.Simeon C DaeschlerJennifer ZhangTessa GordonGregory H BorschelWolters Kluwer Medknow Publicationsarticlehistology; image segmentation; morphometry; nerve regeneration; outcome assessment; peripheral nerve; three-dimensional imaging; tissue clearingNeurology. Diseases of the nervous systemRC346-429ENNeural Regeneration Research, Vol 17, Iss 6, Pp 1348-1356 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	histology; image segmentation; morphometry; nerve regeneration; outcome assessment; peripheral nerve; three-dimensional imaging; tissue clearing Neurology. Diseases of the nervous system RC346-429
spellingShingle	histology; image segmentation; morphometry; nerve regeneration; outcome assessment; peripheral nerve; three-dimensional imaging; tissue clearing Neurology. Diseases of the nervous system RC346-429 Simeon C Daeschler Jennifer Zhang Tessa Gordon Gregory H Borschel Optical tissue clearing enables rapid, precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research
description	Morphological analyses are key outcome assessments for nerve regeneration studies but are historically limited to tissue sections. Novel optical tissue clearing techniques enabling three-dimensional imaging of entire organs at a subcellular resolution have revolutionized morphological studies of the brain. To extend their applicability to experimental nerve repair studies we adapted these techniques to nerves and their motor and sensory targets in rats. The solvent-based protocols rendered harvested peripheral nerves and their target organs transparent within 24 hours while preserving tissue architecture and fluorescence. The optical clearing was compatible with conventional laboratory techniques, including retrograde labeling studies, and computational image segmentation, providing fast and precise cell quantitation. Further, optically cleared organs enabled three-dimensional morphometry at an unprecedented scale including dermatome-wide innervation studies, tracing of intramuscular nerve branches or mapping of neurovascular networks. Given their wide-ranging applicability, rapid processing times, and low costs, tissue clearing techniques are likely to be a key technology for next-generation nerve repair studies. All procedures were approved by the Hospital for Sick Children’s Laboratory Animal Services Committee (49871/9) on November 9, 2019.
format	article
author	Simeon C Daeschler Jennifer Zhang Tessa Gordon Gregory H Borschel
author_facet	Simeon C Daeschler Jennifer Zhang Tessa Gordon Gregory H Borschel
author_sort	Simeon C Daeschler
title	Optical tissue clearing enables rapid, precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research
title_short	Optical tissue clearing enables rapid, precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research
title_full	Optical tissue clearing enables rapid, precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research
title_fullStr	Optical tissue clearing enables rapid, precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research
title_full_unstemmed	Optical tissue clearing enables rapid, precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research
title_sort	optical tissue clearing enables rapid, precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research
publisher	Wolters Kluwer Medknow Publications
publishDate	2022
url	https://doaj.org/article/80eb15f4486846fb9f02682236376ab8
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Optical tissue clearing enables rapid, precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research

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