Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA

Abstract Feline leukemia virus (FeLV) is a major viral disease in cats, causing leukemia and lymphoma. The molecular detection of FeLV RNA and the DNA provirus are important for staging of the disease. However, the rapid immunochromatographic assay commonly used for antigen detection can only detect...

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Autores principales:	Sitthichok Lacharoje, Somporn Techangamsuwan, Nuntaree Chaichanawongsaroj
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/80f35f70e88440a6b09b8d04ffae1c60
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spelling	oai:doaj.org-article:80f35f70e88440a6b09b8d04ffae1c602021-11-14T12:19:50ZRapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA10.1038/s41598-021-01585-92045-2322https://doaj.org/article/80f35f70e88440a6b09b8d04ffae1c602021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01585-9https://doaj.org/toc/2045-2322Abstract Feline leukemia virus (FeLV) is a major viral disease in cats, causing leukemia and lymphoma. The molecular detection of FeLV RNA and the DNA provirus are important for staging of the disease. However, the rapid immunochromatographic assay commonly used for antigen detection can only detect viremia at the progressive stage. In this study, nested recombinase polymerase amplification (nRPA) was developed for exogenous FeLV DNA provirus detection, and reverse transcriptase polymerase amplification (RT-RPA) was developed for the detection of FeLV RNA. The approaches were validated using 108 cats with clinicopathologic abnormalities due to FeLV infection, and from 14 healthy cats in a vaccination plan. The nRPA and RT-RPA assays could rapidly amplify the FeLV template, and produced high sensitivity and specificity. The FeLV detection rate in regression cats by nRPA was increased up to 45.8% compared to the rapid immunochromatographic assay. Hence, FeLV diagnosis using nRPA and RT-RPA are rapid and easily established in low resource settings, benefiting FeLV prognosis, prevention, and control of both horizontal and vertical transmission.Sitthichok LacharojeSomporn TechangamsuwanNuntaree ChaichanawongsarojNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sitthichok Lacharoje Somporn Techangamsuwan Nuntaree Chaichanawongsaroj Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA
description	Abstract Feline leukemia virus (FeLV) is a major viral disease in cats, causing leukemia and lymphoma. The molecular detection of FeLV RNA and the DNA provirus are important for staging of the disease. However, the rapid immunochromatographic assay commonly used for antigen detection can only detect viremia at the progressive stage. In this study, nested recombinase polymerase amplification (nRPA) was developed for exogenous FeLV DNA provirus detection, and reverse transcriptase polymerase amplification (RT-RPA) was developed for the detection of FeLV RNA. The approaches were validated using 108 cats with clinicopathologic abnormalities due to FeLV infection, and from 14 healthy cats in a vaccination plan. The nRPA and RT-RPA assays could rapidly amplify the FeLV template, and produced high sensitivity and specificity. The FeLV detection rate in regression cats by nRPA was increased up to 45.8% compared to the rapid immunochromatographic assay. Hence, FeLV diagnosis using nRPA and RT-RPA are rapid and easily established in low resource settings, benefiting FeLV prognosis, prevention, and control of both horizontal and vertical transmission.
format	article
author	Sitthichok Lacharoje Somporn Techangamsuwan Nuntaree Chaichanawongsaroj
author_facet	Sitthichok Lacharoje Somporn Techangamsuwan Nuntaree Chaichanawongsaroj
author_sort	Sitthichok Lacharoje
title	Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA
title_short	Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA
title_full	Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA
title_fullStr	Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA
title_full_unstemmed	Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA
title_sort	rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (rpa) and reverse transcriptase-rpa
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/80f35f70e88440a6b09b8d04ffae1c60
work_keys_str_mv	AT sitthichoklacharoje rapidcharacterizationoffelineleukemiavirusinfectivestagesbyanovelnestedrecombinasepolymeraseamplificationrpaandreversetranscriptaserpa AT somporntechangamsuwan rapidcharacterizationoffelineleukemiavirusinfectivestagesbyanovelnestedrecombinasepolymeraseamplificationrpaandreversetranscriptaserpa AT nuntareechaichanawongsaroj rapidcharacterizationoffelineleukemiavirusinfectivestagesbyanovelnestedrecombinasepolymeraseamplificationrpaandreversetranscriptaserpa
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Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA

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