Fine-tune regulation of carboxypeptidase N1 controls vascular patterning during zebrafish development

Abstract Vascular development is regulated by complicated signals and molecules in vertebrates. In this study, we characterized a novel function of carboxypeptidase N1 (Cpn1) in the vasculature. We show that cpn1 mRNA is expressed in developing vessels. The knockdown of cpn1 by morpholino injection...

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Autores principales:	Ting-Yun Wu, Yi-Shan Wang, Yi-Chun Song, Zih-Ying Chen, Yi-Ting Chen, Chien-Chih Chiu, Chang-Yi Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/80f5e5aefbc64df8951501f6d199ed4c
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spelling	oai:doaj.org-article:80f5e5aefbc64df8951501f6d199ed4c2021-12-02T16:07:47ZFine-tune regulation of carboxypeptidase N1 controls vascular patterning during zebrafish development10.1038/s41598-017-01976-x2045-2322https://doaj.org/article/80f5e5aefbc64df8951501f6d199ed4c2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01976-xhttps://doaj.org/toc/2045-2322Abstract Vascular development is regulated by complicated signals and molecules in vertebrates. In this study, we characterized a novel function of carboxypeptidase N1 (Cpn1) in the vasculature. We show that cpn1 mRNA is expressed in developing vessels. The knockdown of cpn1 by morpholino injection impairs the growth of intersegmental vessels (ISV) and caudal vein plexus (CVP), suggesting the role of cpn1 in vascular development. We showed that vascular defects are not caused by cell death but are due to the impairment of migration and proliferation. Consistent with vascular growth defects, loss of cpn1 affects the expression of the vascular markers flt4, mrc1, flk, stabilin, and ephrinb2. Furthermore, the overexpression of cpn1 impaired the growth of ISV and CVP, but the remodeling expression of vascular markers was different from the knockdown of cpn1, indicating the differential regulation mechanisms in cpn1-overexpressing embryos. We examine the interaction between cpn1 and multiple signals and observed that cpn1 is regulated by Notch/VEGF signals for ISV growth and likely regulates BMP signals for CVP patterning. In conclusion, we demonstrate that cpn1 has a critical role in the vascular development of zebrafish. We also reveal a fine-tune regulation of cpn1 that controls vascular patterning mediated by multiple signals.Ting-Yun WuYi-Shan WangYi-Chun SongZih-Ying ChenYi-Ting ChenChien-Chih ChiuChang-Yi WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ting-Yun Wu Yi-Shan Wang Yi-Chun Song Zih-Ying Chen Yi-Ting Chen Chien-Chih Chiu Chang-Yi Wu Fine-tune regulation of carboxypeptidase N1 controls vascular patterning during zebrafish development
description	Abstract Vascular development is regulated by complicated signals and molecules in vertebrates. In this study, we characterized a novel function of carboxypeptidase N1 (Cpn1) in the vasculature. We show that cpn1 mRNA is expressed in developing vessels. The knockdown of cpn1 by morpholino injection impairs the growth of intersegmental vessels (ISV) and caudal vein plexus (CVP), suggesting the role of cpn1 in vascular development. We showed that vascular defects are not caused by cell death but are due to the impairment of migration and proliferation. Consistent with vascular growth defects, loss of cpn1 affects the expression of the vascular markers flt4, mrc1, flk, stabilin, and ephrinb2. Furthermore, the overexpression of cpn1 impaired the growth of ISV and CVP, but the remodeling expression of vascular markers was different from the knockdown of cpn1, indicating the differential regulation mechanisms in cpn1-overexpressing embryos. We examine the interaction between cpn1 and multiple signals and observed that cpn1 is regulated by Notch/VEGF signals for ISV growth and likely regulates BMP signals for CVP patterning. In conclusion, we demonstrate that cpn1 has a critical role in the vascular development of zebrafish. We also reveal a fine-tune regulation of cpn1 that controls vascular patterning mediated by multiple signals.
format	article
author	Ting-Yun Wu Yi-Shan Wang Yi-Chun Song Zih-Ying Chen Yi-Ting Chen Chien-Chih Chiu Chang-Yi Wu
author_facet	Ting-Yun Wu Yi-Shan Wang Yi-Chun Song Zih-Ying Chen Yi-Ting Chen Chien-Chih Chiu Chang-Yi Wu
author_sort	Ting-Yun Wu
title	Fine-tune regulation of carboxypeptidase N1 controls vascular patterning during zebrafish development
title_short	Fine-tune regulation of carboxypeptidase N1 controls vascular patterning during zebrafish development
title_full	Fine-tune regulation of carboxypeptidase N1 controls vascular patterning during zebrafish development
title_fullStr	Fine-tune regulation of carboxypeptidase N1 controls vascular patterning during zebrafish development
title_full_unstemmed	Fine-tune regulation of carboxypeptidase N1 controls vascular patterning during zebrafish development
title_sort	fine-tune regulation of carboxypeptidase n1 controls vascular patterning during zebrafish development
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/80f5e5aefbc64df8951501f6d199ed4c
work_keys_str_mv	AT tingyunwu finetuneregulationofcarboxypeptidasen1controlsvascularpatterningduringzebrafishdevelopment AT yishanwang finetuneregulationofcarboxypeptidasen1controlsvascularpatterningduringzebrafishdevelopment AT yichunsong finetuneregulationofcarboxypeptidasen1controlsvascularpatterningduringzebrafishdevelopment AT zihyingchen finetuneregulationofcarboxypeptidasen1controlsvascularpatterningduringzebrafishdevelopment AT yitingchen finetuneregulationofcarboxypeptidasen1controlsvascularpatterningduringzebrafishdevelopment AT chienchihchiu finetuneregulationofcarboxypeptidasen1controlsvascularpatterningduringzebrafishdevelopment AT changyiwu finetuneregulationofcarboxypeptidasen1controlsvascularpatterningduringzebrafishdevelopment
_version_	1718384726767566848

Fine-tune regulation of carboxypeptidase N1 controls vascular patterning during zebrafish development

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