Population imaging discrepancies between a genetically-encoded calcium indicator (GECI) versus a genetically-encoded voltage indicator (GEVI)

Abstract Genetically-encoded calcium indicators (GECIs) are essential for studying brain function, while voltage indicators (GEVIs) are slowly permeating neuroscience. Fundamentally, GECI and GEVI measure different things, but both are advertised as reporters of “neuronal activity”. We quantified th...

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Autores principales:	Mei Hong Zhu, Jinyoung Jang, Milena M. Milosevic, Srdjan D. Antic
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/80fd080208044f89b2e5209394d636ca
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spelling	oai:doaj.org-article:80fd080208044f89b2e5209394d636ca2021-12-02T15:53:42ZPopulation imaging discrepancies between a genetically-encoded calcium indicator (GECI) versus a genetically-encoded voltage indicator (GEVI)10.1038/s41598-021-84651-62045-2322https://doaj.org/article/80fd080208044f89b2e5209394d636ca2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84651-6https://doaj.org/toc/2045-2322Abstract Genetically-encoded calcium indicators (GECIs) are essential for studying brain function, while voltage indicators (GEVIs) are slowly permeating neuroscience. Fundamentally, GECI and GEVI measure different things, but both are advertised as reporters of “neuronal activity”. We quantified the similarities and differences between calcium and voltage imaging modalities, in the context of population activity (without single-cell resolution) in brain slices. GECI optical signals showed 8–20 times better SNR than GEVI signals, but GECI signals attenuated more with distance from the stimulation site. We show the exact temporal discrepancy between calcium and voltage imaging modalities, and discuss the misleading aspects of GECI imaging. For example, population voltage signals already repolarized to the baseline (~ disappeared), while the GECI signals were still near maximum. The region-to-region propagation latencies, easily captured by GEVI imaging, are blurred in GECI imaging. Temporal summation of GECI signals is highly exaggerated, causing uniform voltage events produced by neuronal populations to appear with highly variable amplitudes in GECI population traces. Relative signal amplitudes in GECI recordings are thus misleading. In simultaneous recordings from multiple sites, the compound EPSP signals in cortical neuropil (population signals) are less distorted by GEVIs than by GECIs.Mei Hong ZhuJinyoung JangMilena M. MilosevicSrdjan D. AnticNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mei Hong Zhu Jinyoung Jang Milena M. Milosevic Srdjan D. Antic Population imaging discrepancies between a genetically-encoded calcium indicator (GECI) versus a genetically-encoded voltage indicator (GEVI)
description	Abstract Genetically-encoded calcium indicators (GECIs) are essential for studying brain function, while voltage indicators (GEVIs) are slowly permeating neuroscience. Fundamentally, GECI and GEVI measure different things, but both are advertised as reporters of “neuronal activity”. We quantified the similarities and differences between calcium and voltage imaging modalities, in the context of population activity (without single-cell resolution) in brain slices. GECI optical signals showed 8–20 times better SNR than GEVI signals, but GECI signals attenuated more with distance from the stimulation site. We show the exact temporal discrepancy between calcium and voltage imaging modalities, and discuss the misleading aspects of GECI imaging. For example, population voltage signals already repolarized to the baseline (~ disappeared), while the GECI signals were still near maximum. The region-to-region propagation latencies, easily captured by GEVI imaging, are blurred in GECI imaging. Temporal summation of GECI signals is highly exaggerated, causing uniform voltage events produced by neuronal populations to appear with highly variable amplitudes in GECI population traces. Relative signal amplitudes in GECI recordings are thus misleading. In simultaneous recordings from multiple sites, the compound EPSP signals in cortical neuropil (population signals) are less distorted by GEVIs than by GECIs.
format	article
author	Mei Hong Zhu Jinyoung Jang Milena M. Milosevic Srdjan D. Antic
author_facet	Mei Hong Zhu Jinyoung Jang Milena M. Milosevic Srdjan D. Antic
author_sort	Mei Hong Zhu
title	Population imaging discrepancies between a genetically-encoded calcium indicator (GECI) versus a genetically-encoded voltage indicator (GEVI)
title_short	Population imaging discrepancies between a genetically-encoded calcium indicator (GECI) versus a genetically-encoded voltage indicator (GEVI)
title_full	Population imaging discrepancies between a genetically-encoded calcium indicator (GECI) versus a genetically-encoded voltage indicator (GEVI)
title_fullStr	Population imaging discrepancies between a genetically-encoded calcium indicator (GECI) versus a genetically-encoded voltage indicator (GEVI)
title_full_unstemmed	Population imaging discrepancies between a genetically-encoded calcium indicator (GECI) versus a genetically-encoded voltage indicator (GEVI)
title_sort	population imaging discrepancies between a genetically-encoded calcium indicator (geci) versus a genetically-encoded voltage indicator (gevi)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/80fd080208044f89b2e5209394d636ca
work_keys_str_mv	AT meihongzhu populationimagingdiscrepanciesbetweenageneticallyencodedcalciumindicatorgeciversusageneticallyencodedvoltageindicatorgevi AT jinyoungjang populationimagingdiscrepanciesbetweenageneticallyencodedcalciumindicatorgeciversusageneticallyencodedvoltageindicatorgevi AT milenammilosevic populationimagingdiscrepanciesbetweenageneticallyencodedcalciumindicatorgeciversusageneticallyencodedvoltageindicatorgevi AT srdjandantic populationimagingdiscrepanciesbetweenageneticallyencodedcalciumindicatorgeciversusageneticallyencodedvoltageindicatorgevi
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Population imaging discrepancies between a genetically-encoded calcium indicator (GECI) versus a genetically-encoded voltage indicator (GEVI)

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