The Effects of Soyferment-Tempeh on Lipid Profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) Gene Expression in Type 2 Diabetic Mice

Diabetes Type 2 can cause oxidative stress leading to the accumulation of reactive oxygen species. Soyferment-Tempeh, a fermented soybean product with aerobic and anaerobic R. oligosporus incubation has a high antioxidant content of isoflavones that can regulate oxidative stress in diabetes. In this...

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Autores principales:	Prasetyastuti Prasetyastuti, Dian Setiawan Ghozali
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	soyferment-tempeh, lipid profile, atherogenic index, retinol-binding protein 4, phosphoenol pyruvate carboxykinase Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/81037e9b11ba476fa7b687a15cf006c2
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spelling	oai:doaj.org-article:81037e9b11ba476fa7b687a15cf006c22021-11-15T06:08:46ZThe Effects of Soyferment-Tempeh on Lipid Profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) Gene Expression in Type 2 Diabetic Mice2338-94272338-948610.22146/ijp.1354https://doaj.org/article/81037e9b11ba476fa7b687a15cf006c22021-05-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/1354https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Diabetes Type 2 can cause oxidative stress leading to the accumulation of reactive oxygen species. Soyferment-Tempeh, a fermented soybean product with aerobic and anaerobic R. oligosporus incubation has a high antioxidant content of isoflavones that can regulate oxidative stress in diabetes. In this study, we evaluate the effects of Soyferment-Tempeh on lipid profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) gene expression in type 2 diabetic mice. A total of 30 mice with the age of eight weeks were divided into six groups as follows: A) nondiabetic, B) diabetic mice, C) diabetic mice with metformin, D), E), and F) diabetic mice with Soyferment-Tempeh doses of 10, 20, or 40mg/100g body weight (BW), respectively, were administered treatments orally by gavages. Blood was collected for assessment of blood glucose level, and lipid profile before and after 3 weeks of the administration. After sacrificing the mice, livers were used for RBP4 and PEPCK gene expression assessment. Supplementation with three different doses of Soyferment-Tempeh in streptozotocin-induced diabetic mice for 21 days significantly (p<0.001) reduced blood glucose level, total cholesterol, triglycerides, low-density lipoprotein level, atherogenic index, and increased high-density lipoprotein level. There was a significant decrease in RBP4 gene expression in the Soyferment-Tempeh of dose 10mg/100g BW treatment groups (p<0.05), while the PEPCK gene expression did not significantly differ (p>0.05). These results demonstrate that supplementation with Soyferment-Tempeh decreases blood glucose level, atherogenic index, improves lipid profile, and decreases RBP4.Prasetyastuti PrasetyastutiDian Setiawan GhozaliUniversitas Gadjah Madaarticlesoyferment-tempeh, lipid profile, atherogenic index, retinol-binding protein 4, phosphoenol pyruvate carboxykinasePharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 193-200 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	soyferment-tempeh, lipid profile, atherogenic index, retinol-binding protein 4, phosphoenol pyruvate carboxykinase Pharmacy and materia medica RS1-441
spellingShingle	soyferment-tempeh, lipid profile, atherogenic index, retinol-binding protein 4, phosphoenol pyruvate carboxykinase Pharmacy and materia medica RS1-441 Prasetyastuti Prasetyastuti Dian Setiawan Ghozali The Effects of Soyferment-Tempeh on Lipid Profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) Gene Expression in Type 2 Diabetic Mice
description	Diabetes Type 2 can cause oxidative stress leading to the accumulation of reactive oxygen species. Soyferment-Tempeh, a fermented soybean product with aerobic and anaerobic R. oligosporus incubation has a high antioxidant content of isoflavones that can regulate oxidative stress in diabetes. In this study, we evaluate the effects of Soyferment-Tempeh on lipid profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) gene expression in type 2 diabetic mice. A total of 30 mice with the age of eight weeks were divided into six groups as follows: A) nondiabetic, B) diabetic mice, C) diabetic mice with metformin, D), E), and F) diabetic mice with Soyferment-Tempeh doses of 10, 20, or 40mg/100g body weight (BW), respectively, were administered treatments orally by gavages. Blood was collected for assessment of blood glucose level, and lipid profile before and after 3 weeks of the administration. After sacrificing the mice, livers were used for RBP4 and PEPCK gene expression assessment. Supplementation with three different doses of Soyferment-Tempeh in streptozotocin-induced diabetic mice for 21 days significantly (p<0.001) reduced blood glucose level, total cholesterol, triglycerides, low-density lipoprotein level, atherogenic index, and increased high-density lipoprotein level. There was a significant decrease in RBP4 gene expression in the Soyferment-Tempeh of dose 10mg/100g BW treatment groups (p<0.05), while the PEPCK gene expression did not significantly differ (p>0.05). These results demonstrate that supplementation with Soyferment-Tempeh decreases blood glucose level, atherogenic index, improves lipid profile, and decreases RBP4.
format	article
author	Prasetyastuti Prasetyastuti Dian Setiawan Ghozali
author_facet	Prasetyastuti Prasetyastuti Dian Setiawan Ghozali
author_sort	Prasetyastuti Prasetyastuti
title	The Effects of Soyferment-Tempeh on Lipid Profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) Gene Expression in Type 2 Diabetic Mice
title_short	The Effects of Soyferment-Tempeh on Lipid Profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) Gene Expression in Type 2 Diabetic Mice
title_full	The Effects of Soyferment-Tempeh on Lipid Profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) Gene Expression in Type 2 Diabetic Mice
title_fullStr	The Effects of Soyferment-Tempeh on Lipid Profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) Gene Expression in Type 2 Diabetic Mice
title_full_unstemmed	The Effects of Soyferment-Tempeh on Lipid Profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) Gene Expression in Type 2 Diabetic Mice
title_sort	effects of soyferment-tempeh on lipid profile, retinol-binding protein 4 (rbp4), and phosphoenolpyruvate carboxykinase (pepck) gene expression in type 2 diabetic mice
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/81037e9b11ba476fa7b687a15cf006c2
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The Effects of Soyferment-Tempeh on Lipid Profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) Gene Expression in Type 2 Diabetic Mice

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