Intramedullary injury combined with osteoporosis therapeutics regulates targeted local osteogenesis

Abstract Bone marrow ablation prompts transient bone formation in nearly the entire medullary cavity before marrow regeneration occurs. Here, we establish a procedure to direct bone formation in a desired particular site within the medullary cavity for support of biomedical devices. Local intramedul...

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Autores principales:	Yoko Miyazaki-Asato, Kiyono Koi, Hiroki Fujimoto, Kae Kakura, Hirofumi Kido, Tsukasa Yanagi, Junro Yamashita
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8107e417efbf4574b3bcd599c86f1d42
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spelling	oai:doaj.org-article:8107e417efbf4574b3bcd599c86f1d422021-12-02T15:23:03ZIntramedullary injury combined with osteoporosis therapeutics regulates targeted local osteogenesis10.1038/s41598-020-80316-y2045-2322https://doaj.org/article/8107e417efbf4574b3bcd599c86f1d422021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80316-yhttps://doaj.org/toc/2045-2322Abstract Bone marrow ablation prompts transient bone formation in nearly the entire medullary cavity before marrow regeneration occurs. Here, we establish a procedure to direct bone formation in a desired particular site within the medullary cavity for support of biomedical devices. Local intramedullary injury was performed in the tibiae of rats and parathyroid hormone (PTH), alendronate, or saline was administered. Newly generated bone in the medulla was assessed by micro-CT and histology. To evaluate the function of newly generated bone, animals received intramedullary injury in tibiae followed by daily PTH. At day-14, implants were placed in the endocortical bone and the bone response to the implants was assessed. The fate of newly generated bone was compared with and without implants. We found that neither intramedullary injury nor medication alone resulted in bone formation. However, when combined, substantial bone was generated locally inside the diaphyseal medulla. Newly formed bone disappeared without implant placement but was retained with implants. Bone was especially retained around and between the implants. This study found that local bone marrow disruption followed by PTH or alendronate generated substantial cancellous bone locally in the diaphyseal medulla. This approach offers promise as a tissue engineering tool in medicine and dentistry.Yoko Miyazaki-AsatoKiyono KoiHiroki FujimotoKae KakuraHirofumi KidoTsukasa YanagiJunro YamashitaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoko Miyazaki-Asato Kiyono Koi Hiroki Fujimoto Kae Kakura Hirofumi Kido Tsukasa Yanagi Junro Yamashita Intramedullary injury combined with osteoporosis therapeutics regulates targeted local osteogenesis
description	Abstract Bone marrow ablation prompts transient bone formation in nearly the entire medullary cavity before marrow regeneration occurs. Here, we establish a procedure to direct bone formation in a desired particular site within the medullary cavity for support of biomedical devices. Local intramedullary injury was performed in the tibiae of rats and parathyroid hormone (PTH), alendronate, or saline was administered. Newly generated bone in the medulla was assessed by micro-CT and histology. To evaluate the function of newly generated bone, animals received intramedullary injury in tibiae followed by daily PTH. At day-14, implants were placed in the endocortical bone and the bone response to the implants was assessed. The fate of newly generated bone was compared with and without implants. We found that neither intramedullary injury nor medication alone resulted in bone formation. However, when combined, substantial bone was generated locally inside the diaphyseal medulla. Newly formed bone disappeared without implant placement but was retained with implants. Bone was especially retained around and between the implants. This study found that local bone marrow disruption followed by PTH or alendronate generated substantial cancellous bone locally in the diaphyseal medulla. This approach offers promise as a tissue engineering tool in medicine and dentistry.
format	article
author	Yoko Miyazaki-Asato Kiyono Koi Hiroki Fujimoto Kae Kakura Hirofumi Kido Tsukasa Yanagi Junro Yamashita
author_facet	Yoko Miyazaki-Asato Kiyono Koi Hiroki Fujimoto Kae Kakura Hirofumi Kido Tsukasa Yanagi Junro Yamashita
author_sort	Yoko Miyazaki-Asato
title	Intramedullary injury combined with osteoporosis therapeutics regulates targeted local osteogenesis
title_short	Intramedullary injury combined with osteoporosis therapeutics regulates targeted local osteogenesis
title_full	Intramedullary injury combined with osteoporosis therapeutics regulates targeted local osteogenesis
title_fullStr	Intramedullary injury combined with osteoporosis therapeutics regulates targeted local osteogenesis
title_full_unstemmed	Intramedullary injury combined with osteoporosis therapeutics regulates targeted local osteogenesis
title_sort	intramedullary injury combined with osteoporosis therapeutics regulates targeted local osteogenesis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8107e417efbf4574b3bcd599c86f1d42
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Intramedullary injury combined with osteoporosis therapeutics regulates targeted local osteogenesis

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