Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification
Z Vivian Feng,1,* Wen Shuo Chen,2,* Khomson Keratithamkul,1 Michael Stoick,1 Brittany Kapala,3 Eryn Johnson,3 An-Chi Huang,2 Ting Yu Chin,4 Yui Whei Chen-Yang,2 Mong-Lin Yang3 1Chemistry Department, Augsburg College, Minneapolis, MN, USA; 2Department of Chemistry, Center for Nanotechnology, Center f...
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Dove Medical Press
2016
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oai:doaj.org-article:81099ec15e3d45bb97b48537e5d3a0cb2021-12-02T00:46:37ZDegradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification1178-2013https://doaj.org/article/81099ec15e3d45bb97b48537e5d3a0cb2016-02-01T00:00:00Zhttps://www.dovepress.com/degradation-of-the-electrospun-silica-nanofiber-in-a-biological-medium-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Z Vivian Feng,1,* Wen Shuo Chen,2,* Khomson Keratithamkul,1 Michael Stoick,1 Brittany Kapala,3 Eryn Johnson,3 An-Chi Huang,2 Ting Yu Chin,4 Yui Whei Chen-Yang,2 Mong-Lin Yang3 1Chemistry Department, Augsburg College, Minneapolis, MN, USA; 2Department of Chemistry, Center for Nanotechnology, Center for Biomedical Technology, Chung Yuan Christian University, Chung Li, Taiwan, Republic of China; 3Department of Science, Concordia University Saint Paul, Saint Paul, MN, USA; 4Department of Bioscience Technology, Chung Yuan Christian University, Chung Li, Taiwan, Republic of China *These authors contributed equally to this work Abstract: In this work, silica nanofibers (SNFs) were prepared by an electrospinning method and modified with poly-D-lysine (PDL) or (3-aminopropyl) trimethoxysilane (APTS) making biocompatible and degradable substrates for neuronal growth. The as-prepared SNF, modified SNF-PDL, and SNF-APTS were evaluated using scanning electron microscopy, nitrogen adsorption/desorption isotherms, contact angle measurements, and inductively coupled plasma atomic emission spectroscopy. Herein, the scanning electron microscopic images revealed that dissolution occurred in a corrosion-like manner by enlarging porous structures, which led to loss of structural integrity. In addition, covalently modified SNF-APTS with more hydrophobic surfaces and smaller surface areas resulted in significantly slower dissolution compared to SNF and physically modified SNF-PDL, revealing that different surface modifications can be used to tune the dissolution rate. Growth of primary hippocampal neuron on all substrates led to a slower dissolution rate. The three-dimensional SNF with larger surface area and higher surface density of the amino group promoted better cell attachment and resulted in an increased neurite density. This is the first known work addressing the degradability of SNF substrate in physiological conditions with neuron growth in vitro, suggesting a strong potential for the applications of the material in controlled drug release. Keywords: silica nanofibers, electrospinning, dissolution, neurite density, surface modificationFeng ZVChen WSKeratithamkul KStoick MKapala BJohnson EHuang ACChin TYChen-Yang YWYang MLDove Medical PressarticleSilica nanofiberselectrospinningdissolutionneurite densitysurface-modificationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 729-741 (2016) |
| institution |
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| collection |
DOAJ |
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EN |
| topic |
Silica nanofibers electrospinning dissolution neurite density surface-modification Medicine (General) R5-920 |
| spellingShingle |
Silica nanofibers electrospinning dissolution neurite density surface-modification Medicine (General) R5-920 Feng ZV Chen WS Keratithamkul K Stoick M Kapala B Johnson E Huang AC Chin TY Chen-Yang YW Yang ML Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
| description |
Z Vivian Feng,1,* Wen Shuo Chen,2,* Khomson Keratithamkul,1 Michael Stoick,1 Brittany Kapala,3 Eryn Johnson,3 An-Chi Huang,2 Ting Yu Chin,4 Yui Whei Chen-Yang,2 Mong-Lin Yang3 1Chemistry Department, Augsburg College, Minneapolis, MN, USA; 2Department of Chemistry, Center for Nanotechnology, Center for Biomedical Technology, Chung Yuan Christian University, Chung Li, Taiwan, Republic of China; 3Department of Science, Concordia University Saint Paul, Saint Paul, MN, USA; 4Department of Bioscience Technology, Chung Yuan Christian University, Chung Li, Taiwan, Republic of China *These authors contributed equally to this work Abstract: In this work, silica nanofibers (SNFs) were prepared by an electrospinning method and modified with poly-D-lysine (PDL) or (3-aminopropyl) trimethoxysilane (APTS) making biocompatible and degradable substrates for neuronal growth. The as-prepared SNF, modified SNF-PDL, and SNF-APTS were evaluated using scanning electron microscopy, nitrogen adsorption/desorption isotherms, contact angle measurements, and inductively coupled plasma atomic emission spectroscopy. Herein, the scanning electron microscopic images revealed that dissolution occurred in a corrosion-like manner by enlarging porous structures, which led to loss of structural integrity. In addition, covalently modified SNF-APTS with more hydrophobic surfaces and smaller surface areas resulted in significantly slower dissolution compared to SNF and physically modified SNF-PDL, revealing that different surface modifications can be used to tune the dissolution rate. Growth of primary hippocampal neuron on all substrates led to a slower dissolution rate. The three-dimensional SNF with larger surface area and higher surface density of the amino group promoted better cell attachment and resulted in an increased neurite density. This is the first known work addressing the degradability of SNF substrate in physiological conditions with neuron growth in vitro, suggesting a strong potential for the applications of the material in controlled drug release. Keywords: silica nanofibers, electrospinning, dissolution, neurite density, surface modification |
| format |
article |
| author |
Feng ZV Chen WS Keratithamkul K Stoick M Kapala B Johnson E Huang AC Chin TY Chen-Yang YW Yang ML |
| author_facet |
Feng ZV Chen WS Keratithamkul K Stoick M Kapala B Johnson E Huang AC Chin TY Chen-Yang YW Yang ML |
| author_sort |
Feng ZV |
| title |
Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
| title_short |
Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
| title_full |
Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
| title_fullStr |
Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
| title_full_unstemmed |
Degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
| title_sort |
degradation of the electrospun silica nanofiber in a biological medium for primary hippocampal neuron – effect of surface modification |
| publisher |
Dove Medical Press |
| publishDate |
2016 |
| url |
https://doaj.org/article/81099ec15e3d45bb97b48537e5d3a0cb |
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