Ferroptosis is Involved in Hyperoxic Lung Injury in Neonatal Rats

Danyun Jia,1 Jinyu Zheng,2 Yiyang Zhou,2 Jinqiu Jia,3 Xiaoxiao Ye,4 Bingbing Zhou,4 Xingxing Chen,4 Yunchang Mo,1 Junlu Wang1 1Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, People’s Republic of China; 2Department of Clinical Med...

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Autores principales:	Jia D, Zheng J, Zhou Y, Jia J, Ye X, Zhou B, Chen X, Mo Y, Wang J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	ferroptosis hyperoxic lung injury neonatal rats Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/810a554fd1294fa0b199b69561c5db4a
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spelling	oai:doaj.org-article:810a554fd1294fa0b199b69561c5db4a2021-12-02T17:57:03ZFerroptosis is Involved in Hyperoxic Lung Injury in Neonatal Rats1178-7031https://doaj.org/article/810a554fd1294fa0b199b69561c5db4a2021-10-01T00:00:00Zhttps://www.dovepress.com/ferroptosis-is-involved-in-hyperoxic-lung-injury-in-neonatal-rats-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Danyun Jia,1 Jinyu Zheng,2 Yiyang Zhou,2 Jinqiu Jia,3 Xiaoxiao Ye,4 Bingbing Zhou,4 Xingxing Chen,4 Yunchang Mo,1 Junlu Wang1 1Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, People’s Republic of China; 2Department of Clinical Medicine, Wenzhou Medical University, Wenzhou, 325000, Zhejiang, People’s Republic of China; 3Department of Pediatric, Taizhou Women and Children’s Hospital of Wenzhou Medical University, Taizhou, 317599, Zhejiang, People’s Republic of China; 4Department of Nursing, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, People’s Republic of ChinaCorrespondence: Yunchang Mo; Junlu Wang Email myc1104@163.com; wjlprof2020@163.comPurpose: To evaluate whether ferroptosis is involved in hyperoxic acute lung injury (HALI) and its mechanisms through the HALI model.Methods: HE staining was used to assess lung injury pathology after the establishment of neonatal rat HALI model. ELISA was used to detect ROS, GPX4, and GSH expression. Prussian blue staining and Western Blot were used to detect iron deposition and the expression of ferroptosis-related proteins, respectively.Results: The HALI group showed pathological changes with larger and fewer alveoli and thicker alveolar septa after HE staining. Prussian blue staining detected significant iron deposition in the lung tissue of the HALI group. GPX4, GSH, GSS, and SLC7A11 expressions were significantly decreased in the HALI group than in the normal control group. In contrast, ROS, TFRC, FHC, and FLC expressions showed opposite results (p< 0.05).Conclusion: Ferroptosis may be involved in the pathological process of hyperoxic lung injury in neonatal rats.Keywords: ferroptosis, hyperoxic lung injury, neonatal ratsJia DZheng JZhou YJia JYe XZhou BChen XMo YWang JDove Medical Pressarticleferroptosishyperoxic lung injuryneonatal ratsPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 5393-5401 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ferroptosis hyperoxic lung injury neonatal rats Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	ferroptosis hyperoxic lung injury neonatal rats Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Jia D Zheng J Zhou Y Jia J Ye X Zhou B Chen X Mo Y Wang J Ferroptosis is Involved in Hyperoxic Lung Injury in Neonatal Rats
description	Danyun Jia,1 Jinyu Zheng,2 Yiyang Zhou,2 Jinqiu Jia,3 Xiaoxiao Ye,4 Bingbing Zhou,4 Xingxing Chen,4 Yunchang Mo,1 Junlu Wang1 1Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, People’s Republic of China; 2Department of Clinical Medicine, Wenzhou Medical University, Wenzhou, 325000, Zhejiang, People’s Republic of China; 3Department of Pediatric, Taizhou Women and Children’s Hospital of Wenzhou Medical University, Taizhou, 317599, Zhejiang, People’s Republic of China; 4Department of Nursing, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, People’s Republic of ChinaCorrespondence: Yunchang Mo; Junlu Wang Email myc1104@163.com; wjlprof2020@163.comPurpose: To evaluate whether ferroptosis is involved in hyperoxic acute lung injury (HALI) and its mechanisms through the HALI model.Methods: HE staining was used to assess lung injury pathology after the establishment of neonatal rat HALI model. ELISA was used to detect ROS, GPX4, and GSH expression. Prussian blue staining and Western Blot were used to detect iron deposition and the expression of ferroptosis-related proteins, respectively.Results: The HALI group showed pathological changes with larger and fewer alveoli and thicker alveolar septa after HE staining. Prussian blue staining detected significant iron deposition in the lung tissue of the HALI group. GPX4, GSH, GSS, and SLC7A11 expressions were significantly decreased in the HALI group than in the normal control group. In contrast, ROS, TFRC, FHC, and FLC expressions showed opposite results (p< 0.05).Conclusion: Ferroptosis may be involved in the pathological process of hyperoxic lung injury in neonatal rats.Keywords: ferroptosis, hyperoxic lung injury, neonatal rats
format	article
author	Jia D Zheng J Zhou Y Jia J Ye X Zhou B Chen X Mo Y Wang J
author_facet	Jia D Zheng J Zhou Y Jia J Ye X Zhou B Chen X Mo Y Wang J
author_sort	Jia D
title	Ferroptosis is Involved in Hyperoxic Lung Injury in Neonatal Rats
title_short	Ferroptosis is Involved in Hyperoxic Lung Injury in Neonatal Rats
title_full	Ferroptosis is Involved in Hyperoxic Lung Injury in Neonatal Rats
title_fullStr	Ferroptosis is Involved in Hyperoxic Lung Injury in Neonatal Rats
title_full_unstemmed	Ferroptosis is Involved in Hyperoxic Lung Injury in Neonatal Rats
title_sort	ferroptosis is involved in hyperoxic lung injury in neonatal rats
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/810a554fd1294fa0b199b69561c5db4a
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_version_	1718379055613476864

Ferroptosis is Involved in Hyperoxic Lung Injury in Neonatal Rats

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