The Role of Estrogen Receptor α in Response to Longitudinal Bone Growth in ob/ob Mice

The absence of leptin results in contrasting growth pattern of appendicular and axial bone growth in ob/ob mice. Endochondral bone formation is an important procedure of growth plate determining the bone growth, where this procedure is also regulated by estrogen and its receptor (ER) signaling pathw...

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Autores principales:	Lin-Yu Jin, Chen Guo, Shuai Xu, Hai-Ying Liu, Xin-Feng Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	estrogen receptor leptin skeletal longitudinal growth growth plate endochondral bone formation Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/810a6743f3fa467dbd1740b23b6ffc5d
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spelling	oai:doaj.org-article:810a6743f3fa467dbd1740b23b6ffc5d2021-12-01T22:55:52ZThe Role of Estrogen Receptor α in Response to Longitudinal Bone Growth in ob/ob Mice1664-239210.3389/fendo.2021.749449https://doaj.org/article/810a6743f3fa467dbd1740b23b6ffc5d2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fendo.2021.749449/fullhttps://doaj.org/toc/1664-2392The absence of leptin results in contrasting growth pattern of appendicular and axial bone growth in ob/ob mice. Endochondral bone formation is an important procedure of growth plate determining the bone growth, where this procedure is also regulated by estrogen and its receptor (ER) signaling pathway. The present study is undertaken to explore the roles of ERs in regulating the different growth patterns in ob/ob mice. In this study, C57BL/6 female mice were used as wild-type (WT) mice; ob/ob mice and WT mice were age-matched fed, and bone length is analyzed by X-ray plain film at the 12 weeks old. We confirm that ob/ob mice have shorter femoral length and longer spine length than WT mice (p < 0.05). The contrasting expression patterns of chondrocyte proliferation proteins and hypertrophic marker proteins are also observed from the femur and spinal growth plate of ob/ob mice compared with WT mice (p < 0.01). Spearman’s analysis showed that body length (axial and appendicular length) is positively related to the expression level of ERα in growth plate. Three-week-old female ob/ob mice are randomized divided into three groups: 1) ob/ob + ctrl, 2) ob/ob + ERα antagonist (MPP), and 3) ob/ob + ERβ antagonist (PHTPP). Age-matched C57BL/6 mice were also divided into three groups, same as the groups of ob/ob mice. MPP and PHTPP were administered by intraperitoneal injection for 6 weeks. However, the results of X-ray and H&E staining demonstrate that leptin deficiency seems to disturb the regulating effects of ER antagonists on longitudinal bone growth. These findings suggested that region-specific expression of ERα might be associated with contrasting phenotypes of axial and appendicular bone growth in ob/ob mice. However, ER signaling on longitudinal bone growth was blunted by leptin deficiency in ob/ob mice, and the underlying association between ERs and leptin needs to be explored in future work.Lin-Yu JinChen GuoShuai XuHai-Ying LiuXin-Feng LiFrontiers Media S.A.articleestrogen receptorleptinskeletal longitudinal growthgrowth plateendochondral bone formationDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENFrontiers in Endocrinology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	estrogen receptor leptin skeletal longitudinal growth growth plate endochondral bone formation Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	estrogen receptor leptin skeletal longitudinal growth growth plate endochondral bone formation Diseases of the endocrine glands. Clinical endocrinology RC648-665 Lin-Yu Jin Chen Guo Shuai Xu Hai-Ying Liu Xin-Feng Li The Role of Estrogen Receptor α in Response to Longitudinal Bone Growth in ob/ob Mice
description	The absence of leptin results in contrasting growth pattern of appendicular and axial bone growth in ob/ob mice. Endochondral bone formation is an important procedure of growth plate determining the bone growth, where this procedure is also regulated by estrogen and its receptor (ER) signaling pathway. The present study is undertaken to explore the roles of ERs in regulating the different growth patterns in ob/ob mice. In this study, C57BL/6 female mice were used as wild-type (WT) mice; ob/ob mice and WT mice were age-matched fed, and bone length is analyzed by X-ray plain film at the 12 weeks old. We confirm that ob/ob mice have shorter femoral length and longer spine length than WT mice (p < 0.05). The contrasting expression patterns of chondrocyte proliferation proteins and hypertrophic marker proteins are also observed from the femur and spinal growth plate of ob/ob mice compared with WT mice (p < 0.01). Spearman’s analysis showed that body length (axial and appendicular length) is positively related to the expression level of ERα in growth plate. Three-week-old female ob/ob mice are randomized divided into three groups: 1) ob/ob + ctrl, 2) ob/ob + ERα antagonist (MPP), and 3) ob/ob + ERβ antagonist (PHTPP). Age-matched C57BL/6 mice were also divided into three groups, same as the groups of ob/ob mice. MPP and PHTPP were administered by intraperitoneal injection for 6 weeks. However, the results of X-ray and H&E staining demonstrate that leptin deficiency seems to disturb the regulating effects of ER antagonists on longitudinal bone growth. These findings suggested that region-specific expression of ERα might be associated with contrasting phenotypes of axial and appendicular bone growth in ob/ob mice. However, ER signaling on longitudinal bone growth was blunted by leptin deficiency in ob/ob mice, and the underlying association between ERs and leptin needs to be explored in future work.
format	article
author	Lin-Yu Jin Chen Guo Shuai Xu Hai-Ying Liu Xin-Feng Li
author_facet	Lin-Yu Jin Chen Guo Shuai Xu Hai-Ying Liu Xin-Feng Li
author_sort	Lin-Yu Jin
title	The Role of Estrogen Receptor α in Response to Longitudinal Bone Growth in ob/ob Mice
title_short	The Role of Estrogen Receptor α in Response to Longitudinal Bone Growth in ob/ob Mice
title_full	The Role of Estrogen Receptor α in Response to Longitudinal Bone Growth in ob/ob Mice
title_fullStr	The Role of Estrogen Receptor α in Response to Longitudinal Bone Growth in ob/ob Mice
title_full_unstemmed	The Role of Estrogen Receptor α in Response to Longitudinal Bone Growth in ob/ob Mice
title_sort	role of estrogen receptor α in response to longitudinal bone growth in ob/ob mice
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/810a6743f3fa467dbd1740b23b6ffc5d
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The Role of Estrogen Receptor α in Response to Longitudinal Bone Growth in ob/ob Mice

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