Citrus paradisi and Citrus reticulata essential oils interfere with Pseudomonas aeruginosa quorum sensing in vivo on Caenorhabditis elegans

ABSTRACT: Background: Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen, considered a leading cause of acute and chronic infections in immunocompromised patients. P. aeruginosa uses quorum sensing to control virulence and biofilm formation. To combat this human-resistant pathogen incr...

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Autores principales:	Romina E. D'Almeida, Nahir Sued, Mario E. Arena
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Pseudomonas aeruginosa Caenorhabditis elegans Citrus paradisi Citrus reticulata Essential oils Quorum sensing Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/810bdee073fb4845a14dcae3937a7b85
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spelling	oai:doaj.org-article:810bdee073fb4845a14dcae3937a7b852021-11-20T05:15:56ZCitrus paradisi and Citrus reticulata essential oils interfere with Pseudomonas aeruginosa quorum sensing in vivo on Caenorhabditis elegans2667-031310.1016/j.phyplu.2021.100160https://doaj.org/article/810bdee073fb4845a14dcae3937a7b852022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667031321001421https://doaj.org/toc/2667-0313ABSTRACT: Background: Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen, considered a leading cause of acute and chronic infections in immunocompromised patients. P. aeruginosa uses quorum sensing to control virulence and biofilm formation. To combat this human-resistant pathogen increasing attention has been paid to anti-QS compounds from natural products as potential therapeutic agents.Purpose: To assess the efficacy of C. paradisi (Grapefruit) and C. reticulata (Mandarin) commercial EOs (obtained by cold-pressing and cold-pressing followed by steam distillation, named EOP and EOPD, respectively) and their majority component, limonene, in inhibiting the effect of QS-controlled virulence factors of P. aeruginosa PAO1 and PA14 in a C. elegans infection model.Results: C. paradisi and C. reticulata EOs at 0.125% (v/v) significantly inhibited the in vitro biofilm formation of PAO1 and PA14 strains between 40 and 50%. This EOs concentration, safe for C. elegans according to the survival and brood size assays, rescued the nematodes from P. aeruginosa infections, reducing their death rate by 20–30% in the paralysis assay, and increasing worm lifespan with median survivals of 6 (Grapefruit) and 5 (Mandarin) in the slow killing assay (3 for non-treated worms). Limonene protected nematodes from death in these assays although less effectively than both EOs species. Grapefruit EOs rescued the nematodes in 45–50% from phenazine-death, while mandarin EOs and limonene rescued them in 30%. In the food choice assay, worms preferred the PA14 grown with 0.125% of mandarin and grapefruit EOs or limonene after 3-4 hours (CI=0.11 to 0.18).Conclusion: Our results suggest that C. paradisi and C. reticulata EOPDs, with lower commercial value but similar in vivo effects than EOPs, are sources of anti-QS agents for controlling P. aeruginosa infections, therefore should be considered as potential candidates for the development of novel therapeutics against persistent microorganisms.Romina E. D'AlmeidaNahir SuedMario E. ArenaElsevierarticlePseudomonas aeruginosaCaenorhabditis elegansCitrus paradisiCitrus reticulataEssential oilsQuorum sensingOther systems of medicineRZ201-999ENPhytomedicine Plus, Vol 2, Iss 1, Pp 100160- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pseudomonas aeruginosa Caenorhabditis elegans Citrus paradisi Citrus reticulata Essential oils Quorum sensing Other systems of medicine RZ201-999
spellingShingle	Pseudomonas aeruginosa Caenorhabditis elegans Citrus paradisi Citrus reticulata Essential oils Quorum sensing Other systems of medicine RZ201-999 Romina E. D'Almeida Nahir Sued Mario E. Arena Citrus paradisi and Citrus reticulata essential oils interfere with Pseudomonas aeruginosa quorum sensing in vivo on Caenorhabditis elegans
description	ABSTRACT: Background: Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen, considered a leading cause of acute and chronic infections in immunocompromised patients. P. aeruginosa uses quorum sensing to control virulence and biofilm formation. To combat this human-resistant pathogen increasing attention has been paid to anti-QS compounds from natural products as potential therapeutic agents.Purpose: To assess the efficacy of C. paradisi (Grapefruit) and C. reticulata (Mandarin) commercial EOs (obtained by cold-pressing and cold-pressing followed by steam distillation, named EOP and EOPD, respectively) and their majority component, limonene, in inhibiting the effect of QS-controlled virulence factors of P. aeruginosa PAO1 and PA14 in a C. elegans infection model.Results: C. paradisi and C. reticulata EOs at 0.125% (v/v) significantly inhibited the in vitro biofilm formation of PAO1 and PA14 strains between 40 and 50%. This EOs concentration, safe for C. elegans according to the survival and brood size assays, rescued the nematodes from P. aeruginosa infections, reducing their death rate by 20–30% in the paralysis assay, and increasing worm lifespan with median survivals of 6 (Grapefruit) and 5 (Mandarin) in the slow killing assay (3 for non-treated worms). Limonene protected nematodes from death in these assays although less effectively than both EOs species. Grapefruit EOs rescued the nematodes in 45–50% from phenazine-death, while mandarin EOs and limonene rescued them in 30%. In the food choice assay, worms preferred the PA14 grown with 0.125% of mandarin and grapefruit EOs or limonene after 3-4 hours (CI=0.11 to 0.18).Conclusion: Our results suggest that C. paradisi and C. reticulata EOPDs, with lower commercial value but similar in vivo effects than EOPs, are sources of anti-QS agents for controlling P. aeruginosa infections, therefore should be considered as potential candidates for the development of novel therapeutics against persistent microorganisms.
format	article
author	Romina E. D'Almeida Nahir Sued Mario E. Arena
author_facet	Romina E. D'Almeida Nahir Sued Mario E. Arena
author_sort	Romina E. D'Almeida
title	Citrus paradisi and Citrus reticulata essential oils interfere with Pseudomonas aeruginosa quorum sensing in vivo on Caenorhabditis elegans
title_short	Citrus paradisi and Citrus reticulata essential oils interfere with Pseudomonas aeruginosa quorum sensing in vivo on Caenorhabditis elegans
title_full	Citrus paradisi and Citrus reticulata essential oils interfere with Pseudomonas aeruginosa quorum sensing in vivo on Caenorhabditis elegans
title_fullStr	Citrus paradisi and Citrus reticulata essential oils interfere with Pseudomonas aeruginosa quorum sensing in vivo on Caenorhabditis elegans
title_full_unstemmed	Citrus paradisi and Citrus reticulata essential oils interfere with Pseudomonas aeruginosa quorum sensing in vivo on Caenorhabditis elegans
title_sort	citrus paradisi and citrus reticulata essential oils interfere with pseudomonas aeruginosa quorum sensing in vivo on caenorhabditis elegans
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/810bdee073fb4845a14dcae3937a7b85
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Citrus paradisi and Citrus reticulata essential oils interfere with Pseudomonas aeruginosa quorum sensing in vivo on Caenorhabditis elegans

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