Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures

Abstract Models of bone remodelling could be useful in drug discovery, particularly if the model is one that replicates bone regeneration with reduction in osteoclast activity. Here we use nanovibrational stimulation to achieve this in a 3D co-culture of primary human osteoprogenitor and osteoclast...

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Autores principales:	John W. Kennedy, P. Monica Tsimbouri, Paul Campsie, Shatakshi Sood, Peter G. Childs, Stuart Reid, Peter S. Young, Dominic R. M. Meek, Carl S. Goodyear, Matthew J. Dalby
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/810f83f7a49e40a688c667b930757c1d
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spelling	oai:doaj.org-article:810f83f7a49e40a688c667b930757c1d2021-11-28T12:21:31ZNanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures10.1038/s41598-021-02139-92045-2322https://doaj.org/article/810f83f7a49e40a688c667b930757c1d2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02139-9https://doaj.org/toc/2045-2322Abstract Models of bone remodelling could be useful in drug discovery, particularly if the model is one that replicates bone regeneration with reduction in osteoclast activity. Here we use nanovibrational stimulation to achieve this in a 3D co-culture of primary human osteoprogenitor and osteoclast progenitor cells. We show that 1000 Hz frequency, 40 nm amplitude vibration reduces osteoclast formation and activity in human mononuclear CD14+ blood cells. Additionally, this nanoscale vibration both enhances osteogenesis and reduces osteoclastogenesis in a co-culture of primary human bone marrow stromal cells and bone marrow hematopoietic cells. Further, we use metabolomics to identify Akt (protein kinase C) as a potential mediator. Akt is known to be involved in bone differentiation via transforming growth factor beta 1 (TGFβ1) and bone morphogenetic protein 2 (BMP2) and it has been implicated in reduced osteoclast activity via Guanine nucleotide-binding protein subunit α13 (Gα13). With further validation, our nanovibrational bioreactor could be used to help provide humanised 3D models for drug screening.John W. KennedyP. Monica TsimbouriPaul CampsieShatakshi SoodPeter G. ChildsStuart ReidPeter S. YoungDominic R. M. MeekCarl S. GoodyearMatthew J. DalbyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q John W. Kennedy P. Monica Tsimbouri Paul Campsie Shatakshi Sood Peter G. Childs Stuart Reid Peter S. Young Dominic R. M. Meek Carl S. Goodyear Matthew J. Dalby Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures
description	Abstract Models of bone remodelling could be useful in drug discovery, particularly if the model is one that replicates bone regeneration with reduction in osteoclast activity. Here we use nanovibrational stimulation to achieve this in a 3D co-culture of primary human osteoprogenitor and osteoclast progenitor cells. We show that 1000 Hz frequency, 40 nm amplitude vibration reduces osteoclast formation and activity in human mononuclear CD14+ blood cells. Additionally, this nanoscale vibration both enhances osteogenesis and reduces osteoclastogenesis in a co-culture of primary human bone marrow stromal cells and bone marrow hematopoietic cells. Further, we use metabolomics to identify Akt (protein kinase C) as a potential mediator. Akt is known to be involved in bone differentiation via transforming growth factor beta 1 (TGFβ1) and bone morphogenetic protein 2 (BMP2) and it has been implicated in reduced osteoclast activity via Guanine nucleotide-binding protein subunit α13 (Gα13). With further validation, our nanovibrational bioreactor could be used to help provide humanised 3D models for drug screening.
format	article
author	John W. Kennedy P. Monica Tsimbouri Paul Campsie Shatakshi Sood Peter G. Childs Stuart Reid Peter S. Young Dominic R. M. Meek Carl S. Goodyear Matthew J. Dalby
author_facet	John W. Kennedy P. Monica Tsimbouri Paul Campsie Shatakshi Sood Peter G. Childs Stuart Reid Peter S. Young Dominic R. M. Meek Carl S. Goodyear Matthew J. Dalby
author_sort	John W. Kennedy
title	Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures
title_short	Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures
title_full	Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures
title_fullStr	Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures
title_full_unstemmed	Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures
title_sort	nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/810f83f7a49e40a688c667b930757c1d
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Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures

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