Adhesion Properties of Lactobacillus plantarum Dad-13 and Lactobacillus plantarum Mut-7 on Sprague Dawley Rat Intestine

Adhesion capacity is considered one of the selection criteria for probiotic strains. The purpose of this study was to determine the adhesion properties of two candidate probiotics, Lactobacillus plantarum Dad-13 and Lactobacillus plantarum Mut-7. The evaluation...

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Autores principales:	Arum Darmastuti, Pratama N. Hasan, Rachma Wikandari, Tyas Utami, Endang S. Rahayu, Dian Anggraini Suroto
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	probiotic <i>Lactobacillus plantarum</i> hydrophobicity autoaggregation adhesion ability Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/81113978e5bd4f02afb18ce34d39a94d
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spelling	oai:doaj.org-article:81113978e5bd4f02afb18ce34d39a94d2021-11-25T18:25:14ZAdhesion Properties of <i>Lactobacillus plantarum</i> Dad-13 and <i>Lactobacillus plantarum</i> Mut-7 on Sprague Dawley Rat Intestine10.3390/microorganisms91123362076-2607https://doaj.org/article/81113978e5bd4f02afb18ce34d39a94d2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2336https://doaj.org/toc/2076-2607Adhesion capacity is considered one of the selection criteria for probiotic strains. The purpose of this study was to determine the adhesion properties of two candidate probiotics, <i>Lactobacillus plantarum</i> Dad-13 and <i>Lactobacillus plantarum</i> Mut-7. The evaluation included the hydrophobicity of the cell surface using microbial adhesion to hydrocarbons (MATH), autoaggregation, and the adhesion of <i>L. plantarum</i> Dad-13 and <i>L. plantarum</i> Mut-7 to the intestinal mucosa of Sprague Dawley rat, followed by genomic analysis of the two <i>L. plantarum</i> strains. <i>L. plantarum</i> Dad-13 and <i>L. plantarum</i> Mut-7 showed a high surface hydrophobicity (78.9% and 83.5%) and medium autoaggregation ability (40.9% and 57.5%, respectively). The exposure of both isolates to the surface of the rat intestine increased the total number of lactic acid bacteria on the colon compartment, from 2.9 log CFU/cm<sup>2</sup> to 4.4 log CFU/cm<sup>2</sup> in <i>L. plantarum</i> Dad-13 treatment and to 3.86 log CFU/cm<sup>2</sup> in <i>L. plantarum</i> Mut-7 treatment. The results indicate the ability of two <i>L. plantarum</i> to attach to the surface of the rat intestine. The number of indigenous <i>E. coli</i> in the colon also decreased when the compartment was exposed to <i>L. plantarum</i> Dad-13 and Mut-7, from 2.9 log CFU/cm<sup>2</sup> to 1 log CFU/cm<sup>2</sup>. Genomic analysis revealed that both strains have genes related to adhesion properties that could play an important role in increasing the adherence of probiotics to the intestinal mucosa such as gene encoding fibronectin-binding protein, chaperonin heat shock protein 33 (Hsp33)<b>,</b> and genes related to the capsule and cell wall biosynthesis. Based on these findings, we believe that <i>L. plantarum</i> Dad-13 and <i>L. plantarum</i> Mut-7 have adhesion properties to the intestinal mucosa in the rat intestine model system. The present research will be essential to elucidate the molecular mechanism associated with adhesion in our two probiotic strains.Arum DarmastutiPratama N. HasanRachma WikandariTyas UtamiEndang S. RahayuDian Anggraini SurotoMDPI AGarticleprobiotic<i>Lactobacillus plantarum</i>hydrophobicityautoaggregationadhesion abilityBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2336, p 2336 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	probiotic <i>Lactobacillus plantarum</i> hydrophobicity autoaggregation adhesion ability Biology (General) QH301-705.5
spellingShingle	probiotic <i>Lactobacillus plantarum</i> hydrophobicity autoaggregation adhesion ability Biology (General) QH301-705.5 Arum Darmastuti Pratama N. Hasan Rachma Wikandari Tyas Utami Endang S. Rahayu Dian Anggraini Suroto Adhesion Properties of <i>Lactobacillus plantarum</i> Dad-13 and <i>Lactobacillus plantarum</i> Mut-7 on Sprague Dawley Rat Intestine
description	Adhesion capacity is considered one of the selection criteria for probiotic strains. The purpose of this study was to determine the adhesion properties of two candidate probiotics, <i>Lactobacillus plantarum</i> Dad-13 and <i>Lactobacillus plantarum</i> Mut-7. The evaluation included the hydrophobicity of the cell surface using microbial adhesion to hydrocarbons (MATH), autoaggregation, and the adhesion of <i>L. plantarum</i> Dad-13 and <i>L. plantarum</i> Mut-7 to the intestinal mucosa of Sprague Dawley rat, followed by genomic analysis of the two <i>L. plantarum</i> strains. <i>L. plantarum</i> Dad-13 and <i>L. plantarum</i> Mut-7 showed a high surface hydrophobicity (78.9% and 83.5%) and medium autoaggregation ability (40.9% and 57.5%, respectively). The exposure of both isolates to the surface of the rat intestine increased the total number of lactic acid bacteria on the colon compartment, from 2.9 log CFU/cm<sup>2</sup> to 4.4 log CFU/cm<sup>2</sup> in <i>L. plantarum</i> Dad-13 treatment and to 3.86 log CFU/cm<sup>2</sup> in <i>L. plantarum</i> Mut-7 treatment. The results indicate the ability of two <i>L. plantarum</i> to attach to the surface of the rat intestine. The number of indigenous <i>E. coli</i> in the colon also decreased when the compartment was exposed to <i>L. plantarum</i> Dad-13 and Mut-7, from 2.9 log CFU/cm<sup>2</sup> to 1 log CFU/cm<sup>2</sup>. Genomic analysis revealed that both strains have genes related to adhesion properties that could play an important role in increasing the adherence of probiotics to the intestinal mucosa such as gene encoding fibronectin-binding protein, chaperonin heat shock protein 33 (Hsp33)<b>,</b> and genes related to the capsule and cell wall biosynthesis. Based on these findings, we believe that <i>L. plantarum</i> Dad-13 and <i>L. plantarum</i> Mut-7 have adhesion properties to the intestinal mucosa in the rat intestine model system. The present research will be essential to elucidate the molecular mechanism associated with adhesion in our two probiotic strains.
format	article
author	Arum Darmastuti Pratama N. Hasan Rachma Wikandari Tyas Utami Endang S. Rahayu Dian Anggraini Suroto
author_facet	Arum Darmastuti Pratama N. Hasan Rachma Wikandari Tyas Utami Endang S. Rahayu Dian Anggraini Suroto
author_sort	Arum Darmastuti
title	Adhesion Properties of <i>Lactobacillus plantarum</i> Dad-13 and <i>Lactobacillus plantarum</i> Mut-7 on Sprague Dawley Rat Intestine
title_short	Adhesion Properties of <i>Lactobacillus plantarum</i> Dad-13 and <i>Lactobacillus plantarum</i> Mut-7 on Sprague Dawley Rat Intestine
title_full	Adhesion Properties of <i>Lactobacillus plantarum</i> Dad-13 and <i>Lactobacillus plantarum</i> Mut-7 on Sprague Dawley Rat Intestine
title_fullStr	Adhesion Properties of <i>Lactobacillus plantarum</i> Dad-13 and <i>Lactobacillus plantarum</i> Mut-7 on Sprague Dawley Rat Intestine
title_full_unstemmed	Adhesion Properties of <i>Lactobacillus plantarum</i> Dad-13 and <i>Lactobacillus plantarum</i> Mut-7 on Sprague Dawley Rat Intestine
title_sort	adhesion properties of <i>lactobacillus plantarum</i> dad-13 and <i>lactobacillus plantarum</i> mut-7 on sprague dawley rat intestine
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/81113978e5bd4f02afb18ce34d39a94d
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Adhesion Properties of <i>Lactobacillus plantarum</i> Dad-13 and <i>Lactobacillus plantarum</i> Mut-7 on Sprague Dawley Rat Intestine

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