Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation

Abstract A major challenge in regenerative medicine is to improve therapeutic cells’ delivery and targeting using an efficient and simple protocol. Mesenchymal stem cells (MSC) are currently employed for the treatment of inflammatory-based diseases, due to their powerful immunosoppressive potential....

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Autores principales:	Bruna Corradetti, Francesca Taraballi, Jonathan O. Martinez, Silvia Minardi, Nupur Basu, Guillermo Bauza, Michael Evangelopoulos, Sebastian Powell, Claudia Corbo, Ennio Tasciotti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/811abc51292e44f1a6f41506592aad46
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spelling	oai:doaj.org-article:811abc51292e44f1a6f41506592aad462021-12-02T12:30:12ZHyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation10.1038/s41598-017-08687-32045-2322https://doaj.org/article/811abc51292e44f1a6f41506592aad462017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08687-3https://doaj.org/toc/2045-2322Abstract A major challenge in regenerative medicine is to improve therapeutic cells’ delivery and targeting using an efficient and simple protocol. Mesenchymal stem cells (MSC) are currently employed for the treatment of inflammatory-based diseases, due to their powerful immunosoppressive potential. Here we report a simple and versatile method to transiently overexpress the hyaluronic acid (HA) receptor, CD44, on MSC membranes, to improve their homing potential towards an inflammatory site without affecting their behavior. The effect of HA-coatings on murine MSC was functionally determined both, in vitro and in vivo as a consequence of the transient CD44 overexpression induced by HA. Data obtained from the in vitro migration assay demonstrated a two-fold increase in the migratory potential of HA-treated MSC compared to untreated cells. In an LPS-induced inflamed ear murine model, HA-treated MSC demonstrated a significantly higher inflammatory targeting as observed at 72 hrs as compared to untreated cells. This increased accumulation for HA-treated MSC yielded a substantial reduction in inflammation as demonstrated by the decrease in the expression of pro-inflammatory markers and by the induction of a pro-regenerative environment.Bruna CorradettiFrancesca TaraballiJonathan O. MartinezSilvia MinardiNupur BasuGuillermo BauzaMichael EvangelopoulosSebastian PowellClaudia CorboEnnio TasciottiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bruna Corradetti Francesca Taraballi Jonathan O. Martinez Silvia Minardi Nupur Basu Guillermo Bauza Michael Evangelopoulos Sebastian Powell Claudia Corbo Ennio Tasciotti Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation
description	Abstract A major challenge in regenerative medicine is to improve therapeutic cells’ delivery and targeting using an efficient and simple protocol. Mesenchymal stem cells (MSC) are currently employed for the treatment of inflammatory-based diseases, due to their powerful immunosoppressive potential. Here we report a simple and versatile method to transiently overexpress the hyaluronic acid (HA) receptor, CD44, on MSC membranes, to improve their homing potential towards an inflammatory site without affecting their behavior. The effect of HA-coatings on murine MSC was functionally determined both, in vitro and in vivo as a consequence of the transient CD44 overexpression induced by HA. Data obtained from the in vitro migration assay demonstrated a two-fold increase in the migratory potential of HA-treated MSC compared to untreated cells. In an LPS-induced inflamed ear murine model, HA-treated MSC demonstrated a significantly higher inflammatory targeting as observed at 72 hrs as compared to untreated cells. This increased accumulation for HA-treated MSC yielded a substantial reduction in inflammation as demonstrated by the decrease in the expression of pro-inflammatory markers and by the induction of a pro-regenerative environment.
format	article
author	Bruna Corradetti Francesca Taraballi Jonathan O. Martinez Silvia Minardi Nupur Basu Guillermo Bauza Michael Evangelopoulos Sebastian Powell Claudia Corbo Ennio Tasciotti
author_facet	Bruna Corradetti Francesca Taraballi Jonathan O. Martinez Silvia Minardi Nupur Basu Guillermo Bauza Michael Evangelopoulos Sebastian Powell Claudia Corbo Ennio Tasciotti
author_sort	Bruna Corradetti
title	Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation
title_short	Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation
title_full	Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation
title_fullStr	Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation
title_full_unstemmed	Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation
title_sort	hyaluronic acid coatings as a simple and efficient approach to improve msc homing toward the site of inflammation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/811abc51292e44f1a6f41506592aad46
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Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation

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