Bacteriocin-mediated inhibition of some common pathogens by wild and mutant Lactobacillus species and in vitro amplification of bacteriocin encoding genes

Lactobacilli are the most common probiotics used in food and other industries because of their capability of producing bacteriocins. Bacteriocins are compounds that are used to kill pathogenic microorganisms. As most bacteria have become resistant to synthetic antibacterial tools, the importance of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Arooj Ahsan, Bushra Mazhar, Muhammad Kamran Khan, Madiha Mustafa, Muhammad Hammad, Nazish Mazhar Ali
Formato:	article
Lenguaje:	EN
Publicado:	International Association of Physical Chemists (IAPC) 2021
Materias:	Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/811c6f0cbe2843cb93b52ba3c1b732db
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:811c6f0cbe2843cb93b52ba3c1b732db
record_format	dspace
spelling	oai:doaj.org-article:811c6f0cbe2843cb93b52ba3c1b732db2021-11-15T10:03:41ZBacteriocin-mediated inhibition of some common pathogens by wild and mutant Lactobacillus species and in vitro amplification of bacteriocin encoding genes10.5599/admet.10531848-7718https://doaj.org/article/811c6f0cbe2843cb93b52ba3c1b732db2021-11-01T00:00:00Zhttps://pub.iapchem.org/ojs/index.php/admet/article/view/1053https://doaj.org/toc/1848-7718 Lactobacilli are the most common probiotics used in food and other industries because of their capability of producing bacteriocins. Bacteriocins are compounds that are used to kill pathogenic microorganisms. As most bacteria have become resistant to synthetic antibacterial tools, the importance of using probiotics as antibacterial agents has increased. This work was done to check the bacteriocin effect on some common pathogens and the influence of mutation on the bacteriocin activity of Lactobacilli was also investigated. Four strains were isolated, identified from meat and pickles samples via culturing methods, staining, biochemical tests, and ribotyping. Preliminary tests, including Gram staining and catalase test, were done for the confirmation of Lactobacillus species. All strains were gram-positive and catalase-negative. Antibacterial activity was checked against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus thuringiensis, Escherichia coli, and Salmonella enteritis via agar well diffusion method. The mutations were done using ethidium bromide and the influence of wild and mutants were also checked. Interestingly, mutants developed more virulence than wild ones. It was also observed that they all were sensitive to pepsin. Protein estimation was done via Bradford method. Ribotyping of GCU-W-PS1 revealed 99 % homology with Lactobacillus plantarum and GCU-W-MS1 to Lactobacillus curvatus (99 % homology). Curvacin A, sakacin P, and plantaricin A genes were also amplified using specific primers. Gene sequence showed the presence of curvacin A gene in GCU-W-MS1. It was concluded that lactic acid bacteria could be used as antibacterial tools against common pathogens. Arooj AhsanBushra MazharMuhammad Kamran KhanMadiha MustafaMuhammad HammadNazish Mazhar AliInternational Association of Physical Chemists (IAPC)articleTherapeutics. PharmacologyRM1-950ENADMET and DMPK (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Therapeutics. Pharmacology RM1-950
spellingShingle	Therapeutics. Pharmacology RM1-950 Arooj Ahsan Bushra Mazhar Muhammad Kamran Khan Madiha Mustafa Muhammad Hammad Nazish Mazhar Ali Bacteriocin-mediated inhibition of some common pathogens by wild and mutant Lactobacillus species and in vitro amplification of bacteriocin encoding genes
description	Lactobacilli are the most common probiotics used in food and other industries because of their capability of producing bacteriocins. Bacteriocins are compounds that are used to kill pathogenic microorganisms. As most bacteria have become resistant to synthetic antibacterial tools, the importance of using probiotics as antibacterial agents has increased. This work was done to check the bacteriocin effect on some common pathogens and the influence of mutation on the bacteriocin activity of Lactobacilli was also investigated. Four strains were isolated, identified from meat and pickles samples via culturing methods, staining, biochemical tests, and ribotyping. Preliminary tests, including Gram staining and catalase test, were done for the confirmation of Lactobacillus species. All strains were gram-positive and catalase-negative. Antibacterial activity was checked against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus thuringiensis, Escherichia coli, and Salmonella enteritis via agar well diffusion method. The mutations were done using ethidium bromide and the influence of wild and mutants were also checked. Interestingly, mutants developed more virulence than wild ones. It was also observed that they all were sensitive to pepsin. Protein estimation was done via Bradford method. Ribotyping of GCU-W-PS1 revealed 99 % homology with Lactobacillus plantarum and GCU-W-MS1 to Lactobacillus curvatus (99 % homology). Curvacin A, sakacin P, and plantaricin A genes were also amplified using specific primers. Gene sequence showed the presence of curvacin A gene in GCU-W-MS1. It was concluded that lactic acid bacteria could be used as antibacterial tools against common pathogens.
format	article
author	Arooj Ahsan Bushra Mazhar Muhammad Kamran Khan Madiha Mustafa Muhammad Hammad Nazish Mazhar Ali
author_facet	Arooj Ahsan Bushra Mazhar Muhammad Kamran Khan Madiha Mustafa Muhammad Hammad Nazish Mazhar Ali
author_sort	Arooj Ahsan
title	Bacteriocin-mediated inhibition of some common pathogens by wild and mutant Lactobacillus species and in vitro amplification of bacteriocin encoding genes
title_short	Bacteriocin-mediated inhibition of some common pathogens by wild and mutant Lactobacillus species and in vitro amplification of bacteriocin encoding genes
title_full	Bacteriocin-mediated inhibition of some common pathogens by wild and mutant Lactobacillus species and in vitro amplification of bacteriocin encoding genes
title_fullStr	Bacteriocin-mediated inhibition of some common pathogens by wild and mutant Lactobacillus species and in vitro amplification of bacteriocin encoding genes
title_full_unstemmed	Bacteriocin-mediated inhibition of some common pathogens by wild and mutant Lactobacillus species and in vitro amplification of bacteriocin encoding genes
title_sort	bacteriocin-mediated inhibition of some common pathogens by wild and mutant lactobacillus species and in vitro amplification of bacteriocin encoding genes
publisher	International Association of Physical Chemists (IAPC)
publishDate	2021
url	https://doaj.org/article/811c6f0cbe2843cb93b52ba3c1b732db
work_keys_str_mv	AT aroojahsan bacteriocinmediatedinhibitionofsomecommonpathogensbywildandmutantlactobacillusspeciesandinvitroamplificationofbacteriocinencodinggenes AT bushramazhar bacteriocinmediatedinhibitionofsomecommonpathogensbywildandmutantlactobacillusspeciesandinvitroamplificationofbacteriocinencodinggenes AT muhammadkamrankhan bacteriocinmediatedinhibitionofsomecommonpathogensbywildandmutantlactobacillusspeciesandinvitroamplificationofbacteriocinencodinggenes AT madihamustafa bacteriocinmediatedinhibitionofsomecommonpathogensbywildandmutantlactobacillusspeciesandinvitroamplificationofbacteriocinencodinggenes AT muhammadhammad bacteriocinmediatedinhibitionofsomecommonpathogensbywildandmutantlactobacillusspeciesandinvitroamplificationofbacteriocinencodinggenes AT nazishmazharali bacteriocinmediatedinhibitionofsomecommonpathogensbywildandmutantlactobacillusspeciesandinvitroamplificationofbacteriocinencodinggenes
_version_	1718428444043247616

Bacteriocin-mediated inhibition of some common pathogens by wild and mutant Lactobacillus species and in vitro amplification of bacteriocin encoding genes

Ejemplares similares