Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug-delivery system

Farahnaz Barahuie,1,2 Dena Dorniani,1,3,* Bullo Saifullah,1,* Sivapragasam Gothai,4 Mohd Zobir Hussein,1 Ashok Kumar Pandurangan,5 Palanisamy Arulselvan,4 Mohd Esa Norhaizan6 1Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang,...

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Autores principales:	Barahuie F, Dorniani D, Saifullah B, Gothai S, Hussein MZ, Pandurangan AK, Arulselvan P, Norhaizan ME
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Nanocomposite Drug delivery Chitosan Phytic acid HT-29 cell line Controlled release Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8125da4c8e2d43f0931eeef981bc0e33
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spelling	oai:doaj.org-article:8125da4c8e2d43f0931eeef981bc0e332021-12-02T07:13:44ZSustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug-delivery system1178-2013https://doaj.org/article/8125da4c8e2d43f0931eeef981bc0e332017-03-01T00:00:00Zhttps://www.dovepress.com/sustained-release-of-anticancer-agent-phytic-acid-from-its-chitosan-co-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Farahnaz Barahuie,1,2 Dena Dorniani,1,3,* Bullo Saifullah,1,* Sivapragasam Gothai,4 Mohd Zobir Hussein,1 Ashok Kumar Pandurangan,5 Palanisamy Arulselvan,4 Mohd Esa Norhaizan6 1Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 2Zabol University of Medical Sciences, Zabol, Iran; 3Department of Chemistry, University of Sheffield, Sheffield, UK; 4Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 5Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; 6Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia *These authors contributed equally to this work Abstract: Chitosan (CS) iron oxide magnetic nanoparticles (MNPs) were coated with phytic acid (PTA) to form phytic acid-chitosan-iron oxide nanocomposite (PTA-CS-MNP). The obtained nanocomposite and nanocarrier were characterized by powder X-ray diffraction, Fourier transform infrared spectroscopy, vibrating sample magnetometry, transmission electron microscopy, and thermogravimetric and differential thermogravimetric analyses. Fourier transform infrared spectra and thermal analysis of MNPs and PTA-CS-MNP nanocomposite confirmed the binding of CS on the surface of MNPs and the loading of PTA in the PTA-CS-MNP nanocomposite. The coating process enhanced the thermal stability of the anticancer nanocomposite obtained. X-ray diffraction results showed that the MNPs and PTA-CS-MNP nanocomposite are pure magnetite. Drug loading was estimated using ultraviolet-visible spectroscopy and showing a 12.9% in the designed nanocomposite. Magnetization curves demonstrated that the synthesized MNPs and nanocomposite were superparamagnetic with saturation magnetizations of 53.25 emu/g and 42.15 emu/g, respectively. The release study showed that around 86% and 93% of PTA from PTA-CS-MNP nanocomposite could be released within 127 and 56 hours by a phosphate buffer solution at pH 7.4 and 4.8, respectively, in a sustained manner and governed by pseudo-second order kinetic model. The cytotoxicity of the compounds on HT-29 colon cancer cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The HT-29 cell line was more sensitive against PTA-CS-MNP nanocomposite than PTA alone. No cytotoxic effect was observed on normal cells (3T3 fibroblast cells). This result indicates that PTA-CS-MNP nanocomposite can inhibit the proliferation of colon cancer cells without causing any harm to normal cell. Keywords: nanocomposite, drug delivery, chitosan, phytic acid, HT-29 cell line, controlled releaseBarahuie FDorniani DSaifullah BGothai SHussein MZPandurangan AKArulselvan PNorhaizan MEDove Medical PressarticleNanocompositeDrug deliveryChitosanPhytic acidHT-29 cell lineControlled releaseMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2361-2372 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanocomposite Drug delivery Chitosan Phytic acid HT-29 cell line Controlled release Medicine (General) R5-920
spellingShingle	Nanocomposite Drug delivery Chitosan Phytic acid HT-29 cell line Controlled release Medicine (General) R5-920 Barahuie F Dorniani D Saifullah B Gothai S Hussein MZ Pandurangan AK Arulselvan P Norhaizan ME Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug-delivery system
description	Farahnaz Barahuie,1,2 Dena Dorniani,1,3,* Bullo Saifullah,1,* Sivapragasam Gothai,4 Mohd Zobir Hussein,1 Ashok Kumar Pandurangan,5 Palanisamy Arulselvan,4 Mohd Esa Norhaizan6 1Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 2Zabol University of Medical Sciences, Zabol, Iran; 3Department of Chemistry, University of Sheffield, Sheffield, UK; 4Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 5Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; 6Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia *These authors contributed equally to this work Abstract: Chitosan (CS) iron oxide magnetic nanoparticles (MNPs) were coated with phytic acid (PTA) to form phytic acid-chitosan-iron oxide nanocomposite (PTA-CS-MNP). The obtained nanocomposite and nanocarrier were characterized by powder X-ray diffraction, Fourier transform infrared spectroscopy, vibrating sample magnetometry, transmission electron microscopy, and thermogravimetric and differential thermogravimetric analyses. Fourier transform infrared spectra and thermal analysis of MNPs and PTA-CS-MNP nanocomposite confirmed the binding of CS on the surface of MNPs and the loading of PTA in the PTA-CS-MNP nanocomposite. The coating process enhanced the thermal stability of the anticancer nanocomposite obtained. X-ray diffraction results showed that the MNPs and PTA-CS-MNP nanocomposite are pure magnetite. Drug loading was estimated using ultraviolet-visible spectroscopy and showing a 12.9% in the designed nanocomposite. Magnetization curves demonstrated that the synthesized MNPs and nanocomposite were superparamagnetic with saturation magnetizations of 53.25 emu/g and 42.15 emu/g, respectively. The release study showed that around 86% and 93% of PTA from PTA-CS-MNP nanocomposite could be released within 127 and 56 hours by a phosphate buffer solution at pH 7.4 and 4.8, respectively, in a sustained manner and governed by pseudo-second order kinetic model. The cytotoxicity of the compounds on HT-29 colon cancer cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The HT-29 cell line was more sensitive against PTA-CS-MNP nanocomposite than PTA alone. No cytotoxic effect was observed on normal cells (3T3 fibroblast cells). This result indicates that PTA-CS-MNP nanocomposite can inhibit the proliferation of colon cancer cells without causing any harm to normal cell. Keywords: nanocomposite, drug delivery, chitosan, phytic acid, HT-29 cell line, controlled release
format	article
author	Barahuie F Dorniani D Saifullah B Gothai S Hussein MZ Pandurangan AK Arulselvan P Norhaizan ME
author_facet	Barahuie F Dorniani D Saifullah B Gothai S Hussein MZ Pandurangan AK Arulselvan P Norhaizan ME
author_sort	Barahuie F
title	Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug-delivery system
title_short	Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug-delivery system
title_full	Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug-delivery system
title_fullStr	Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug-delivery system
title_full_unstemmed	Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug-delivery system
title_sort	sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug-delivery system
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/8125da4c8e2d43f0931eeef981bc0e33
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_version_	1718399523297951744

Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug-delivery system

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