Establishment of the model system between phytochemicals and gene expression profiles in Macrosclereid cells of Medicago truncatula

Abstract Macrosclereid cells, which are a layer in the seed coat of Medicago truncatula, accumulate large amounts of phytochemicals during their development. But little is known about the complex and dynamic changes during macrosclereid cell development. To characterize the phytochemicals and the re...

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Autores principales:	Fuyou Fu, Wentao Zhang, Yuan-Yuan Li, Hong Li Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/81277bf9511d46a8b548d2ef564f3943
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spelling	oai:doaj.org-article:81277bf9511d46a8b548d2ef564f39432021-12-02T12:30:12ZEstablishment of the model system between phytochemicals and gene expression profiles in Macrosclereid cells of Medicago truncatula10.1038/s41598-017-02827-52045-2322https://doaj.org/article/81277bf9511d46a8b548d2ef564f39432017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02827-5https://doaj.org/toc/2045-2322Abstract Macrosclereid cells, which are a layer in the seed coat of Medicago truncatula, accumulate large amounts of phytochemicals during their development. But little is known about the complex and dynamic changes during macrosclereid cell development. To characterize the phytochemicals and the related gene expression during the development of M. truncatula macrosclereid cells, a high performance liquid chromatography-mass spectrometry (HPLC-MS) assay and microarray study were conducted on transcriptome changes from macrosclereid cell during seed development. A total of 16 flavonoids by HPLC-MS and 4861 genes exhibited significant differences at transcript levels by microarray analysis were identified for macrosclerid cells at six different time points during seed development. 815 abiotic and biotic stress genes, 223 transcriptional factors (TFs), and 155 annotated transporter proteins exhibited differential expression during the development of macrosclereid cells. A total of 102 genes were identified as involved in flavonoid biosynthesis, phenypropanoid biosynthesis, and flavone and flavonol biosynthesis. We performed a weighted gene co-regulatory network (WGCNA) to analyze the gene-flavonoid association and rebuilt the gene regulatory network during macrosclereid cell development. Our studies revealed that macrosclereid cells are, beside as the first barrier of defense against diseases, an excellent model system to investigate the regulatory network that governs flavonoid biosynthesis.Fuyou FuWentao ZhangYuan-Yuan LiHong Li WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fuyou Fu Wentao Zhang Yuan-Yuan Li Hong Li Wang Establishment of the model system between phytochemicals and gene expression profiles in Macrosclereid cells of Medicago truncatula
description	Abstract Macrosclereid cells, which are a layer in the seed coat of Medicago truncatula, accumulate large amounts of phytochemicals during their development. But little is known about the complex and dynamic changes during macrosclereid cell development. To characterize the phytochemicals and the related gene expression during the development of M. truncatula macrosclereid cells, a high performance liquid chromatography-mass spectrometry (HPLC-MS) assay and microarray study were conducted on transcriptome changes from macrosclereid cell during seed development. A total of 16 flavonoids by HPLC-MS and 4861 genes exhibited significant differences at transcript levels by microarray analysis were identified for macrosclerid cells at six different time points during seed development. 815 abiotic and biotic stress genes, 223 transcriptional factors (TFs), and 155 annotated transporter proteins exhibited differential expression during the development of macrosclereid cells. A total of 102 genes were identified as involved in flavonoid biosynthesis, phenypropanoid biosynthesis, and flavone and flavonol biosynthesis. We performed a weighted gene co-regulatory network (WGCNA) to analyze the gene-flavonoid association and rebuilt the gene regulatory network during macrosclereid cell development. Our studies revealed that macrosclereid cells are, beside as the first barrier of defense against diseases, an excellent model system to investigate the regulatory network that governs flavonoid biosynthesis.
format	article
author	Fuyou Fu Wentao Zhang Yuan-Yuan Li Hong Li Wang
author_facet	Fuyou Fu Wentao Zhang Yuan-Yuan Li Hong Li Wang
author_sort	Fuyou Fu
title	Establishment of the model system between phytochemicals and gene expression profiles in Macrosclereid cells of Medicago truncatula
title_short	Establishment of the model system between phytochemicals and gene expression profiles in Macrosclereid cells of Medicago truncatula
title_full	Establishment of the model system between phytochemicals and gene expression profiles in Macrosclereid cells of Medicago truncatula
title_fullStr	Establishment of the model system between phytochemicals and gene expression profiles in Macrosclereid cells of Medicago truncatula
title_full_unstemmed	Establishment of the model system between phytochemicals and gene expression profiles in Macrosclereid cells of Medicago truncatula
title_sort	establishment of the model system between phytochemicals and gene expression profiles in macrosclereid cells of medicago truncatula
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/81277bf9511d46a8b548d2ef564f3943
work_keys_str_mv	AT fuyoufu establishmentofthemodelsystembetweenphytochemicalsandgeneexpressionprofilesinmacrosclereidcellsofmedicagotruncatula AT wentaozhang establishmentofthemodelsystembetweenphytochemicalsandgeneexpressionprofilesinmacrosclereidcellsofmedicagotruncatula AT yuanyuanli establishmentofthemodelsystembetweenphytochemicalsandgeneexpressionprofilesinmacrosclereidcellsofmedicagotruncatula AT hongliwang establishmentofthemodelsystembetweenphytochemicalsandgeneexpressionprofilesinmacrosclereidcellsofmedicagotruncatula
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Establishment of the model system between phytochemicals and gene expression profiles in Macrosclereid cells of Medicago truncatula

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