A preliminary analysis of volatile metabolites of human induced pluripotent stem cells along the in vitro differentiation

Abstract Cellular metabolism of stem cell biology is still an unexplored field. However, considering the amount of information carried by metabolomes, this is a promising field for a fast identification of stem cells itself and during the differentiation process. One of the goals of such application...

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Autores principales:	Rosamaria Capuano, Paola Spitalieri, Rosa Valentina Talarico, Ana Carolina Domakoski, Alexandro Catini, Roberto Paolesse, Eugenio Martinelli, Giuseppe Novelli, Federica Sangiuolo, Corrado Di Natale
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/812f86c2db0f491c84d6bc6ed303bce3
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spelling	oai:doaj.org-article:812f86c2db0f491c84d6bc6ed303bce32021-12-02T16:06:27ZA preliminary analysis of volatile metabolites of human induced pluripotent stem cells along the in vitro differentiation10.1038/s41598-017-01790-52045-2322https://doaj.org/article/812f86c2db0f491c84d6bc6ed303bce32017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01790-5https://doaj.org/toc/2045-2322Abstract Cellular metabolism of stem cell biology is still an unexplored field. However, considering the amount of information carried by metabolomes, this is a promising field for a fast identification of stem cells itself and during the differentiation process. One of the goals of such application is the identification of residual pluripotent cells before cell transplantation to avoid the occurrence of teratomas. In this paper, we investigated in vitro the volatile compounds (VOCs) released during human induced pluripotent stem cells (hiPSCs) reprogramming. In particular, we studied hiPSCs differentiation to floating and adherent embryoid bodies until early neural progenitor cells. A preliminary Gas Chromatographic/Mass Spectrometer (GC/MS) analysis, based on a single extraction method and chromatographic separation, indicated 17 volatile compounds whose relative abundance is altered in each step of the differentiation process. The pattern of VOCs shown by hiPSCs is well distinct and makes these cells sharply separated from the other steps of differentiations. Similar behaviour has also been observed with an array of metalloporphyrins based gas sensors. The use of electronic sensors to control the process of differentiation of pluripotent stem cells might suggest a novel perspective for a fast and on-line control of differentiation processes.Rosamaria CapuanoPaola SpitalieriRosa Valentina TalaricoAna Carolina DomakoskiAlexandro CatiniRoberto PaolesseEugenio MartinelliGiuseppe NovelliFederica SangiuoloCorrado Di NataleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rosamaria Capuano Paola Spitalieri Rosa Valentina Talarico Ana Carolina Domakoski Alexandro Catini Roberto Paolesse Eugenio Martinelli Giuseppe Novelli Federica Sangiuolo Corrado Di Natale A preliminary analysis of volatile metabolites of human induced pluripotent stem cells along the in vitro differentiation
description	Abstract Cellular metabolism of stem cell biology is still an unexplored field. However, considering the amount of information carried by metabolomes, this is a promising field for a fast identification of stem cells itself and during the differentiation process. One of the goals of such application is the identification of residual pluripotent cells before cell transplantation to avoid the occurrence of teratomas. In this paper, we investigated in vitro the volatile compounds (VOCs) released during human induced pluripotent stem cells (hiPSCs) reprogramming. In particular, we studied hiPSCs differentiation to floating and adherent embryoid bodies until early neural progenitor cells. A preliminary Gas Chromatographic/Mass Spectrometer (GC/MS) analysis, based on a single extraction method and chromatographic separation, indicated 17 volatile compounds whose relative abundance is altered in each step of the differentiation process. The pattern of VOCs shown by hiPSCs is well distinct and makes these cells sharply separated from the other steps of differentiations. Similar behaviour has also been observed with an array of metalloporphyrins based gas sensors. The use of electronic sensors to control the process of differentiation of pluripotent stem cells might suggest a novel perspective for a fast and on-line control of differentiation processes.
format	article
author	Rosamaria Capuano Paola Spitalieri Rosa Valentina Talarico Ana Carolina Domakoski Alexandro Catini Roberto Paolesse Eugenio Martinelli Giuseppe Novelli Federica Sangiuolo Corrado Di Natale
author_facet	Rosamaria Capuano Paola Spitalieri Rosa Valentina Talarico Ana Carolina Domakoski Alexandro Catini Roberto Paolesse Eugenio Martinelli Giuseppe Novelli Federica Sangiuolo Corrado Di Natale
author_sort	Rosamaria Capuano
title	A preliminary analysis of volatile metabolites of human induced pluripotent stem cells along the in vitro differentiation
title_short	A preliminary analysis of volatile metabolites of human induced pluripotent stem cells along the in vitro differentiation
title_full	A preliminary analysis of volatile metabolites of human induced pluripotent stem cells along the in vitro differentiation
title_fullStr	A preliminary analysis of volatile metabolites of human induced pluripotent stem cells along the in vitro differentiation
title_full_unstemmed	A preliminary analysis of volatile metabolites of human induced pluripotent stem cells along the in vitro differentiation
title_sort	preliminary analysis of volatile metabolites of human induced pluripotent stem cells along the in vitro differentiation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/812f86c2db0f491c84d6bc6ed303bce3
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A preliminary analysis of volatile metabolites of human induced pluripotent stem cells along the in vitro differentiation

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