The human respiratory tract microbial community structures in healthy and cystic fibrosis infants

Abstract The metagenome development of the human respiratory tract was investigated by shotgun metagenome metagenomic sequencing of cough swabs from healthy children and children with cystic fibrosis (CF) between 3 weeks and 6 years of age. A healthy microbial community signature was associated with...

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Autores principales:	Marie-Madlen Pust, Lutz Wiehlmann, Colin Davenport, Isa Rudolf, Anna-Maria Dittrich, Burkhard Tümmler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/812ff24a9f9e4967b64b187247eaaf4d
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spelling	oai:doaj.org-article:812ff24a9f9e4967b64b187247eaaf4d2021-12-02T12:04:06ZThe human respiratory tract microbial community structures in healthy and cystic fibrosis infants10.1038/s41522-020-00171-72055-5008https://doaj.org/article/812ff24a9f9e4967b64b187247eaaf4d2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41522-020-00171-7https://doaj.org/toc/2055-5008Abstract The metagenome development of the human respiratory tract was investigated by shotgun metagenome metagenomic sequencing of cough swabs from healthy children and children with cystic fibrosis (CF) between 3 weeks and 6 years of age. A healthy microbial community signature was associated with increased absolute abundances in terms of bacterial–human cell ratios of core and rare species across all age groups, with a higher diversity of rare species and a tightly interconnected species co-occurrence network, in which individual members were found in close proximity to each other and negative correlations were absent. Even without typical CF pathogens, the CF infant co-occurrence network was found to be less stable and prone to fragmentation due to fewer connections between species, a higher number of bridging species and the presence of negative species correlations. Detection of low-abundant DNA of the CF hallmark pathogen Pseudomonas aeruginosa was neither disease- nor age-associated in our cohort. Healthy and CF children come into contact with P. aeruginosa on a regular basis and from early on.Marie-Madlen PustLutz WiehlmannColin DavenportIsa RudolfAnna-Maria DittrichBurkhard TümmlerNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 6, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Marie-Madlen Pust Lutz Wiehlmann Colin Davenport Isa Rudolf Anna-Maria Dittrich Burkhard Tümmler The human respiratory tract microbial community structures in healthy and cystic fibrosis infants
description	Abstract The metagenome development of the human respiratory tract was investigated by shotgun metagenome metagenomic sequencing of cough swabs from healthy children and children with cystic fibrosis (CF) between 3 weeks and 6 years of age. A healthy microbial community signature was associated with increased absolute abundances in terms of bacterial–human cell ratios of core and rare species across all age groups, with a higher diversity of rare species and a tightly interconnected species co-occurrence network, in which individual members were found in close proximity to each other and negative correlations were absent. Even without typical CF pathogens, the CF infant co-occurrence network was found to be less stable and prone to fragmentation due to fewer connections between species, a higher number of bridging species and the presence of negative species correlations. Detection of low-abundant DNA of the CF hallmark pathogen Pseudomonas aeruginosa was neither disease- nor age-associated in our cohort. Healthy and CF children come into contact with P. aeruginosa on a regular basis and from early on.
format	article
author	Marie-Madlen Pust Lutz Wiehlmann Colin Davenport Isa Rudolf Anna-Maria Dittrich Burkhard Tümmler
author_facet	Marie-Madlen Pust Lutz Wiehlmann Colin Davenport Isa Rudolf Anna-Maria Dittrich Burkhard Tümmler
author_sort	Marie-Madlen Pust
title	The human respiratory tract microbial community structures in healthy and cystic fibrosis infants
title_short	The human respiratory tract microbial community structures in healthy and cystic fibrosis infants
title_full	The human respiratory tract microbial community structures in healthy and cystic fibrosis infants
title_fullStr	The human respiratory tract microbial community structures in healthy and cystic fibrosis infants
title_full_unstemmed	The human respiratory tract microbial community structures in healthy and cystic fibrosis infants
title_sort	human respiratory tract microbial community structures in healthy and cystic fibrosis infants
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/812ff24a9f9e4967b64b187247eaaf4d
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The human respiratory tract microbial community structures in healthy and cystic fibrosis infants

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