Segmented flow generator for serial crystallography at the European X-ray free electron laser

Due to the pulsed nature of X-ray free electron laser (XFEL) instruments the majority of protein crystals, which are injected using continuous jet injection techniques are wasted. Here, the authors present a microfluidic device to deliver aqueous protein crystal laden droplets segmented with an immi...

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Autores principales:	Austin Echelmeier, Jorvani Cruz Villarreal, Marc Messerschmidt, Daihyun Kim, Jesse D. Coe, Darren Thifault, Sabine Botha, Ana Egatz-Gomez, Sahir Gandhi, Gerrit Brehm, Chelsie E. Conrad, Debra T. Hansen, Caleb Madsen, Saša Bajt, J. Domingo Meza-Aguilar, Dominik Oberthür, Max O. Wiedorn, Holger Fleckenstein, Derek Mendez, Juraj Knoška, Jose M. Martin-Garcia, Hao Hu, Stella Lisova, Aschkan Allahgholi, Yaroslav Gevorkov, Kartik Ayyer, Steve Aplin, Helen Mary Ginn, Heinz Graafsma, Andrew J. Morgan, Dominic Greiffenberg, Alexander Klujev, Torsten Laurus, Jennifer Poehlsen, Ulrich Trunk, Davide Mezza, Bernd Schmidt, Manuela Kuhn, Raimund Fromme, Jolanta Sztuk-Dambietz, Natascha Raab, Steffen Hauf, Alessandro Silenzi, Thomas Michelat, Chen Xu, Cyril Danilevski, Andrea Parenti, Leonce Mekinda, Britta Weinhausen, Grant Mills, Patrik Vagovic, Yoonhee Kim, Henry Kirkwood, Richard Bean, Johan Bielecki, Stephan Stern, Klaus Giewekemeyer, Adam R. Round, Joachim Schulz, Katerina Dörner, Thomas D. Grant, Valerio Mariani, Anton Barty, Adrian P. Mancuso, Uwe Weierstall, John C. H. Spence, Henry N. Chapman, Nadia Zatsepin, Petra Fromme, Richard A. Kirian, Alexandra Ros
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/813507b66a6b4fc7a68f618e55926b0f
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spelling	oai:doaj.org-article:813507b66a6b4fc7a68f618e55926b0f2021-12-02T19:13:48ZSegmented flow generator for serial crystallography at the European X-ray free electron laser10.1038/s41467-020-18156-72041-1723https://doaj.org/article/813507b66a6b4fc7a68f618e55926b0f2020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18156-7https://doaj.org/toc/2041-1723Due to the pulsed nature of X-ray free electron laser (XFEL) instruments the majority of protein crystals, which are injected using continuous jet injection techniques are wasted. Here, the authors present a microfluidic device to deliver aqueous protein crystal laden droplets segmented with an immiscible oil and demonstrate that with this device an approx. 60% reduction in sample waste was achieved for data collection of 3-deoxy-D-manno-octulosonate 8-phosphate synthase crystals at the EuXFEL.Austin EchelmeierJorvani Cruz VillarrealMarc MesserschmidtDaihyun KimJesse D. CoeDarren ThifaultSabine BothaAna Egatz-GomezSahir GandhiGerrit BrehmChelsie E. ConradDebra T. HansenCaleb MadsenSaša BajtJ. Domingo Meza-AguilarDominik OberthürMax O. WiedornHolger FleckensteinDerek MendezJuraj KnoškaJose M. Martin-GarciaHao HuStella LisovaAschkan AllahgholiYaroslav GevorkovKartik AyyerSteve AplinHelen Mary GinnHeinz GraafsmaAndrew J. MorganDominic GreiffenbergAlexander KlujevTorsten LaurusJennifer PoehlsenUlrich TrunkDavide MezzaBernd SchmidtManuela KuhnRaimund FrommeJolanta Sztuk-DambietzNatascha RaabSteffen HaufAlessandro SilenziThomas MichelatChen XuCyril DanilevskiAndrea ParentiLeonce MekindaBritta WeinhausenGrant MillsPatrik VagovicYoonhee KimHenry KirkwoodRichard BeanJohan BieleckiStephan SternKlaus GiewekemeyerAdam R. RoundJoachim SchulzKaterina DörnerThomas D. GrantValerio MarianiAnton BartyAdrian P. MancusoUwe WeierstallJohn C. H. SpenceHenry N. ChapmanNadia ZatsepinPetra FrommeRichard A. KirianAlexandra RosNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Austin Echelmeier Jorvani Cruz Villarreal Marc Messerschmidt Daihyun Kim Jesse D. Coe Darren Thifault Sabine Botha Ana Egatz-Gomez Sahir Gandhi Gerrit Brehm Chelsie E. Conrad Debra T. Hansen Caleb Madsen Saša Bajt J. Domingo Meza-Aguilar Dominik Oberthür Max O. Wiedorn Holger Fleckenstein Derek Mendez Juraj Knoška Jose M. Martin-Garcia Hao Hu Stella Lisova Aschkan Allahgholi Yaroslav Gevorkov Kartik Ayyer Steve Aplin Helen Mary Ginn Heinz Graafsma Andrew J. Morgan Dominic Greiffenberg Alexander Klujev Torsten Laurus Jennifer Poehlsen Ulrich Trunk Davide Mezza Bernd Schmidt Manuela Kuhn Raimund Fromme Jolanta Sztuk-Dambietz Natascha Raab Steffen Hauf Alessandro Silenzi Thomas Michelat Chen Xu Cyril Danilevski Andrea Parenti Leonce Mekinda Britta Weinhausen Grant Mills Patrik Vagovic Yoonhee Kim Henry Kirkwood Richard Bean Johan Bielecki Stephan Stern Klaus Giewekemeyer Adam R. Round Joachim Schulz Katerina Dörner Thomas D. Grant Valerio Mariani Anton Barty Adrian P. Mancuso Uwe Weierstall John C. H. Spence Henry N. Chapman Nadia Zatsepin Petra Fromme Richard A. Kirian Alexandra Ros Segmented flow generator for serial crystallography at the European X-ray free electron laser
description	Due to the pulsed nature of X-ray free electron laser (XFEL) instruments the majority of protein crystals, which are injected using continuous jet injection techniques are wasted. Here, the authors present a microfluidic device to deliver aqueous protein crystal laden droplets segmented with an immiscible oil and demonstrate that with this device an approx. 60% reduction in sample waste was achieved for data collection of 3-deoxy-D-manno-octulosonate 8-phosphate synthase crystals at the EuXFEL.
format	article
author	Austin Echelmeier Jorvani Cruz Villarreal Marc Messerschmidt Daihyun Kim Jesse D. Coe Darren Thifault Sabine Botha Ana Egatz-Gomez Sahir Gandhi Gerrit Brehm Chelsie E. Conrad Debra T. Hansen Caleb Madsen Saša Bajt J. Domingo Meza-Aguilar Dominik Oberthür Max O. Wiedorn Holger Fleckenstein Derek Mendez Juraj Knoška Jose M. Martin-Garcia Hao Hu Stella Lisova Aschkan Allahgholi Yaroslav Gevorkov Kartik Ayyer Steve Aplin Helen Mary Ginn Heinz Graafsma Andrew J. Morgan Dominic Greiffenberg Alexander Klujev Torsten Laurus Jennifer Poehlsen Ulrich Trunk Davide Mezza Bernd Schmidt Manuela Kuhn Raimund Fromme Jolanta Sztuk-Dambietz Natascha Raab Steffen Hauf Alessandro Silenzi Thomas Michelat Chen Xu Cyril Danilevski Andrea Parenti Leonce Mekinda Britta Weinhausen Grant Mills Patrik Vagovic Yoonhee Kim Henry Kirkwood Richard Bean Johan Bielecki Stephan Stern Klaus Giewekemeyer Adam R. Round Joachim Schulz Katerina Dörner Thomas D. Grant Valerio Mariani Anton Barty Adrian P. Mancuso Uwe Weierstall John C. H. Spence Henry N. Chapman Nadia Zatsepin Petra Fromme Richard A. Kirian Alexandra Ros
author_facet	Austin Echelmeier Jorvani Cruz Villarreal Marc Messerschmidt Daihyun Kim Jesse D. Coe Darren Thifault Sabine Botha Ana Egatz-Gomez Sahir Gandhi Gerrit Brehm Chelsie E. Conrad Debra T. Hansen Caleb Madsen Saša Bajt J. Domingo Meza-Aguilar Dominik Oberthür Max O. Wiedorn Holger Fleckenstein Derek Mendez Juraj Knoška Jose M. Martin-Garcia Hao Hu Stella Lisova Aschkan Allahgholi Yaroslav Gevorkov Kartik Ayyer Steve Aplin Helen Mary Ginn Heinz Graafsma Andrew J. Morgan Dominic Greiffenberg Alexander Klujev Torsten Laurus Jennifer Poehlsen Ulrich Trunk Davide Mezza Bernd Schmidt Manuela Kuhn Raimund Fromme Jolanta Sztuk-Dambietz Natascha Raab Steffen Hauf Alessandro Silenzi Thomas Michelat Chen Xu Cyril Danilevski Andrea Parenti Leonce Mekinda Britta Weinhausen Grant Mills Patrik Vagovic Yoonhee Kim Henry Kirkwood Richard Bean Johan Bielecki Stephan Stern Klaus Giewekemeyer Adam R. Round Joachim Schulz Katerina Dörner Thomas D. Grant Valerio Mariani Anton Barty Adrian P. Mancuso Uwe Weierstall John C. H. Spence Henry N. Chapman Nadia Zatsepin Petra Fromme Richard A. Kirian Alexandra Ros
author_sort	Austin Echelmeier
title	Segmented flow generator for serial crystallography at the European X-ray free electron laser
title_short	Segmented flow generator for serial crystallography at the European X-ray free electron laser
title_full	Segmented flow generator for serial crystallography at the European X-ray free electron laser
title_fullStr	Segmented flow generator for serial crystallography at the European X-ray free electron laser
title_full_unstemmed	Segmented flow generator for serial crystallography at the European X-ray free electron laser
title_sort	segmented flow generator for serial crystallography at the european x-ray free electron laser
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/813507b66a6b4fc7a68f618e55926b0f
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Segmented flow generator for serial crystallography at the European X-ray free electron laser

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