Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production

Abstract Bombyx mori (B. mori) is important due to its major role in the silk production. Though DNA binding ligands often influence gene expression, no attempt has been made to exploit their use in sericulture. The telomeric heterochromatin of B. mori is enriched with 5′-TTAGG-3′ sequences. These s...

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Autores principales:	Asfa Ali, Venugopal Reddy Bovilla, Danti Kumari Mysarla, Prasanthi Siripurapu, Rashmi U. Pathak, Bhakti Basu, Anitha Mamillapalli, Santanu Bhattacharya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8135f41fe5114d22a31e8ba15fe98dd5
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spelling	oai:doaj.org-article:8135f41fe5114d22a31e8ba15fe98dd52021-12-02T12:30:13ZKnockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production10.1038/s41598-017-00653-32045-2322https://doaj.org/article/8135f41fe5114d22a31e8ba15fe98dd52017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00653-3https://doaj.org/toc/2045-2322Abstract Bombyx mori (B. mori) is important due to its major role in the silk production. Though DNA binding ligands often influence gene expression, no attempt has been made to exploit their use in sericulture. The telomeric heterochromatin of B. mori is enriched with 5′-TTAGG-3′ sequences. These sequences were also found to be present in several genes in the euchromatic regions. We examined three synthetic oligopyrrole carboxamides that target 5′-TTAGG-3′ sequences in controlling the gene expression in B. mori. The ligands did not show any defect or feeding difference in the larval stage, crucial for silk production. The ligands caused silencing of various isoforms of the broad-complex transcription factor and cuticle proteins which resulted in late pupal developmental defects. Furthermore, treatment with such drugs resulted in statistically enhanced cocoon weight, shell weight, and silk yield. This study shows for the first time use of oligopyrrole carboxamide drugs in controlling gene expression in B. mori and their long term use in enhancing silk production.Asfa AliVenugopal Reddy BovillaDanti Kumari MysarlaPrasanthi SiripurapuRashmi U. PathakBhakti BasuAnitha MamillapalliSantanu BhattacharyaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Asfa Ali Venugopal Reddy Bovilla Danti Kumari Mysarla Prasanthi Siripurapu Rashmi U. Pathak Bhakti Basu Anitha Mamillapalli Santanu Bhattacharya Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production
description	Abstract Bombyx mori (B. mori) is important due to its major role in the silk production. Though DNA binding ligands often influence gene expression, no attempt has been made to exploit their use in sericulture. The telomeric heterochromatin of B. mori is enriched with 5′-TTAGG-3′ sequences. These sequences were also found to be present in several genes in the euchromatic regions. We examined three synthetic oligopyrrole carboxamides that target 5′-TTAGG-3′ sequences in controlling the gene expression in B. mori. The ligands did not show any defect or feeding difference in the larval stage, crucial for silk production. The ligands caused silencing of various isoforms of the broad-complex transcription factor and cuticle proteins which resulted in late pupal developmental defects. Furthermore, treatment with such drugs resulted in statistically enhanced cocoon weight, shell weight, and silk yield. This study shows for the first time use of oligopyrrole carboxamide drugs in controlling gene expression in B. mori and their long term use in enhancing silk production.
format	article
author	Asfa Ali Venugopal Reddy Bovilla Danti Kumari Mysarla Prasanthi Siripurapu Rashmi U. Pathak Bhakti Basu Anitha Mamillapalli Santanu Bhattacharya
author_facet	Asfa Ali Venugopal Reddy Bovilla Danti Kumari Mysarla Prasanthi Siripurapu Rashmi U. Pathak Bhakti Basu Anitha Mamillapalli Santanu Bhattacharya
author_sort	Asfa Ali
title	Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production
title_short	Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production
title_full	Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production
title_fullStr	Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production
title_full_unstemmed	Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production
title_sort	knockdown of broad-complex gene expression of bombyx mori by oligopyrrole carboxamides enhances silk production
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8135f41fe5114d22a31e8ba15fe98dd5
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Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production

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