DNA barcoding of Crustacean larvae in the eastern areas of Segara Anakan Cilacap, Central Java Indonesia

Abstract. Kusbiyanto, Bhagawati D, Nuryanto A. 2020. DNA barcoding of crustacean larvae in Segara Anakan, Cilacap, Central Java, Indonesia using cytochrome c oxidase gene. Biodiversitas 21: 4878-4887. Species-level identification of crustacean larvae is challenging due to morphological constraints....

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Autores principales:	KUSBIYANTO KUSBIYANTO, DIAN BHAGAWATI, Agus Nuryanto
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	barcoding, crustacea, cytochrome c oxidase gene, larvae, species identification Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/813b16d115f44241897c44707bc5849d
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spelling	oai:doaj.org-article:813b16d115f44241897c44707bc5849d2021-11-22T00:51:08ZDNA barcoding of Crustacean larvae in the eastern areas of Segara Anakan Cilacap, Central Java Indonesia1412-033X2085-472210.13057/biodiv/d211054https://doaj.org/article/813b16d115f44241897c44707bc5849d2020-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6588https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Kusbiyanto, Bhagawati D, Nuryanto A. 2020. DNA barcoding of crustacean larvae in Segara Anakan, Cilacap, Central Java, Indonesia using cytochrome c oxidase gene. Biodiversitas 21: 4878-4887. Species-level identification of crustacean larvae is challenging due to morphological constraints. DNA barcoding offers a precise method to solve the problems. That method has never been applied to crustacean larvae from the eastern of Segara Anakan, Cilacap, Central Java, Indonesia. This study aims to identify crustacean larvae in the eastern of Segara Anakan using the cytochrome c oxidase subunit I (COI) gene as a barcode marker. Larvae morphotypes were identified under a binocular microscope. The COI gene was sequenced from one individual of each morphotype. Microscopic observation placed the samples into 15 morphotypes. DNA barcoding placed twelve morphotypes as Crustacea with sequence homologies from 72.21% to 99.21%. Intra-species genetic divergences between samples and reference species ranged between 0.9% and 31.9%, while genetic distance ranged from 0.0% to 17.80%. Intra-species genetic divergences ranged between 0.00% and 3.9%, while genetic distance ranged from 0.00% to 3.8%. The phylogenetic tree proved the monophyly between samples and reference species and showed clear separation among species. All parameters proved that nine morphotypes were identified into species level and were counted for five species. Three morphotypes were identified into the genus level and were counted for three genera. Eight species of crustacean larvae were successfully identified using the cytochrome c oxidase subunit 1 gene.KUSBIYANTO KUSBIYANTODIAN BHAGAWATIAgus NuryantoMBI & UNS Soloarticlebarcoding, crustacea, cytochrome c oxidase gene, larvae, species identificationBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	barcoding, crustacea, cytochrome c oxidase gene, larvae, species identification Biology (General) QH301-705.5
spellingShingle	barcoding, crustacea, cytochrome c oxidase gene, larvae, species identification Biology (General) QH301-705.5 KUSBIYANTO KUSBIYANTO DIAN BHAGAWATI Agus Nuryanto DNA barcoding of Crustacean larvae in the eastern areas of Segara Anakan Cilacap, Central Java Indonesia
description	Abstract. Kusbiyanto, Bhagawati D, Nuryanto A. 2020. DNA barcoding of crustacean larvae in Segara Anakan, Cilacap, Central Java, Indonesia using cytochrome c oxidase gene. Biodiversitas 21: 4878-4887. Species-level identification of crustacean larvae is challenging due to morphological constraints. DNA barcoding offers a precise method to solve the problems. That method has never been applied to crustacean larvae from the eastern of Segara Anakan, Cilacap, Central Java, Indonesia. This study aims to identify crustacean larvae in the eastern of Segara Anakan using the cytochrome c oxidase subunit I (COI) gene as a barcode marker. Larvae morphotypes were identified under a binocular microscope. The COI gene was sequenced from one individual of each morphotype. Microscopic observation placed the samples into 15 morphotypes. DNA barcoding placed twelve morphotypes as Crustacea with sequence homologies from 72.21% to 99.21%. Intra-species genetic divergences between samples and reference species ranged between 0.9% and 31.9%, while genetic distance ranged from 0.0% to 17.80%. Intra-species genetic divergences ranged between 0.00% and 3.9%, while genetic distance ranged from 0.00% to 3.8%. The phylogenetic tree proved the monophyly between samples and reference species and showed clear separation among species. All parameters proved that nine morphotypes were identified into species level and were counted for five species. Three morphotypes were identified into the genus level and were counted for three genera. Eight species of crustacean larvae were successfully identified using the cytochrome c oxidase subunit 1 gene.
format	article
author	KUSBIYANTO KUSBIYANTO DIAN BHAGAWATI Agus Nuryanto
author_facet	KUSBIYANTO KUSBIYANTO DIAN BHAGAWATI Agus Nuryanto
author_sort	KUSBIYANTO KUSBIYANTO
title	DNA barcoding of Crustacean larvae in the eastern areas of Segara Anakan Cilacap, Central Java Indonesia
title_short	DNA barcoding of Crustacean larvae in the eastern areas of Segara Anakan Cilacap, Central Java Indonesia
title_full	DNA barcoding of Crustacean larvae in the eastern areas of Segara Anakan Cilacap, Central Java Indonesia
title_fullStr	DNA barcoding of Crustacean larvae in the eastern areas of Segara Anakan Cilacap, Central Java Indonesia
title_full_unstemmed	DNA barcoding of Crustacean larvae in the eastern areas of Segara Anakan Cilacap, Central Java Indonesia
title_sort	dna barcoding of crustacean larvae in the eastern areas of segara anakan cilacap, central java indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/813b16d115f44241897c44707bc5849d
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DNA barcoding of Crustacean larvae in the eastern areas of Segara Anakan Cilacap, Central Java Indonesia

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