Printing of vessels for small functional tissues – a preliminary study

Vascularization of bioprinted constructs to ensure sufficient nutrient supply still remains to be a significant task in the tissue engineering community. In order to mimic functional tissue, it is necessary to be able to print vessels in various size scales, which places particularly high demands on...

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Autores principales:	Polley Christian, Kussauer Sophie, David Robert, Barkow Phillip, Mau Robert, Seitz Hermann
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2020
Materias:	3d printing hydrogels bioprinting vascularization Medicine R
Acceso en línea:	https://doaj.org/article/81498426a24d49d7ac25ae25f2a82dc6
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spelling	oai:doaj.org-article:81498426a24d49d7ac25ae25f2a82dc62021-12-05T14:10:43ZPrinting of vessels for small functional tissues – a preliminary study2364-550410.1515/cdbme-2020-3121https://doaj.org/article/81498426a24d49d7ac25ae25f2a82dc62020-09-01T00:00:00Zhttps://doi.org/10.1515/cdbme-2020-3121https://doaj.org/toc/2364-5504Vascularization of bioprinted constructs to ensure sufficient nutrient supply still remains to be a significant task in the tissue engineering community. In order to mimic functional tissue, it is necessary to be able to print vessels in various size scales, which places particularly high demands on the 3D printing technology and materials. In this preliminary study, we focused on the production of small hollow structures for the application in small functional units of living tissue. To fabricate hollow structures, the freeform reversible embedding of suspended hydrogels (FRESH) - method was utilized (Hinton et al.). A sodium alginate solution (5 % w/v) was used as a bioink. The scaffolds were fabricated with the Allevi 1 (Allevi Inc., PA, USA), a pneumatic extrusion-based bioprinter and plotted into a gelatine slurry serving as fugitive support. For first cell experiments, the bioink was loaded with immortalized mouse HL1-cells. A proof of concept could be shown since we were able to reliably create vessel-like structures with an inside diameter of 1.2 to 1.6 mm, a length of up to 8 mm and a wall thickness of 0.4 to 0.6 mm. In this study, the geometric requirements to print hollow structures for small functional tissues could be achieved. To expand the field of applications the resolution of the printing process has to be further improved. Moreover, the cell density should be increased to reach physiological cell numbers and extended with endothelial cells.Polley ChristianKussauer SophieDavid RobertBarkow PhillipMau RobertSeitz HermannDe Gruyterarticle3d printinghydrogelsbioprintingvascularizationMedicineRENCurrent Directions in Biomedical Engineering, Vol 6, Iss 3, Pp 469-472 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	3d printing hydrogels bioprinting vascularization Medicine R
spellingShingle	3d printing hydrogels bioprinting vascularization Medicine R Polley Christian Kussauer Sophie David Robert Barkow Phillip Mau Robert Seitz Hermann Printing of vessels for small functional tissues – a preliminary study
description	Vascularization of bioprinted constructs to ensure sufficient nutrient supply still remains to be a significant task in the tissue engineering community. In order to mimic functional tissue, it is necessary to be able to print vessels in various size scales, which places particularly high demands on the 3D printing technology and materials. In this preliminary study, we focused on the production of small hollow structures for the application in small functional units of living tissue. To fabricate hollow structures, the freeform reversible embedding of suspended hydrogels (FRESH) - method was utilized (Hinton et al.). A sodium alginate solution (5 % w/v) was used as a bioink. The scaffolds were fabricated with the Allevi 1 (Allevi Inc., PA, USA), a pneumatic extrusion-based bioprinter and plotted into a gelatine slurry serving as fugitive support. For first cell experiments, the bioink was loaded with immortalized mouse HL1-cells. A proof of concept could be shown since we were able to reliably create vessel-like structures with an inside diameter of 1.2 to 1.6 mm, a length of up to 8 mm and a wall thickness of 0.4 to 0.6 mm. In this study, the geometric requirements to print hollow structures for small functional tissues could be achieved. To expand the field of applications the resolution of the printing process has to be further improved. Moreover, the cell density should be increased to reach physiological cell numbers and extended with endothelial cells.
format	article
author	Polley Christian Kussauer Sophie David Robert Barkow Phillip Mau Robert Seitz Hermann
author_facet	Polley Christian Kussauer Sophie David Robert Barkow Phillip Mau Robert Seitz Hermann
author_sort	Polley Christian
title	Printing of vessels for small functional tissues – a preliminary study
title_short	Printing of vessels for small functional tissues – a preliminary study
title_full	Printing of vessels for small functional tissues – a preliminary study
title_fullStr	Printing of vessels for small functional tissues – a preliminary study
title_full_unstemmed	Printing of vessels for small functional tissues – a preliminary study
title_sort	printing of vessels for small functional tissues – a preliminary study
publisher	De Gruyter
publishDate	2020
url	https://doaj.org/article/81498426a24d49d7ac25ae25f2a82dc6
work_keys_str_mv	AT polleychristian printingofvesselsforsmallfunctionaltissuesapreliminarystudy AT kussauersophie printingofvesselsforsmallfunctionaltissuesapreliminarystudy AT davidrobert printingofvesselsforsmallfunctionaltissuesapreliminarystudy AT barkowphillip printingofvesselsforsmallfunctionaltissuesapreliminarystudy AT maurobert printingofvesselsforsmallfunctionaltissuesapreliminarystudy AT seitzhermann printingofvesselsforsmallfunctionaltissuesapreliminarystudy
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Printing of vessels for small functional tissues – a preliminary study

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