Genome-wide characterization of the hyperaccumulator Sedum alfredii F-box family under cadmium stress

Abstract The F-box genes, which form one of the largest gene families in plants, are vital for plant growth, development and stress response. However, F-box gene family in Sedum alfredii remains unknown. Comprehensive studies addressing their function responding to cadmium stress is still limited. I...

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Autores principales:	Zhuang Zhang, Wenmin Qiu, Wen Liu, Xiaojiao Han, Longhua Wu, Miao Yu, Xuelong Qiu, Zhengquan He, HaiYing Li, Renying Zhuo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/814c7225e06d42ebbc9b2866611fb62b
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spelling	oai:doaj.org-article:814c7225e06d42ebbc9b2866611fb62b2021-12-02T14:06:57ZGenome-wide characterization of the hyperaccumulator Sedum alfredii F-box family under cadmium stress10.1038/s41598-021-82690-72045-2322https://doaj.org/article/814c7225e06d42ebbc9b2866611fb62b2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82690-7https://doaj.org/toc/2045-2322Abstract The F-box genes, which form one of the largest gene families in plants, are vital for plant growth, development and stress response. However, F-box gene family in Sedum alfredii remains unknown. Comprehensive studies addressing their function responding to cadmium stress is still limited. In the present study, 193 members of the F-box gene (SaFbox) family were identified, which were classified into nine subfamilies. Most of the SaFboxs had highly conserved domain and motif. Various functionally related cis-elements involved in plant growth regulation, stress and hormone responses were located in the upstream regions of SaFbox genes. RNA-sequencing and co-expression network analysis revealed that the identified SaFbox genes would be involved in Cd stress. Expression analysis of 16 hub genes confirmed their transcription level in different tissues. Four hub genes (SaFbox40, SaFbox51, SaFbox136 and SaFbox170) were heterologously expressed in a Cd-sensitive yeast cell to assess their effects on Cd tolerance. The transgenic yeast cells carrying SaFbox40, SaFbox51, SaFbox136, or SaFbox170 were more sensitive and accumulated more cadmium under Cd stress than empty vector transformed control cells. Our results performed a comprehensive analysis of Fboxs in S. alfredii and identified their potential roles in Cd stress response.Zhuang ZhangWenmin QiuWen LiuXiaojiao HanLonghua WuMiao YuXuelong QiuZhengquan HeHaiYing LiRenying ZhuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhuang Zhang Wenmin Qiu Wen Liu Xiaojiao Han Longhua Wu Miao Yu Xuelong Qiu Zhengquan He HaiYing Li Renying Zhuo Genome-wide characterization of the hyperaccumulator Sedum alfredii F-box family under cadmium stress
description	Abstract The F-box genes, which form one of the largest gene families in plants, are vital for plant growth, development and stress response. However, F-box gene family in Sedum alfredii remains unknown. Comprehensive studies addressing their function responding to cadmium stress is still limited. In the present study, 193 members of the F-box gene (SaFbox) family were identified, which were classified into nine subfamilies. Most of the SaFboxs had highly conserved domain and motif. Various functionally related cis-elements involved in plant growth regulation, stress and hormone responses were located in the upstream regions of SaFbox genes. RNA-sequencing and co-expression network analysis revealed that the identified SaFbox genes would be involved in Cd stress. Expression analysis of 16 hub genes confirmed their transcription level in different tissues. Four hub genes (SaFbox40, SaFbox51, SaFbox136 and SaFbox170) were heterologously expressed in a Cd-sensitive yeast cell to assess their effects on Cd tolerance. The transgenic yeast cells carrying SaFbox40, SaFbox51, SaFbox136, or SaFbox170 were more sensitive and accumulated more cadmium under Cd stress than empty vector transformed control cells. Our results performed a comprehensive analysis of Fboxs in S. alfredii and identified their potential roles in Cd stress response.
format	article
author	Zhuang Zhang Wenmin Qiu Wen Liu Xiaojiao Han Longhua Wu Miao Yu Xuelong Qiu Zhengquan He HaiYing Li Renying Zhuo
author_facet	Zhuang Zhang Wenmin Qiu Wen Liu Xiaojiao Han Longhua Wu Miao Yu Xuelong Qiu Zhengquan He HaiYing Li Renying Zhuo
author_sort	Zhuang Zhang
title	Genome-wide characterization of the hyperaccumulator Sedum alfredii F-box family under cadmium stress
title_short	Genome-wide characterization of the hyperaccumulator Sedum alfredii F-box family under cadmium stress
title_full	Genome-wide characterization of the hyperaccumulator Sedum alfredii F-box family under cadmium stress
title_fullStr	Genome-wide characterization of the hyperaccumulator Sedum alfredii F-box family under cadmium stress
title_full_unstemmed	Genome-wide characterization of the hyperaccumulator Sedum alfredii F-box family under cadmium stress
title_sort	genome-wide characterization of the hyperaccumulator sedum alfredii f-box family under cadmium stress
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/814c7225e06d42ebbc9b2866611fb62b
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Genome-wide characterization of the hyperaccumulator Sedum alfredii F-box family under cadmium stress

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