Differences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are erased upon differentiation.

Little is known about differences between induced pluripotent stem cells produced from tissues originating from the same germ layer. We have generated human myoblast-derived iPS cells by retroviral transduction of human primary myoblasts with the OCT3/4, SOX2, KLF4 and MYC coding sequences and compa...

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Autores principales:	Iryna Pirozhkova, Ana Barat, Petr Dmitriev, Elena Kim, Thomas Robert, Justine Guégan, Chrystèle Bilhou-Nabera, Florence Busato, Jörg Tost, Gilles Carnac, Dalila Laoudj-Chenivesse, Marc Lipinski, Yegor Vassetzky
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/814da61bcb1446f3a61f5c3027b5edbe
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spelling	oai:doaj.org-article:814da61bcb1446f3a61f5c3027b5edbe2021-11-18T08:02:12ZDifferences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are erased upon differentiation.1932-620310.1371/journal.pone.0053033https://doaj.org/article/814da61bcb1446f3a61f5c3027b5edbe2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23326377/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Little is known about differences between induced pluripotent stem cells produced from tissues originating from the same germ layer. We have generated human myoblast-derived iPS cells by retroviral transduction of human primary myoblasts with the OCT3/4, SOX2, KLF4 and MYC coding sequences and compared them to iPS produced from human primary fibroblasts. When cultivated in vitro, these iPS cells proved similar to human embryonic stem cells in terms of morphology, expression of embryonic stemness markers and gene promoter methylation patterns. Embryonic bodies were derived that expressed endodermal, mesodermal as well as ectodermal markers. A comparative analysis of transcription patterns revealed significant differences in the gene expression pattern between myoblast- and fibroblast-derived iPS cells. However, these differences were reduced in the mesenchymal stem cells derived from the two iPS cell types were compared.Iryna PirozhkovaAna BaratPetr DmitrievElena KimThomas RobertJustine GuéganChrystèle Bilhou-NaberaFlorence BusatoJörg TostGilles CarnacDalila Laoudj-ChenivesseMarc LipinskiYegor VassetzkyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53033 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Iryna Pirozhkova Ana Barat Petr Dmitriev Elena Kim Thomas Robert Justine Guégan Chrystèle Bilhou-Nabera Florence Busato Jörg Tost Gilles Carnac Dalila Laoudj-Chenivesse Marc Lipinski Yegor Vassetzky Differences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are erased upon differentiation.
description	Little is known about differences between induced pluripotent stem cells produced from tissues originating from the same germ layer. We have generated human myoblast-derived iPS cells by retroviral transduction of human primary myoblasts with the OCT3/4, SOX2, KLF4 and MYC coding sequences and compared them to iPS produced from human primary fibroblasts. When cultivated in vitro, these iPS cells proved similar to human embryonic stem cells in terms of morphology, expression of embryonic stemness markers and gene promoter methylation patterns. Embryonic bodies were derived that expressed endodermal, mesodermal as well as ectodermal markers. A comparative analysis of transcription patterns revealed significant differences in the gene expression pattern between myoblast- and fibroblast-derived iPS cells. However, these differences were reduced in the mesenchymal stem cells derived from the two iPS cell types were compared.
format	article
author	Iryna Pirozhkova Ana Barat Petr Dmitriev Elena Kim Thomas Robert Justine Guégan Chrystèle Bilhou-Nabera Florence Busato Jörg Tost Gilles Carnac Dalila Laoudj-Chenivesse Marc Lipinski Yegor Vassetzky
author_facet	Iryna Pirozhkova Ana Barat Petr Dmitriev Elena Kim Thomas Robert Justine Guégan Chrystèle Bilhou-Nabera Florence Busato Jörg Tost Gilles Carnac Dalila Laoudj-Chenivesse Marc Lipinski Yegor Vassetzky
author_sort	Iryna Pirozhkova
title	Differences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are erased upon differentiation.
title_short	Differences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are erased upon differentiation.
title_full	Differences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are erased upon differentiation.
title_fullStr	Differences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are erased upon differentiation.
title_full_unstemmed	Differences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are erased upon differentiation.
title_sort	differences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are erased upon differentiation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/814da61bcb1446f3a61f5c3027b5edbe
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Differences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are erased upon differentiation.

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