Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma

Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma

Abstract Head and neck squamous cell carcinoma (HNSCC) represents a major health concern worldwide. We applied the matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) to analyze paired normal (N) and tumor (T) samples from head and neck squamous cell carcinoma as well...

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Autores principales: Li-Hsing Chi, Wei-Min Chang, Yu-Chan Chang, Yung-Chieh Chan, Chia-Chen Tai, Kam-Wing Leung, Chi-Long Chen, Alexander TH Wu, Tsung-Ching Lai, Yu-Chuan (Jack) Li, Michael Hsiao
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/814ef1814c074b55994e2c15199166ec
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spelling oai:doaj.org-article:814ef1814c074b55994e2c15199166ec2021-12-02T12:32:27ZGlobal Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma10.1038/s41598-017-09539-w2045-2322https://doaj.org/article/814ef1814c074b55994e2c15199166ec2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09539-whttps://doaj.org/toc/2045-2322Abstract Head and neck squamous cell carcinoma (HNSCC) represents a major health concern worldwide. We applied the matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) to analyze paired normal (N) and tumor (T) samples from head and neck squamous cell carcinoma as well as liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis in HNSCC cell lines to identify tumor-associated biomarkers. Our results showed a number of proteins found to be over-expressed in HNSCC. We identified thymosin beta-4 X-linked (TMSB4X) is one of the most significant candidate biomarkers. Higher TMSB4X expression in the tumor was found by N/T-paired HNSCC samples at both RNA and protein level. Overexpression of TMSB4X was found significantly associated with poor prognosis of overall survival (OS, P = 0.006) and recurrence-free survival (RFS, P = 0.013) in HNSCC patients. Silencing of TMSB4X expression in HNSCC cell line reduced the proliferation and invasion ability in vitro, as well as inhibited the cervical lymph node metastasis in vivo. Altogether, our global proteomics analysis identified that TMSB4X is a newly discovered biomarker in HNSCC whose functions resulted in enhanced proliferation and metastasis in vitro and in vivo. TMSB4X may be a potential therapeutic target for treating HNSCC patients.Li-Hsing ChiWei-Min ChangYu-Chan ChangYung-Chieh ChanChia-Chen TaiKam-Wing LeungChi-Long ChenAlexander TH WuTsung-Ching LaiYu-Chuan (Jack) LiMichael HsiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Li-Hsing Chi
Wei-Min Chang
Yu-Chan Chang
Yung-Chieh Chan
Chia-Chen Tai
Kam-Wing Leung
Chi-Long Chen
Alexander TH Wu
Tsung-Ching Lai
Yu-Chuan (Jack) Li
Michael Hsiao
Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma
description Abstract Head and neck squamous cell carcinoma (HNSCC) represents a major health concern worldwide. We applied the matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) to analyze paired normal (N) and tumor (T) samples from head and neck squamous cell carcinoma as well as liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis in HNSCC cell lines to identify tumor-associated biomarkers. Our results showed a number of proteins found to be over-expressed in HNSCC. We identified thymosin beta-4 X-linked (TMSB4X) is one of the most significant candidate biomarkers. Higher TMSB4X expression in the tumor was found by N/T-paired HNSCC samples at both RNA and protein level. Overexpression of TMSB4X was found significantly associated with poor prognosis of overall survival (OS, P = 0.006) and recurrence-free survival (RFS, P = 0.013) in HNSCC patients. Silencing of TMSB4X expression in HNSCC cell line reduced the proliferation and invasion ability in vitro, as well as inhibited the cervical lymph node metastasis in vivo. Altogether, our global proteomics analysis identified that TMSB4X is a newly discovered biomarker in HNSCC whose functions resulted in enhanced proliferation and metastasis in vitro and in vivo. TMSB4X may be a potential therapeutic target for treating HNSCC patients.
format article
author Li-Hsing Chi
Wei-Min Chang
Yu-Chan Chang
Yung-Chieh Chan
Chia-Chen Tai
Kam-Wing Leung
Chi-Long Chen
Alexander TH Wu
Tsung-Ching Lai
Yu-Chuan (Jack) Li
Michael Hsiao
author_facet Li-Hsing Chi
Wei-Min Chang
Yu-Chan Chang
Yung-Chieh Chan
Chia-Chen Tai
Kam-Wing Leung
Chi-Long Chen
Alexander TH Wu
Tsung-Ching Lai
Yu-Chuan (Jack) Li
Michael Hsiao
author_sort Li-Hsing Chi
title Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma
title_short Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma
title_full Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma
title_fullStr Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma
title_full_unstemmed Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma
title_sort global proteomics-based identification and validation of thymosin beta-4 x-linked as a prognostic marker for head and neck squamous cell carcinoma
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/814ef1814c074b55994e2c15199166ec
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