RNA-Binding Protein Expression Alters Upon Differentiation of Human B Cells and T Cells

B cells and T cells are key players in the defence against infections and malignancies. To exert their function, B cells and T cells differentiate into effector and memory cells. Tight regulation of these differentiation processes is key to prevent their malfunction, which can result in life-threate...

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Autores principales:	Nordin D. Zandhuis, Benoit P. Nicolet, Monika C. Wolkers
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	RNA binding protein T cells B cells B and T cell differentiation T cell cytotoxicity post transcriptional regulation (PTR) Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/815261d3e3124e25a3c438e0a91551a0
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spelling	oai:doaj.org-article:815261d3e3124e25a3c438e0a91551a02021-11-17T05:02:10ZRNA-Binding Protein Expression Alters Upon Differentiation of Human B Cells and T Cells1664-322410.3389/fimmu.2021.717324https://doaj.org/article/815261d3e3124e25a3c438e0a91551a02021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.717324/fullhttps://doaj.org/toc/1664-3224B cells and T cells are key players in the defence against infections and malignancies. To exert their function, B cells and T cells differentiate into effector and memory cells. Tight regulation of these differentiation processes is key to prevent their malfunction, which can result in life-threatening disease. Lymphocyte differentiation relies on the appropriate timing and dosage of regulatory molecules, and post-transcriptional gene regulation (PTR) is a key player herein. PTR includes the regulation through RNA-binding proteins (RBPs), which control the fate of RNA and its translation into proteins. To date, a comprehensive overview of the RBP expression throughout lymphocyte differentiation is lacking. Using transcriptome and proteome analyses, we here catalogued the RBP expression for human B cells and T cells. We observed that even though the overall RBP expression is conserved, the relative RBP expression is distinct between B cells and T cells. Differentiation into effector and memory cells alters the RBP expression, resulting into preferential expression of different classes of RBPs. For instance, whereas naive T cells express high levels of translation-regulating RBPs, effector T cells preferentially express RBPs that modulate mRNA stability. Lastly, we found that cytotoxic CD8+ and CD4+ T cells express a common RBP repertoire. Combined, our study reveals a cell type-specific and differentiation-dependent RBP expression landscape in human lymphocytes, which will help unravel the role of RBPs in lymphocyte function.Nordin D. ZandhuisNordin D. ZandhuisBenoit P. NicoletBenoit P. NicoletMonika C. WolkersMonika C. WolkersFrontiers Media S.A.articleRNA binding proteinT cellsB cellsB and T cell differentiationT cell cytotoxicitypost transcriptional regulation (PTR)Immunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	RNA binding protein T cells B cells B and T cell differentiation T cell cytotoxicity post transcriptional regulation (PTR) Immunologic diseases. Allergy RC581-607
spellingShingle	RNA binding protein T cells B cells B and T cell differentiation T cell cytotoxicity post transcriptional regulation (PTR) Immunologic diseases. Allergy RC581-607 Nordin D. Zandhuis Nordin D. Zandhuis Benoit P. Nicolet Benoit P. Nicolet Monika C. Wolkers Monika C. Wolkers RNA-Binding Protein Expression Alters Upon Differentiation of Human B Cells and T Cells
description	B cells and T cells are key players in the defence against infections and malignancies. To exert their function, B cells and T cells differentiate into effector and memory cells. Tight regulation of these differentiation processes is key to prevent their malfunction, which can result in life-threatening disease. Lymphocyte differentiation relies on the appropriate timing and dosage of regulatory molecules, and post-transcriptional gene regulation (PTR) is a key player herein. PTR includes the regulation through RNA-binding proteins (RBPs), which control the fate of RNA and its translation into proteins. To date, a comprehensive overview of the RBP expression throughout lymphocyte differentiation is lacking. Using transcriptome and proteome analyses, we here catalogued the RBP expression for human B cells and T cells. We observed that even though the overall RBP expression is conserved, the relative RBP expression is distinct between B cells and T cells. Differentiation into effector and memory cells alters the RBP expression, resulting into preferential expression of different classes of RBPs. For instance, whereas naive T cells express high levels of translation-regulating RBPs, effector T cells preferentially express RBPs that modulate mRNA stability. Lastly, we found that cytotoxic CD8+ and CD4+ T cells express a common RBP repertoire. Combined, our study reveals a cell type-specific and differentiation-dependent RBP expression landscape in human lymphocytes, which will help unravel the role of RBPs in lymphocyte function.
format	article
author	Nordin D. Zandhuis Nordin D. Zandhuis Benoit P. Nicolet Benoit P. Nicolet Monika C. Wolkers Monika C. Wolkers
author_facet	Nordin D. Zandhuis Nordin D. Zandhuis Benoit P. Nicolet Benoit P. Nicolet Monika C. Wolkers Monika C. Wolkers
author_sort	Nordin D. Zandhuis
title	RNA-Binding Protein Expression Alters Upon Differentiation of Human B Cells and T Cells
title_short	RNA-Binding Protein Expression Alters Upon Differentiation of Human B Cells and T Cells
title_full	RNA-Binding Protein Expression Alters Upon Differentiation of Human B Cells and T Cells
title_fullStr	RNA-Binding Protein Expression Alters Upon Differentiation of Human B Cells and T Cells
title_full_unstemmed	RNA-Binding Protein Expression Alters Upon Differentiation of Human B Cells and T Cells
title_sort	rna-binding protein expression alters upon differentiation of human b cells and t cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/815261d3e3124e25a3c438e0a91551a0
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RNA-Binding Protein Expression Alters Upon Differentiation of Human B Cells and T Cells

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