Flavonoid/Polyphenol Ratio in <i>Mauritia flexuosa</i> and <i>Theobroma grandiflorum</i> as an Indicator of Effective Antioxidant Action
Studies on polyphenols and flavonoids in natural products reveal benefits in the prevention of multiple diseases. Proper extraction, treatment of extracts, and quantification of polyphenols and flavonoids demand attention from the scientific community in order to report more specific biological acti...
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oai:doaj.org-article:81584f3778c64696832727468d476bdd2021-11-11T18:26:48ZFlavonoid/Polyphenol Ratio in <i>Mauritia flexuosa</i> and <i>Theobroma grandiflorum</i> as an Indicator of Effective Antioxidant Action10.3390/molecules262164311420-3049https://doaj.org/article/81584f3778c64696832727468d476bdd2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6431https://doaj.org/toc/1420-3049Studies on polyphenols and flavonoids in natural products reveal benefits in the prevention of multiple diseases. Proper extraction, treatment of extracts, and quantification of polyphenols and flavonoids demand attention from the scientific community in order to report more specific biological action. Total polyphenol content (TPC) and total flavonoid content (TFC) (measured at three different times) of ethanol, methanol and acetone extracts of <i>Mauritia flexuosa</i> (aguaje) and <i>Theobroma grandiflorum</i> (copoazú) fresh pulp, from the Colombian Amazon region, were evaluated with the purpose of focusing in the polyphenol/flavonoid proportion and its effective antioxidant activity. This objective could help to explain specific flavonoid biological action based on higher flavonoid proportion rather than higher total polyphenol content. Differences in extracting solvents resulted in statistically significant different yields; the highest TPC was observed with acetone 70% in <i>Mauritia flexuosa</i> and ethanol 80% for <i>T. grandiflorum.</i> The best flavonoid/polyphenol ratio in <i>M. flexuosa</i> was about 1:2.4 and 1:12.8 in <i>T. grandiflorum</i> and the antioxidant efficacy was proportionally higher for flavonoids extracted from <i>T. grandiflorum</i>. HPLC analysis revealed 54 µg/g of the flavonoid kaempferol in <i>M. Flexuosa</i> and 29 µg/g in <i>T. grandiflorum</i>. Further studies evaluating this proportionality, in seeds or peel of fruits, as well as, other specific biological activities, could help to understand the detailed flavonoid action without focusing on the high total polyphenol content.Juan Carlos Carmona-HernandezMai LeAna María Idárraga-MejíaClara Helena González-CorreaMDPI AGarticlepolyphenolsflavonoidsantioxidant activity<i>Mauritia flexuosa</i> (aguaje)<i>Theobroma grandiflorum</i> (copoazú)Organic chemistryQD241-441ENMolecules, Vol 26, Iss 6431, p 6431 (2021) |
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polyphenols flavonoids antioxidant activity <i>Mauritia flexuosa</i> (aguaje) <i>Theobroma grandiflorum</i> (copoazú) Organic chemistry QD241-441 |
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polyphenols flavonoids antioxidant activity <i>Mauritia flexuosa</i> (aguaje) <i>Theobroma grandiflorum</i> (copoazú) Organic chemistry QD241-441 Juan Carlos Carmona-Hernandez Mai Le Ana María Idárraga-Mejía Clara Helena González-Correa Flavonoid/Polyphenol Ratio in <i>Mauritia flexuosa</i> and <i>Theobroma grandiflorum</i> as an Indicator of Effective Antioxidant Action |
description |
Studies on polyphenols and flavonoids in natural products reveal benefits in the prevention of multiple diseases. Proper extraction, treatment of extracts, and quantification of polyphenols and flavonoids demand attention from the scientific community in order to report more specific biological action. Total polyphenol content (TPC) and total flavonoid content (TFC) (measured at three different times) of ethanol, methanol and acetone extracts of <i>Mauritia flexuosa</i> (aguaje) and <i>Theobroma grandiflorum</i> (copoazú) fresh pulp, from the Colombian Amazon region, were evaluated with the purpose of focusing in the polyphenol/flavonoid proportion and its effective antioxidant activity. This objective could help to explain specific flavonoid biological action based on higher flavonoid proportion rather than higher total polyphenol content. Differences in extracting solvents resulted in statistically significant different yields; the highest TPC was observed with acetone 70% in <i>Mauritia flexuosa</i> and ethanol 80% for <i>T. grandiflorum.</i> The best flavonoid/polyphenol ratio in <i>M. flexuosa</i> was about 1:2.4 and 1:12.8 in <i>T. grandiflorum</i> and the antioxidant efficacy was proportionally higher for flavonoids extracted from <i>T. grandiflorum</i>. HPLC analysis revealed 54 µg/g of the flavonoid kaempferol in <i>M. Flexuosa</i> and 29 µg/g in <i>T. grandiflorum</i>. Further studies evaluating this proportionality, in seeds or peel of fruits, as well as, other specific biological activities, could help to understand the detailed flavonoid action without focusing on the high total polyphenol content. |
format |
article |
author |
Juan Carlos Carmona-Hernandez Mai Le Ana María Idárraga-Mejía Clara Helena González-Correa |
author_facet |
Juan Carlos Carmona-Hernandez Mai Le Ana María Idárraga-Mejía Clara Helena González-Correa |
author_sort |
Juan Carlos Carmona-Hernandez |
title |
Flavonoid/Polyphenol Ratio in <i>Mauritia flexuosa</i> and <i>Theobroma grandiflorum</i> as an Indicator of Effective Antioxidant Action |
title_short |
Flavonoid/Polyphenol Ratio in <i>Mauritia flexuosa</i> and <i>Theobroma grandiflorum</i> as an Indicator of Effective Antioxidant Action |
title_full |
Flavonoid/Polyphenol Ratio in <i>Mauritia flexuosa</i> and <i>Theobroma grandiflorum</i> as an Indicator of Effective Antioxidant Action |
title_fullStr |
Flavonoid/Polyphenol Ratio in <i>Mauritia flexuosa</i> and <i>Theobroma grandiflorum</i> as an Indicator of Effective Antioxidant Action |
title_full_unstemmed |
Flavonoid/Polyphenol Ratio in <i>Mauritia flexuosa</i> and <i>Theobroma grandiflorum</i> as an Indicator of Effective Antioxidant Action |
title_sort |
flavonoid/polyphenol ratio in <i>mauritia flexuosa</i> and <i>theobroma grandiflorum</i> as an indicator of effective antioxidant action |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doaj.org/article/81584f3778c64696832727468d476bdd |
work_keys_str_mv |
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