A Novel Serum tsRNA for Diagnosis and Prediction of Nephritis in SLE
ObjectiveDysregulation of transfer RNA (tRNA)-derived small noncoding RNA (tsRNA) signatures in human serum has been found in various diseases. Here, we determine whether the signatures of tsRNAs in serum can serve as biomarkers for diagnosis or prognosis of systemic lupus erythematosus (SLE).Method...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/815d1593b4e649dc8fe719084194c902 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:815d1593b4e649dc8fe719084194c902 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:815d1593b4e649dc8fe719084194c9022021-11-11T12:28:26ZA Novel Serum tsRNA for Diagnosis and Prediction of Nephritis in SLE1664-322410.3389/fimmu.2021.735105https://doaj.org/article/815d1593b4e649dc8fe719084194c9022021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.735105/fullhttps://doaj.org/toc/1664-3224ObjectiveDysregulation of transfer RNA (tRNA)-derived small noncoding RNA (tsRNA) signatures in human serum has been found in various diseases. Here, we determine whether the signatures of tsRNAs in serum can serve as biomarkers for diagnosis or prognosis of systemic lupus erythematosus (SLE).MethodsInitially, small RNA sequencing was employed for the screening serum tsRNAs obtained from SLE patients, followed by validation with TaqMan probe-based quantitative reverse transcription-PCR (RT-PCR) assay. Receiver operating characteristic (ROC) curve analysis was used to assess the diagnostic efficacy. The biological functions of tsRNAs were identified by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) assay.ResultsWe first analyzed tsRNA signatures in SLE serum and identified that tRF-His-GTG-1 was significantly upregulated in SLE serum. The combination of tRF-His-GTG-1 and anti-dsDNA could serve as biomarkers for diagnosing SLE with a high area under the curve (AUC) of 0.95 (95% CI = 0.92–0.99), sensitivity (83.72%), and specificity (94.19%). Importantly, the noninvasive serum tRF-His-GTG-1 could also be used to distinguish SLE with LN or SLE without LN with AUC of 0.81 (95% CI, 0.73–0.88) and performance (sensitivity 66.27%, specificity 96.15%). Moreover, the serum tsRNA is mainly secreted via exosome and can directly target signaling molecules that play crucial roles in regulating the immune system.ConclusionIn this study, it has been demonstrated for the first time that serum tsRNAs can be employed as noninvasive biomarkers for the efficient diagnosis and prediction of nephritis in SLE.Ping YangXiaoshan ZhangShanshan ChenYue TaoMingzhe NingYijia ZhuJun LiangWei KongBo ShiZhiyang LiHan ShenYanbo WangFrontiers Media S.A.articletsRNASLELNbiomarkerncRNA (non coding RNA)diagnosisImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
tsRNA SLE LN biomarker ncRNA (non coding RNA) diagnosis Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
tsRNA SLE LN biomarker ncRNA (non coding RNA) diagnosis Immunologic diseases. Allergy RC581-607 Ping Yang Xiaoshan Zhang Shanshan Chen Yue Tao Mingzhe Ning Yijia Zhu Jun Liang Wei Kong Bo Shi Zhiyang Li Han Shen Yanbo Wang A Novel Serum tsRNA for Diagnosis and Prediction of Nephritis in SLE |
| description |
ObjectiveDysregulation of transfer RNA (tRNA)-derived small noncoding RNA (tsRNA) signatures in human serum has been found in various diseases. Here, we determine whether the signatures of tsRNAs in serum can serve as biomarkers for diagnosis or prognosis of systemic lupus erythematosus (SLE).MethodsInitially, small RNA sequencing was employed for the screening serum tsRNAs obtained from SLE patients, followed by validation with TaqMan probe-based quantitative reverse transcription-PCR (RT-PCR) assay. Receiver operating characteristic (ROC) curve analysis was used to assess the diagnostic efficacy. The biological functions of tsRNAs were identified by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) assay.ResultsWe first analyzed tsRNA signatures in SLE serum and identified that tRF-His-GTG-1 was significantly upregulated in SLE serum. The combination of tRF-His-GTG-1 and anti-dsDNA could serve as biomarkers for diagnosing SLE with a high area under the curve (AUC) of 0.95 (95% CI = 0.92–0.99), sensitivity (83.72%), and specificity (94.19%). Importantly, the noninvasive serum tRF-His-GTG-1 could also be used to distinguish SLE with LN or SLE without LN with AUC of 0.81 (95% CI, 0.73–0.88) and performance (sensitivity 66.27%, specificity 96.15%). Moreover, the serum tsRNA is mainly secreted via exosome and can directly target signaling molecules that play crucial roles in regulating the immune system.ConclusionIn this study, it has been demonstrated for the first time that serum tsRNAs can be employed as noninvasive biomarkers for the efficient diagnosis and prediction of nephritis in SLE. |
| format |
article |
| author |
Ping Yang Xiaoshan Zhang Shanshan Chen Yue Tao Mingzhe Ning Yijia Zhu Jun Liang Wei Kong Bo Shi Zhiyang Li Han Shen Yanbo Wang |
| author_facet |
Ping Yang Xiaoshan Zhang Shanshan Chen Yue Tao Mingzhe Ning Yijia Zhu Jun Liang Wei Kong Bo Shi Zhiyang Li Han Shen Yanbo Wang |
| author_sort |
Ping Yang |
| title |
A Novel Serum tsRNA for Diagnosis and Prediction of Nephritis in SLE |
| title_short |
A Novel Serum tsRNA for Diagnosis and Prediction of Nephritis in SLE |
| title_full |
A Novel Serum tsRNA for Diagnosis and Prediction of Nephritis in SLE |
| title_fullStr |
A Novel Serum tsRNA for Diagnosis and Prediction of Nephritis in SLE |
| title_full_unstemmed |
A Novel Serum tsRNA for Diagnosis and Prediction of Nephritis in SLE |
| title_sort |
novel serum tsrna for diagnosis and prediction of nephritis in sle |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/815d1593b4e649dc8fe719084194c902 |
| work_keys_str_mv |
AT pingyang anovelserumtsrnafordiagnosisandpredictionofnephritisinsle AT xiaoshanzhang anovelserumtsrnafordiagnosisandpredictionofnephritisinsle AT shanshanchen anovelserumtsrnafordiagnosisandpredictionofnephritisinsle AT yuetao anovelserumtsrnafordiagnosisandpredictionofnephritisinsle AT mingzhening anovelserumtsrnafordiagnosisandpredictionofnephritisinsle AT yijiazhu anovelserumtsrnafordiagnosisandpredictionofnephritisinsle AT junliang anovelserumtsrnafordiagnosisandpredictionofnephritisinsle AT weikong anovelserumtsrnafordiagnosisandpredictionofnephritisinsle AT boshi anovelserumtsrnafordiagnosisandpredictionofnephritisinsle AT zhiyangli anovelserumtsrnafordiagnosisandpredictionofnephritisinsle AT hanshen anovelserumtsrnafordiagnosisandpredictionofnephritisinsle AT yanbowang anovelserumtsrnafordiagnosisandpredictionofnephritisinsle AT pingyang novelserumtsrnafordiagnosisandpredictionofnephritisinsle AT xiaoshanzhang novelserumtsrnafordiagnosisandpredictionofnephritisinsle AT shanshanchen novelserumtsrnafordiagnosisandpredictionofnephritisinsle AT yuetao novelserumtsrnafordiagnosisandpredictionofnephritisinsle AT mingzhening novelserumtsrnafordiagnosisandpredictionofnephritisinsle AT yijiazhu novelserumtsrnafordiagnosisandpredictionofnephritisinsle AT junliang novelserumtsrnafordiagnosisandpredictionofnephritisinsle AT weikong novelserumtsrnafordiagnosisandpredictionofnephritisinsle AT boshi novelserumtsrnafordiagnosisandpredictionofnephritisinsle AT zhiyangli novelserumtsrnafordiagnosisandpredictionofnephritisinsle AT hanshen novelserumtsrnafordiagnosisandpredictionofnephritisinsle AT yanbowang novelserumtsrnafordiagnosisandpredictionofnephritisinsle |
| _version_ |
1718439050686234624 |