Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs

β-lactam antibiotics (BLs) are the drugs most frequently involved in drug hypersensitivity reactions. However, current in vitro diagnostic tests have limited sensitivity, partly due to a poor understanding of in vivo drug–protein conjugates that both induce the reactions and are immunologically reco...

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Autores principales:	Violeta Gil-Ocaña, Isabel M. Jimenez, Cristobalina Mayorga, Inmaculada Doña, Jose Antonio Céspedes, Maria I. Montañez, Yolanda Vida, Maria J. Torres, Ezequiel Perez-Inestrosa
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	dendrimeric antigen silica nanoparticles biepitope nanocomposites diagnostic test drug allergy penicillins Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/817313aa60f94f45a24d2100eb30b59e
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spelling	oai:doaj.org-article:817313aa60f94f45a24d2100eb30b59e2021-12-03T06:21:56ZMultiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs1664-322410.3389/fimmu.2021.750109https://doaj.org/article/817313aa60f94f45a24d2100eb30b59e2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.750109/fullhttps://doaj.org/toc/1664-3224β-lactam antibiotics (BLs) are the drugs most frequently involved in drug hypersensitivity reactions. However, current in vitro diagnostic tests have limited sensitivity, partly due to a poor understanding of in vivo drug–protein conjugates that both induce the reactions and are immunologically recognized. Dendrimeric Antigen-Silica particle composites (DeAn@SiO2), consisting on nanoparticles decorated with BL-DeAns are promising candidates for improving the in vitro clinical diagnostic practice. In this nano-inspired system biology, the synthetic dendrimer plays the role of the natural carrier protein, emulating its haptenation by drugs and amplifying the multivalence. Herein, we present the design and synthesis of new multivalent mono- and bi-epitope DeAn@SiO2, using amoxicillin and/or benzylpenicillin allergenic determinants as ligands. The homogeneous composition of nanoparticles provides high reproducibility and quality, which is critical for in vitro applications. The suitable functionalization of nanoparticles allows the anchoring of DeAn, minimizing the nonspecific interactions and facilitating the effective exposure to specific IgE; while the larger interaction area increments the likelihood of capturing specific IgE. This achievement is particularly important for improving sensitivity of current immunoassays since IgE levels in BL allergic patients are very low. Our data suggest that these new nano-based platforms provide a suitable tool for testing IgE recognition to more than one BL simultaneously. Immunochemical studies evidence that mono and bi-epitope DeAn@SiO2 composites could potentially allow the diagnosis of patients allergic to any of these drugs with a single test. These organic–inorganic hybrid materials represent the basis for the development of a single screening for BL-allergies.Violeta Gil-OcañaVioleta Gil-OcañaIsabel M. JimenezIsabel M. JimenezCristobalina MayorgaCristobalina MayorgaCristobalina MayorgaInmaculada DoñaInmaculada DoñaJose Antonio CéspedesMaria I. MontañezMaria I. MontañezYolanda VidaYolanda VidaMaria J. TorresMaria J. TorresMaria J. TorresMaria J. TorresEzequiel Perez-InestrosaEzequiel Perez-InestrosaFrontiers Media S.A.articledendrimeric antigensilica nanoparticlesbiepitope nanocompositesdiagnostic testdrug allergypenicillinsImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dendrimeric antigen silica nanoparticles biepitope nanocomposites diagnostic test drug allergy penicillins Immunologic diseases. Allergy RC581-607
spellingShingle	dendrimeric antigen silica nanoparticles biepitope nanocomposites diagnostic test drug allergy penicillins Immunologic diseases. Allergy RC581-607 Violeta Gil-Ocaña Violeta Gil-Ocaña Isabel M. Jimenez Isabel M. Jimenez Cristobalina Mayorga Cristobalina Mayorga Cristobalina Mayorga Inmaculada Doña Inmaculada Doña Jose Antonio Céspedes Maria I. Montañez Maria I. Montañez Yolanda Vida Yolanda Vida Maria J. Torres Maria J. Torres Maria J. Torres Maria J. Torres Ezequiel Perez-Inestrosa Ezequiel Perez-Inestrosa Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs
description	β-lactam antibiotics (BLs) are the drugs most frequently involved in drug hypersensitivity reactions. However, current in vitro diagnostic tests have limited sensitivity, partly due to a poor understanding of in vivo drug–protein conjugates that both induce the reactions and are immunologically recognized. Dendrimeric Antigen-Silica particle composites (DeAn@SiO2), consisting on nanoparticles decorated with BL-DeAns are promising candidates for improving the in vitro clinical diagnostic practice. In this nano-inspired system biology, the synthetic dendrimer plays the role of the natural carrier protein, emulating its haptenation by drugs and amplifying the multivalence. Herein, we present the design and synthesis of new multivalent mono- and bi-epitope DeAn@SiO2, using amoxicillin and/or benzylpenicillin allergenic determinants as ligands. The homogeneous composition of nanoparticles provides high reproducibility and quality, which is critical for in vitro applications. The suitable functionalization of nanoparticles allows the anchoring of DeAn, minimizing the nonspecific interactions and facilitating the effective exposure to specific IgE; while the larger interaction area increments the likelihood of capturing specific IgE. This achievement is particularly important for improving sensitivity of current immunoassays since IgE levels in BL allergic patients are very low. Our data suggest that these new nano-based platforms provide a suitable tool for testing IgE recognition to more than one BL simultaneously. Immunochemical studies evidence that mono and bi-epitope DeAn@SiO2 composites could potentially allow the diagnosis of patients allergic to any of these drugs with a single test. These organic–inorganic hybrid materials represent the basis for the development of a single screening for BL-allergies.
format	article
author	Violeta Gil-Ocaña Violeta Gil-Ocaña Isabel M. Jimenez Isabel M. Jimenez Cristobalina Mayorga Cristobalina Mayorga Cristobalina Mayorga Inmaculada Doña Inmaculada Doña Jose Antonio Céspedes Maria I. Montañez Maria I. Montañez Yolanda Vida Yolanda Vida Maria J. Torres Maria J. Torres Maria J. Torres Maria J. Torres Ezequiel Perez-Inestrosa Ezequiel Perez-Inestrosa
author_facet	Violeta Gil-Ocaña Violeta Gil-Ocaña Isabel M. Jimenez Isabel M. Jimenez Cristobalina Mayorga Cristobalina Mayorga Cristobalina Mayorga Inmaculada Doña Inmaculada Doña Jose Antonio Céspedes Maria I. Montañez Maria I. Montañez Yolanda Vida Yolanda Vida Maria J. Torres Maria J. Torres Maria J. Torres Maria J. Torres Ezequiel Perez-Inestrosa Ezequiel Perez-Inestrosa
author_sort	Violeta Gil-Ocaña
title	Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs
title_short	Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs
title_full	Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs
title_fullStr	Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs
title_full_unstemmed	Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs
title_sort	multiepitope dendrimeric antigen-silica particle composites as nano-based platforms for specific recognition of iges
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/817313aa60f94f45a24d2100eb30b59e
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Multiepitope Dendrimeric Antigen-Silica Particle Composites as Nano-Based Platforms for Specific Recognition of IgEs

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