Apoptosis Enhances the Replication of Human Coronavirus OC43

Human coronavirus OC43 (HCoV-OC43) is one of the coronaviruses causing a mild common cold, but few studies have been made on this strain. Here, we identified the molecular mechanisms involved in HCoV-OC43-induced apoptosis and its implications for viral reproduction in Vero cells and MRC-5 cells. HC...

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Autores principales:	Sony Maharjan, Mijeong Kang, Jinsoo Kim, Dongbum Kim, Sangkyu Park, Minyoung Kim, Kyeongbin Baek, Younghee Lee, Hyung-Joo Kwon
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	HCoV-OC43 apoptosis replication Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8178a3228f014dd4803338395d1a6b55
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spelling	oai:doaj.org-article:8178a3228f014dd4803338395d1a6b552021-11-25T19:13:16ZApoptosis Enhances the Replication of Human Coronavirus OC4310.3390/v131121991999-4915https://doaj.org/article/8178a3228f014dd4803338395d1a6b552021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2199https://doaj.org/toc/1999-4915Human coronavirus OC43 (HCoV-OC43) is one of the coronaviruses causing a mild common cold, but few studies have been made on this strain. Here, we identified the molecular mechanisms involved in HCoV-OC43-induced apoptosis and its implications for viral reproduction in Vero cells and MRC-5 cells. HCoV-OC43 infection induced apoptosis that was accompanied by cleavage of caspase-3 and PARP, degradation of cyclin D1, and cell cycle arrest at S and G2M phases. Dephosphorylation of STAT1 and STAT3, induced by HCoV-OC43 infection, was also associated with HCoV-OC43-mediated apoptosis. The pan-caspase inhibitor effectively prevented HCoV-OC43-induced apoptosis and reduced viral replication, suggesting that apoptosis contributes to viral replication. Collectively our results indicate that HCoV-OC43 induces caspase-dependent apoptosis to promote viral replication in Vero cells and MRC-5 cells.Sony MaharjanMijeong KangJinsoo KimDongbum KimSangkyu ParkMinyoung KimKyeongbin BaekYounghee LeeHyung-Joo KwonMDPI AGarticleHCoV-OC43apoptosisreplicationMicrobiologyQR1-502ENViruses, Vol 13, Iss 2199, p 2199 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HCoV-OC43 apoptosis replication Microbiology QR1-502
spellingShingle	HCoV-OC43 apoptosis replication Microbiology QR1-502 Sony Maharjan Mijeong Kang Jinsoo Kim Dongbum Kim Sangkyu Park Minyoung Kim Kyeongbin Baek Younghee Lee Hyung-Joo Kwon Apoptosis Enhances the Replication of Human Coronavirus OC43
description	Human coronavirus OC43 (HCoV-OC43) is one of the coronaviruses causing a mild common cold, but few studies have been made on this strain. Here, we identified the molecular mechanisms involved in HCoV-OC43-induced apoptosis and its implications for viral reproduction in Vero cells and MRC-5 cells. HCoV-OC43 infection induced apoptosis that was accompanied by cleavage of caspase-3 and PARP, degradation of cyclin D1, and cell cycle arrest at S and G2M phases. Dephosphorylation of STAT1 and STAT3, induced by HCoV-OC43 infection, was also associated with HCoV-OC43-mediated apoptosis. The pan-caspase inhibitor effectively prevented HCoV-OC43-induced apoptosis and reduced viral replication, suggesting that apoptosis contributes to viral replication. Collectively our results indicate that HCoV-OC43 induces caspase-dependent apoptosis to promote viral replication in Vero cells and MRC-5 cells.
format	article
author	Sony Maharjan Mijeong Kang Jinsoo Kim Dongbum Kim Sangkyu Park Minyoung Kim Kyeongbin Baek Younghee Lee Hyung-Joo Kwon
author_facet	Sony Maharjan Mijeong Kang Jinsoo Kim Dongbum Kim Sangkyu Park Minyoung Kim Kyeongbin Baek Younghee Lee Hyung-Joo Kwon
author_sort	Sony Maharjan
title	Apoptosis Enhances the Replication of Human Coronavirus OC43
title_short	Apoptosis Enhances the Replication of Human Coronavirus OC43
title_full	Apoptosis Enhances the Replication of Human Coronavirus OC43
title_fullStr	Apoptosis Enhances the Replication of Human Coronavirus OC43
title_full_unstemmed	Apoptosis Enhances the Replication of Human Coronavirus OC43
title_sort	apoptosis enhances the replication of human coronavirus oc43
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8178a3228f014dd4803338395d1a6b55
work_keys_str_mv	AT sonymaharjan apoptosisenhancesthereplicationofhumancoronavirusoc43 AT mijeongkang apoptosisenhancesthereplicationofhumancoronavirusoc43 AT jinsookim apoptosisenhancesthereplicationofhumancoronavirusoc43 AT dongbumkim apoptosisenhancesthereplicationofhumancoronavirusoc43 AT sangkyupark apoptosisenhancesthereplicationofhumancoronavirusoc43 AT minyoungkim apoptosisenhancesthereplicationofhumancoronavirusoc43 AT kyeongbinbaek apoptosisenhancesthereplicationofhumancoronavirusoc43 AT youngheelee apoptosisenhancesthereplicationofhumancoronavirusoc43 AT hyungjookwon apoptosisenhancesthereplicationofhumancoronavirusoc43
_version_	1718410166878076928

Apoptosis Enhances the Replication of Human Coronavirus OC43

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