The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR

The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR

Abstract Coptidis Rhizoma binds to the membrane receptors on hPDLSC/CMC, and the active ingredient Berberine (BER) that can be extracted from it may promote the proliferation and osteogenesis of periodontal ligament stem cells (hPDLSC). The membrane receptor that binds with BER on the cell surface o...

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Autores principales: Jin Liu, Xiaodan Zhao, Dandan Pei, Guo Sun, Ye Li, Chunhui Zhu, Cui Qiang, Junyi Sun, Jianfeng Shi, Yan Dong, Jianzhong Gou, Sicen Wang, Ang Li
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Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/817ba142af204852a14af24899beff10
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spelling oai:doaj.org-article:817ba142af204852a14af24899beff102021-12-02T15:09:04ZThe promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR10.1038/s41598-018-21116-32045-2322https://doaj.org/article/817ba142af204852a14af24899beff102018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21116-3https://doaj.org/toc/2045-2322Abstract Coptidis Rhizoma binds to the membrane receptors on hPDLSC/CMC, and the active ingredient Berberine (BER) that can be extracted from it may promote the proliferation and osteogenesis of periodontal ligament stem cells (hPDLSC). The membrane receptor that binds with BER on the cell surface of hPDLSC, the mechanism of direct interaction between BER and hPDLSC, and the related signal pathway are not yet clear. In this research, EGFR was screened as the affinity membrane receptor between BER and hPDLSC, through retention on CMC, competition with BER and by using a molecular docking simulation score. At the same time, the MAPK PCR Array was selected to screen the target genes that changed when hPDLSC was simulated by BER. In conclusion, BER may bind to EGFR on the cell membrane of hPDLSC so the intracellular ERK signalling pathways activate, and nuclear-related genes of FOS change, resulting in the effect of osteogenesis on PDLSC.Jin LiuXiaodan ZhaoDandan PeiGuo SunYe LiChunhui ZhuCui QiangJunyi SunJianfeng ShiYan DongJianzhong GouSicen WangAng LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jin Liu
Xiaodan Zhao
Dandan Pei
Guo Sun
Ye Li
Chunhui Zhu
Cui Qiang
Junyi Sun
Jianfeng Shi
Yan Dong
Jianzhong Gou
Sicen Wang
Ang Li
The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR
description Abstract Coptidis Rhizoma binds to the membrane receptors on hPDLSC/CMC, and the active ingredient Berberine (BER) that can be extracted from it may promote the proliferation and osteogenesis of periodontal ligament stem cells (hPDLSC). The membrane receptor that binds with BER on the cell surface of hPDLSC, the mechanism of direct interaction between BER and hPDLSC, and the related signal pathway are not yet clear. In this research, EGFR was screened as the affinity membrane receptor between BER and hPDLSC, through retention on CMC, competition with BER and by using a molecular docking simulation score. At the same time, the MAPK PCR Array was selected to screen the target genes that changed when hPDLSC was simulated by BER. In conclusion, BER may bind to EGFR on the cell membrane of hPDLSC so the intracellular ERK signalling pathways activate, and nuclear-related genes of FOS change, resulting in the effect of osteogenesis on PDLSC.
format article
author Jin Liu
Xiaodan Zhao
Dandan Pei
Guo Sun
Ye Li
Chunhui Zhu
Cui Qiang
Junyi Sun
Jianfeng Shi
Yan Dong
Jianzhong Gou
Sicen Wang
Ang Li
author_facet Jin Liu
Xiaodan Zhao
Dandan Pei
Guo Sun
Ye Li
Chunhui Zhu
Cui Qiang
Junyi Sun
Jianfeng Shi
Yan Dong
Jianzhong Gou
Sicen Wang
Ang Li
author_sort Jin Liu
title The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR
title_short The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR
title_full The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR
title_fullStr The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR
title_full_unstemmed The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR
title_sort promotion function of berberine for osteogenic differentiation of human periodontal ligament stem cells via erk-fos pathway mediated by egfr
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/817ba142af204852a14af24899beff10
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