Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.

Osteoarthritis (OA) as the main degenerative disease of articular cartilage in joints is accompanied by structural and compositional changes in the tissue. Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in wa...

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Autores principales:	Andrea Crețu, Carlos Mattea, Siegfried Stapf
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/818ec6e728c34bd2bfa9056f55f7587e
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spelling	oai:doaj.org-article:818ec6e728c34bd2bfa9056f55f7587e2021-12-02T20:17:35ZLow-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.1932-620310.1371/journal.pone.0256177https://doaj.org/article/818ec6e728c34bd2bfa9056f55f7587e2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256177https://doaj.org/toc/1932-6203Osteoarthritis (OA) as the main degenerative disease of articular cartilage in joints is accompanied by structural and compositional changes in the tissue. Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage. NMR investigations of cartilage generally address only the relaxation properties of water. In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA. Two signal components were identified in all measurements, characterized by very different T2 which suggests liquid-like and solid-like dynamics. These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water. The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.Andrea CrețuCarlos MatteaSiegfried StapfPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256177 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrea Crețu Carlos Mattea Siegfried Stapf Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
description	Osteoarthritis (OA) as the main degenerative disease of articular cartilage in joints is accompanied by structural and compositional changes in the tissue. Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage. NMR investigations of cartilage generally address only the relaxation properties of water. In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA. Two signal components were identified in all measurements, characterized by very different T2 which suggests liquid-like and solid-like dynamics. These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water. The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
format	article
author	Andrea Crețu Carlos Mattea Siegfried Stapf
author_facet	Andrea Crețu Carlos Mattea Siegfried Stapf
author_sort	Andrea Crețu
title	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
title_short	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
title_full	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
title_fullStr	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
title_full_unstemmed	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
title_sort	low-field and variable-field nmr relaxation studies of h2o and d2o molecular dynamics in articular cartilage.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/818ec6e728c34bd2bfa9056f55f7587e
work_keys_str_mv	AT andreacretu lowfieldandvariablefieldnmrrelaxationstudiesofh2oandd2omoleculardynamicsinarticularcartilage AT carlosmattea lowfieldandvariablefieldnmrrelaxationstudiesofh2oandd2omoleculardynamicsinarticularcartilage AT siegfriedstapf lowfieldandvariablefieldnmrrelaxationstudiesofh2oandd2omoleculardynamicsinarticularcartilage
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Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.

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