Physicochemical and Functional Characterization of Newly Designed Biopolymeric-Based Encapsulates with Probiotic Culture and Charantin

The identification of novel sources of synbiotic agents with desirable functionality is an emerging concept. In the present study, novel encapsulates containing probiotic <i>L. acidophilus</i> LA-05<sup>®</sup> (LA) and Charantin (CT) were produced by freeze-drying technique...

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Autores principales:	Awa Fanny Massounga Bora, Xiaodong Li, Lu Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	encapsulation synbiotic <i>Lactobacillus acidophilus</i> charantin whey protein isolate maltodextrin Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/8190ef8cf4344442819d4330f87fb3f4
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spelling	oai:doaj.org-article:8190ef8cf4344442819d4330f87fb3f42021-11-25T17:34:05ZPhysicochemical and Functional Characterization of Newly Designed Biopolymeric-Based Encapsulates with Probiotic Culture and Charantin10.3390/foods101126772304-8158https://doaj.org/article/8190ef8cf4344442819d4330f87fb3f42021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2677https://doaj.org/toc/2304-8158The identification of novel sources of synbiotic agents with desirable functionality is an emerging concept. In the present study, novel encapsulates containing probiotic <i>L. acidophilus</i> LA-05<sup>®</sup> (LA) and Charantin (CT) were produced by freeze-drying technique using pure Whey Protein Isolate (WPI), pure Maltodextrin (MD), and their combination (WPI + MD) in 1:1 core ratio, respectively. The obtained microparticles, namely WPI + LA + CT, MD + LA + CT, and WPI + MD + LA + CT were tested for their physicochemical properties. Among all formulations, combined carriers (WPI + MD) exhibited the highest encapsulation yields for LA (98%) and CT (75%). Microparticles showed a mean d (4, 3) ranging from 50.393 ± 1.26 to 68.412 ± 3.22 μm. The Scanning Electron Microscopy revealed uniformly amorphous and glass-like structures, with a noticeably reduced porosity when materials were combined. In addition, Fourier Transform Infrared spectroscopy highlighted the formation of strong hydrogen bonds supporting the interactions between the carrier materials (WPI and MD) and CT. In addition, the thermal stability of the combined WPI + MD was superior to that of pure WPI and pure MD, as depicted by the Thermogravimetric and Differential Scanning Calorimetry analysis. More interestingly, co-encapsulation with CT enhanced LA viability (8.91 ± 0.3 log CFU/g) and Cells Surface Hydrophobicity (82%) in vitro, in a prebiotic-like manner. Correspondingly, CT content was heightened when co-encapsulated with LA. Besides, WPI + MD + LA + CT microparticles exhibited higher antioxidant activity (79%), α-amylase inhibitory activity (83%), and lipase inhibitory activity (68%) than single carrier ones. Furthermore, LA viable count (7.95 ± 0.1 log CFU/g) and CT content (78%) were the highest in the blended carrier materials after 30 days of storage at 4 °C. Synbiotic microparticle WPI + MD + LA + CT represents an effective and promising approach for the co-delivery of probiotic culture and bioactive compounds in the digestive tract, with enhanced functionality and storage properties.Awa Fanny Massounga BoraXiaodong LiLu LiuMDPI AGarticleencapsulationsynbiotic<i>Lactobacillus acidophilus</i>charantinwhey protein isolatemaltodextrinChemical technologyTP1-1185ENFoods, Vol 10, Iss 2677, p 2677 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	encapsulation synbiotic <i>Lactobacillus acidophilus</i> charantin whey protein isolate maltodextrin Chemical technology TP1-1185
spellingShingle	encapsulation synbiotic <i>Lactobacillus acidophilus</i> charantin whey protein isolate maltodextrin Chemical technology TP1-1185 Awa Fanny Massounga Bora Xiaodong Li Lu Liu Physicochemical and Functional Characterization of Newly Designed Biopolymeric-Based Encapsulates with Probiotic Culture and Charantin
description	The identification of novel sources of synbiotic agents with desirable functionality is an emerging concept. In the present study, novel encapsulates containing probiotic <i>L. acidophilus</i> LA-05<sup>®</sup> (LA) and Charantin (CT) were produced by freeze-drying technique using pure Whey Protein Isolate (WPI), pure Maltodextrin (MD), and their combination (WPI + MD) in 1:1 core ratio, respectively. The obtained microparticles, namely WPI + LA + CT, MD + LA + CT, and WPI + MD + LA + CT were tested for their physicochemical properties. Among all formulations, combined carriers (WPI + MD) exhibited the highest encapsulation yields for LA (98%) and CT (75%). Microparticles showed a mean d (4, 3) ranging from 50.393 ± 1.26 to 68.412 ± 3.22 μm. The Scanning Electron Microscopy revealed uniformly amorphous and glass-like structures, with a noticeably reduced porosity when materials were combined. In addition, Fourier Transform Infrared spectroscopy highlighted the formation of strong hydrogen bonds supporting the interactions between the carrier materials (WPI and MD) and CT. In addition, the thermal stability of the combined WPI + MD was superior to that of pure WPI and pure MD, as depicted by the Thermogravimetric and Differential Scanning Calorimetry analysis. More interestingly, co-encapsulation with CT enhanced LA viability (8.91 ± 0.3 log CFU/g) and Cells Surface Hydrophobicity (82%) in vitro, in a prebiotic-like manner. Correspondingly, CT content was heightened when co-encapsulated with LA. Besides, WPI + MD + LA + CT microparticles exhibited higher antioxidant activity (79%), α-amylase inhibitory activity (83%), and lipase inhibitory activity (68%) than single carrier ones. Furthermore, LA viable count (7.95 ± 0.1 log CFU/g) and CT content (78%) were the highest in the blended carrier materials after 30 days of storage at 4 °C. Synbiotic microparticle WPI + MD + LA + CT represents an effective and promising approach for the co-delivery of probiotic culture and bioactive compounds in the digestive tract, with enhanced functionality and storage properties.
format	article
author	Awa Fanny Massounga Bora Xiaodong Li Lu Liu
author_facet	Awa Fanny Massounga Bora Xiaodong Li Lu Liu
author_sort	Awa Fanny Massounga Bora
title	Physicochemical and Functional Characterization of Newly Designed Biopolymeric-Based Encapsulates with Probiotic Culture and Charantin
title_short	Physicochemical and Functional Characterization of Newly Designed Biopolymeric-Based Encapsulates with Probiotic Culture and Charantin
title_full	Physicochemical and Functional Characterization of Newly Designed Biopolymeric-Based Encapsulates with Probiotic Culture and Charantin
title_fullStr	Physicochemical and Functional Characterization of Newly Designed Biopolymeric-Based Encapsulates with Probiotic Culture and Charantin
title_full_unstemmed	Physicochemical and Functional Characterization of Newly Designed Biopolymeric-Based Encapsulates with Probiotic Culture and Charantin
title_sort	physicochemical and functional characterization of newly designed biopolymeric-based encapsulates with probiotic culture and charantin
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8190ef8cf4344442819d4330f87fb3f4
work_keys_str_mv	AT awafannymassoungabora physicochemicalandfunctionalcharacterizationofnewlydesignedbiopolymericbasedencapsulateswithprobioticcultureandcharantin AT xiaodongli physicochemicalandfunctionalcharacterizationofnewlydesignedbiopolymericbasedencapsulateswithprobioticcultureandcharantin AT luliu physicochemicalandfunctionalcharacterizationofnewlydesignedbiopolymericbasedencapsulateswithprobioticcultureandcharantin
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Physicochemical and Functional Characterization of Newly Designed Biopolymeric-Based Encapsulates with Probiotic Culture and Charantin

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