Oleate but not stearate induces the regulatory phenotype of myeloid suppressor cells

Abstract Tumor infiltrating myeloid cells play contradictory roles in the tumor development. Dendritic cells and classical activated macrophages support anti-tumor immune activity via antigen presentation and induction of pro-inflammatory immune responses. Myeloid suppressor cells (MSCs), for instan...

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Autores principales:	Hao Wu, Carl Weidinger, Franziska Schmidt, Jacqueline Keye, Marie Friedrich, Cansu Yerinde, Gerald Willimsky, Zhihai Qin, Britta Siegmund, Rainer Glauben
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8191c67f68f7481b88466fe86aaca7ae
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spelling	oai:doaj.org-article:8191c67f68f7481b88466fe86aaca7ae2021-12-02T15:05:17ZOleate but not stearate induces the regulatory phenotype of myeloid suppressor cells10.1038/s41598-017-07685-92045-2322https://doaj.org/article/8191c67f68f7481b88466fe86aaca7ae2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07685-9https://doaj.org/toc/2045-2322Abstract Tumor infiltrating myeloid cells play contradictory roles in the tumor development. Dendritic cells and classical activated macrophages support anti-tumor immune activity via antigen presentation and induction of pro-inflammatory immune responses. Myeloid suppressor cells (MSCs), for instance myeloid derived suppressor cells (MDSCs) or tumor associated macrophages play a critical role in tumor growth. Here, treatment with sodium oleate, an unsaturated fatty acid, induced a regulatory phenotype in the myeloid suppressor cell line MSC-2 and resulted in an increased suppression of activated T cells, paralleled by increased intracellular lipid droplets formation. Furthermore, sodium oleate potentiated nitric oxide (NO) production in MSC-2, thereby increasing their suppressive capacity. In primary polarized bone marrow cells, sodium oleate (C18:1) and linoleate (C18:2), but not stearate (C18:0) were identified as potent FFA to induce a regulatory phenotype. This effect was abrogated in MSC-2 as well as primary cells by specific inhibition of droplets formation while the inhibition of de novo FFA synthesis proved ineffective, suggesting a critical role for exogenous FFA in the functional induction of MSCs. Taken together our data introduce a new unsaturated fatty acid-dependent pathway shaping the functional phenotype of MSCs, facilitating the tumor escape from the immune system.Hao WuCarl WeidingerFranziska SchmidtJacqueline KeyeMarie FriedrichCansu YerindeGerald WillimskyZhihai QinBritta SiegmundRainer GlaubenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hao Wu Carl Weidinger Franziska Schmidt Jacqueline Keye Marie Friedrich Cansu Yerinde Gerald Willimsky Zhihai Qin Britta Siegmund Rainer Glauben Oleate but not stearate induces the regulatory phenotype of myeloid suppressor cells
description	Abstract Tumor infiltrating myeloid cells play contradictory roles in the tumor development. Dendritic cells and classical activated macrophages support anti-tumor immune activity via antigen presentation and induction of pro-inflammatory immune responses. Myeloid suppressor cells (MSCs), for instance myeloid derived suppressor cells (MDSCs) or tumor associated macrophages play a critical role in tumor growth. Here, treatment with sodium oleate, an unsaturated fatty acid, induced a regulatory phenotype in the myeloid suppressor cell line MSC-2 and resulted in an increased suppression of activated T cells, paralleled by increased intracellular lipid droplets formation. Furthermore, sodium oleate potentiated nitric oxide (NO) production in MSC-2, thereby increasing their suppressive capacity. In primary polarized bone marrow cells, sodium oleate (C18:1) and linoleate (C18:2), but not stearate (C18:0) were identified as potent FFA to induce a regulatory phenotype. This effect was abrogated in MSC-2 as well as primary cells by specific inhibition of droplets formation while the inhibition of de novo FFA synthesis proved ineffective, suggesting a critical role for exogenous FFA in the functional induction of MSCs. Taken together our data introduce a new unsaturated fatty acid-dependent pathway shaping the functional phenotype of MSCs, facilitating the tumor escape from the immune system.
format	article
author	Hao Wu Carl Weidinger Franziska Schmidt Jacqueline Keye Marie Friedrich Cansu Yerinde Gerald Willimsky Zhihai Qin Britta Siegmund Rainer Glauben
author_facet	Hao Wu Carl Weidinger Franziska Schmidt Jacqueline Keye Marie Friedrich Cansu Yerinde Gerald Willimsky Zhihai Qin Britta Siegmund Rainer Glauben
author_sort	Hao Wu
title	Oleate but not stearate induces the regulatory phenotype of myeloid suppressor cells
title_short	Oleate but not stearate induces the regulatory phenotype of myeloid suppressor cells
title_full	Oleate but not stearate induces the regulatory phenotype of myeloid suppressor cells
title_fullStr	Oleate but not stearate induces the regulatory phenotype of myeloid suppressor cells
title_full_unstemmed	Oleate but not stearate induces the regulatory phenotype of myeloid suppressor cells
title_sort	oleate but not stearate induces the regulatory phenotype of myeloid suppressor cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8191c67f68f7481b88466fe86aaca7ae
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Oleate but not stearate induces the regulatory phenotype of myeloid suppressor cells

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