Knockdown of two Cadherin genes confers resistance to Cry2A and Cry1C in Chilo suppressalis

Abstract Bacillus thuringiensis (Bt) Cry toxins play an important role in the management of insect pests. Resistance to Bt toxins has been reported in many pest insects but the mechanism responsible for this resistance in rice crop pests remains largely unknown. Cadherin is one of several Bt toxin r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zan Zhang, Xiaolu Teng, Weihua Ma, Fei Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8198e2514abf42ffaf0ed798c1c47699
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8198e2514abf42ffaf0ed798c1c47699
record_format	dspace
spelling	oai:doaj.org-article:8198e2514abf42ffaf0ed798c1c476992021-12-02T12:32:05ZKnockdown of two Cadherin genes confers resistance to Cry2A and Cry1C in Chilo suppressalis10.1038/s41598-017-05110-92045-2322https://doaj.org/article/8198e2514abf42ffaf0ed798c1c476992017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05110-9https://doaj.org/toc/2045-2322Abstract Bacillus thuringiensis (Bt) Cry toxins play an important role in the management of insect pests. Resistance to Bt toxins has been reported in many pest insects but the mechanism responsible for this resistance in rice crop pests remains largely unknown. Cadherin is one of several Bt toxin receptors. At present, only one cadherin gene, CsCAD1, has been documented in the striped rice stem borer, Chilo suppressalis. We amplified a nearly full-length transcript of another C. suppressalis cadherin gene, CsCAD2, and found that it has a different expression pattern to CsCAD1. CsCAD1 was highly expressed in fifth and sixth instar larvae, especially in the midgut, while the expression levels of CsCA2 were equably in each developmental stage. Newly hatched larvae were fed on rice smeared with synthesized siRNA to knockdown either CsCAD1 or CsCAD2, and then were fed transgenic rice expressing either the Cry2A or Cry1C toxins. The siRNA-treatment groups had lower mortality and higher survival rates than the control group, suggesting that reduced expression of CsCAD1 or CsCAD2 increased resistance to Cry2A and Cry1C. We conclude that CsCAD1 and CsCAD2 interact with Bt toxins in C. suppressalis and that this interaction could be the mechanism underlying Bt resistance in this insect.Zan ZhangXiaolu TengWeihua MaFei LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zan Zhang Xiaolu Teng Weihua Ma Fei Li Knockdown of two Cadherin genes confers resistance to Cry2A and Cry1C in Chilo suppressalis
description	Abstract Bacillus thuringiensis (Bt) Cry toxins play an important role in the management of insect pests. Resistance to Bt toxins has been reported in many pest insects but the mechanism responsible for this resistance in rice crop pests remains largely unknown. Cadherin is one of several Bt toxin receptors. At present, only one cadherin gene, CsCAD1, has been documented in the striped rice stem borer, Chilo suppressalis. We amplified a nearly full-length transcript of another C. suppressalis cadherin gene, CsCAD2, and found that it has a different expression pattern to CsCAD1. CsCAD1 was highly expressed in fifth and sixth instar larvae, especially in the midgut, while the expression levels of CsCA2 were equably in each developmental stage. Newly hatched larvae were fed on rice smeared with synthesized siRNA to knockdown either CsCAD1 or CsCAD2, and then were fed transgenic rice expressing either the Cry2A or Cry1C toxins. The siRNA-treatment groups had lower mortality and higher survival rates than the control group, suggesting that reduced expression of CsCAD1 or CsCAD2 increased resistance to Cry2A and Cry1C. We conclude that CsCAD1 and CsCAD2 interact with Bt toxins in C. suppressalis and that this interaction could be the mechanism underlying Bt resistance in this insect.
format	article
author	Zan Zhang Xiaolu Teng Weihua Ma Fei Li
author_facet	Zan Zhang Xiaolu Teng Weihua Ma Fei Li
author_sort	Zan Zhang
title	Knockdown of two Cadherin genes confers resistance to Cry2A and Cry1C in Chilo suppressalis
title_short	Knockdown of two Cadherin genes confers resistance to Cry2A and Cry1C in Chilo suppressalis
title_full	Knockdown of two Cadherin genes confers resistance to Cry2A and Cry1C in Chilo suppressalis
title_fullStr	Knockdown of two Cadherin genes confers resistance to Cry2A and Cry1C in Chilo suppressalis
title_full_unstemmed	Knockdown of two Cadherin genes confers resistance to Cry2A and Cry1C in Chilo suppressalis
title_sort	knockdown of two cadherin genes confers resistance to cry2a and cry1c in chilo suppressalis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8198e2514abf42ffaf0ed798c1c47699
work_keys_str_mv	AT zanzhang knockdownoftwocadheringenesconfersresistancetocry2aandcry1cinchilosuppressalis AT xiaoluteng knockdownoftwocadheringenesconfersresistancetocry2aandcry1cinchilosuppressalis AT weihuama knockdownoftwocadheringenesconfersresistancetocry2aandcry1cinchilosuppressalis AT feili knockdownoftwocadheringenesconfersresistancetocry2aandcry1cinchilosuppressalis
_version_	1718394168280088576

Knockdown of two Cadherin genes confers resistance to Cry2A and Cry1C in Chilo suppressalis

Ejemplares similares