MicroRNA22-5p targets ten-eleven translocation and regulates estrogen receptor 2 expression in infertile women with minimal/mild endometriosis during implantation window.

Based on microRNA (miR) microarray analysis, we previously found that miR22-5p expression is decreased in the mid-luteal endometrium of women with minimal/mild endometriosis. Bioinformatics analysis predicted that miR22-5p targets ten-eleven translocation (TET2) 3'-untranslated region. This stu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Li Xiao, Tianjiao Pei, Wei Huang, Min Zhou, Jing Fu, Jing Tan, Tingting Liu, Yong Song, Shiyuan Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/81993f752c3543bb80c47c30ce7e64f9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:81993f752c3543bb80c47c30ce7e64f9
record_format	dspace
spelling	oai:doaj.org-article:81993f752c3543bb80c47c30ce7e64f92021-11-25T05:54:28ZMicroRNA22-5p targets ten-eleven translocation and regulates estrogen receptor 2 expression in infertile women with minimal/mild endometriosis during implantation window.1932-620310.1371/journal.pone.0234086https://doaj.org/article/81993f752c3543bb80c47c30ce7e64f92020-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0234086https://doaj.org/toc/1932-6203Based on microRNA (miR) microarray analysis, we previously found that miR22-5p expression is decreased in the mid-luteal endometrium of women with minimal/mild endometriosis. Bioinformatics analysis predicted that miR22-5p targets ten-eleven translocation (TET2) 3'-untranslated region. This study aimed to determine the regulation and roles of miR22-5p in the pathogenesis of minimal/mild endometriosis-associated infertility. MiR22-5p and TET2 expression in the mid-luteal endometrium from women with or without minimal/mild endometriosis was analyzed. After transfection with miR22-5p mimics or inhibitor, TET2 expression was analyzed by quantitative reverse transcription (RT-q) PCR, western blotting and immunohistochemistry. 5-Hydroxymethylcytosine was determined by immunofluorescence and dot blotting. Expression and promoter methylation of estrogen receptor 2 (ESR2) was measured by RT-qPCR and western blotting, and by bisulfite sequencing, respectively. We first established that miR22-5p expression decreased and TET2 expression increased in minimal/mild endometriosis during implantation window. TET2 was found to be a direct target of miR22-5p. MiR22-5p regulated the expression of ESR2, but did not directly affect methylation of its promoter region (-197/+359). Our results suggest that an imbalance in miR22-5p expression in the mid-luteal endometrium may be involved in minimal/mild endometriosis-associated infertility.Li XiaoTianjiao PeiWei HuangMin ZhouJing FuJing TanTingting LiuYong SongShiyuan YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 15, Iss 7, p e0234086 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Li Xiao Tianjiao Pei Wei Huang Min Zhou Jing Fu Jing Tan Tingting Liu Yong Song Shiyuan Yang MicroRNA22-5p targets ten-eleven translocation and regulates estrogen receptor 2 expression in infertile women with minimal/mild endometriosis during implantation window.
description	Based on microRNA (miR) microarray analysis, we previously found that miR22-5p expression is decreased in the mid-luteal endometrium of women with minimal/mild endometriosis. Bioinformatics analysis predicted that miR22-5p targets ten-eleven translocation (TET2) 3'-untranslated region. This study aimed to determine the regulation and roles of miR22-5p in the pathogenesis of minimal/mild endometriosis-associated infertility. MiR22-5p and TET2 expression in the mid-luteal endometrium from women with or without minimal/mild endometriosis was analyzed. After transfection with miR22-5p mimics or inhibitor, TET2 expression was analyzed by quantitative reverse transcription (RT-q) PCR, western blotting and immunohistochemistry. 5-Hydroxymethylcytosine was determined by immunofluorescence and dot blotting. Expression and promoter methylation of estrogen receptor 2 (ESR2) was measured by RT-qPCR and western blotting, and by bisulfite sequencing, respectively. We first established that miR22-5p expression decreased and TET2 expression increased in minimal/mild endometriosis during implantation window. TET2 was found to be a direct target of miR22-5p. MiR22-5p regulated the expression of ESR2, but did not directly affect methylation of its promoter region (-197/+359). Our results suggest that an imbalance in miR22-5p expression in the mid-luteal endometrium may be involved in minimal/mild endometriosis-associated infertility.
format	article
author	Li Xiao Tianjiao Pei Wei Huang Min Zhou Jing Fu Jing Tan Tingting Liu Yong Song Shiyuan Yang
author_facet	Li Xiao Tianjiao Pei Wei Huang Min Zhou Jing Fu Jing Tan Tingting Liu Yong Song Shiyuan Yang
author_sort	Li Xiao
title	MicroRNA22-5p targets ten-eleven translocation and regulates estrogen receptor 2 expression in infertile women with minimal/mild endometriosis during implantation window.
title_short	MicroRNA22-5p targets ten-eleven translocation and regulates estrogen receptor 2 expression in infertile women with minimal/mild endometriosis during implantation window.
title_full	MicroRNA22-5p targets ten-eleven translocation and regulates estrogen receptor 2 expression in infertile women with minimal/mild endometriosis during implantation window.
title_fullStr	MicroRNA22-5p targets ten-eleven translocation and regulates estrogen receptor 2 expression in infertile women with minimal/mild endometriosis during implantation window.
title_full_unstemmed	MicroRNA22-5p targets ten-eleven translocation and regulates estrogen receptor 2 expression in infertile women with minimal/mild endometriosis during implantation window.
title_sort	microrna22-5p targets ten-eleven translocation and regulates estrogen receptor 2 expression in infertile women with minimal/mild endometriosis during implantation window.
publisher	Public Library of Science (PLoS)
publishDate	2020
url	https://doaj.org/article/81993f752c3543bb80c47c30ce7e64f9
work_keys_str_mv	AT lixiao microrna225ptargetsteneleventranslocationandregulatesestrogenreceptor2expressionininfertilewomenwithminimalmildendometriosisduringimplantationwindow AT tianjiaopei microrna225ptargetsteneleventranslocationandregulatesestrogenreceptor2expressionininfertilewomenwithminimalmildendometriosisduringimplantationwindow AT weihuang microrna225ptargetsteneleventranslocationandregulatesestrogenreceptor2expressionininfertilewomenwithminimalmildendometriosisduringimplantationwindow AT minzhou microrna225ptargetsteneleventranslocationandregulatesestrogenreceptor2expressionininfertilewomenwithminimalmildendometriosisduringimplantationwindow AT jingfu microrna225ptargetsteneleventranslocationandregulatesestrogenreceptor2expressionininfertilewomenwithminimalmildendometriosisduringimplantationwindow AT jingtan microrna225ptargetsteneleventranslocationandregulatesestrogenreceptor2expressionininfertilewomenwithminimalmildendometriosisduringimplantationwindow AT tingtingliu microrna225ptargetsteneleventranslocationandregulatesestrogenreceptor2expressionininfertilewomenwithminimalmildendometriosisduringimplantationwindow AT yongsong microrna225ptargetsteneleventranslocationandregulatesestrogenreceptor2expressionininfertilewomenwithminimalmildendometriosisduringimplantationwindow AT shiyuanyang microrna225ptargetsteneleventranslocationandregulatesestrogenreceptor2expressionininfertilewomenwithminimalmildendometriosisduringimplantationwindow
_version_	1718414411360632832

MicroRNA22-5p targets ten-eleven translocation and regulates estrogen receptor 2 expression in infertile women with minimal/mild endometriosis during implantation window.

Ejemplares similares