Modulation of Expression of PVY<sup>NTN</sup> RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars

Potato virus Y (PVY) belongs to the genus Potyvirus and is considered to be one of the most harmful and important plant pathogens. Its RNA-dependent RNA polymerase (RdRp) is known as nuclear inclusion protein b (NIb). The recent findings show that the genome of PVY replicates in the cytoplasm of the...

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Autores principales:	Edmund Kozieł, Przemysław Surowiecki, Agnieszka Przewodowska, Józef J. Bujarski, Katarzyna Otulak-Kozieł
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	plant–virus interaction hypersensitive response heat shock cognate 70 protein nuclear inclusion b protein Medicine R
Acceso en línea:	https://doaj.org/article/819e1a06921847ce8a3a02357298b3fd
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spelling	oai:doaj.org-article:819e1a06921847ce8a3a02357298b3fd2021-11-25T19:10:34ZModulation of Expression of PVY<sup>NTN</sup> RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars10.3390/vaccines91112542076-393Xhttps://doaj.org/article/819e1a06921847ce8a3a02357298b3fd2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1254https://doaj.org/toc/2076-393XPotato virus Y (PVY) belongs to the genus Potyvirus and is considered to be one of the most harmful and important plant pathogens. Its RNA-dependent RNA polymerase (RdRp) is known as nuclear inclusion protein b (NIb). The recent findings show that the genome of PVY replicates in the cytoplasm of the plant cell by binding the virus replication complex to the membranous structures of different organelles. In some potyviruses, NIb has been found to be localized in the nucleus and associated with the endoplasmic reticulum membranes. Moreover, NIb has been shown to interact with other host proteins that are particularly involved in promoting the virus infection cycle, such as the heat shock proteins (HSPs). HSP70 is the most conserved among the five major HSP families that are known to affect the plant–pathogen interactions. Some plant viruses can induce the production of HSP70 during the development of infection. To understand the molecular mechanisms underlying the interactive response to PVY<sup>NTN</sup> (necrotic tuber necrosis strain of PVY), the present study focused on <i>StHSC70-8</i> and PVY<sup>NTN</sup>-<i>NIb</i> gene expression via localization of HSC70 and NIb proteins during compatible (susceptible) and incompatible (hypersensitive) potato–PVY<sup>NTN</sup> interactions. Our results demonstrate that NIb and HSC70 are involved in the response to PVY<sup>NTN</sup> infections and probably cooperate at some stages of the virus infection cycle. Enhanced deposition of HSC70 proteins during the infection cycle was associated with the dynamic induction of PVY<sup>NTN</sup>-<i>NIb</i> gene expression and NIb localization during susceptible infections. In hypersensitive response (HR), a significant increase in HSC70 expression was observed up to 3 days post-inoculation (dpi) in the nucleus and chloroplasts. Thereafter, between 3 and 21 dpi, the deposition of NIb decreased, which can be attributed to a reduction in the levels of both virus accumulation and PVY<sup>NTN</sup>-<i>NIb</i> gene expression. Therefore, we postulate that increase in the expression of both <i>StHSC70-8</i> and PVY<sup>NTN</sup>-<i>NIb</i> induces the PVY infection during susceptible infections. In contrast, during HRs, HSC70 cooperates with PVY<sup>NTN</sup> only at the early stages of interaction and mediates the defense response signaling pathway at the later stages of infection.Edmund KoziełPrzemysław SurowieckiAgnieszka PrzewodowskaJózef J. BujarskiKatarzyna Otulak-KoziełMDPI AGarticleplant–virus interactionhypersensitive responseheat shock cognate 70 proteinnuclear inclusion b proteinMedicineRENVaccines, Vol 9, Iss 1254, p 1254 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plant–virus interaction hypersensitive response heat shock cognate 70 protein nuclear inclusion b protein Medicine R
spellingShingle	plant–virus interaction hypersensitive response heat shock cognate 70 protein nuclear inclusion b protein Medicine R Edmund Kozieł Przemysław Surowiecki Agnieszka Przewodowska Józef J. Bujarski Katarzyna Otulak-Kozieł Modulation of Expression of PVY<sup>NTN</sup> RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars
description	Potato virus Y (PVY) belongs to the genus Potyvirus and is considered to be one of the most harmful and important plant pathogens. Its RNA-dependent RNA polymerase (RdRp) is known as nuclear inclusion protein b (NIb). The recent findings show that the genome of PVY replicates in the cytoplasm of the plant cell by binding the virus replication complex to the membranous structures of different organelles. In some potyviruses, NIb has been found to be localized in the nucleus and associated with the endoplasmic reticulum membranes. Moreover, NIb has been shown to interact with other host proteins that are particularly involved in promoting the virus infection cycle, such as the heat shock proteins (HSPs). HSP70 is the most conserved among the five major HSP families that are known to affect the plant–pathogen interactions. Some plant viruses can induce the production of HSP70 during the development of infection. To understand the molecular mechanisms underlying the interactive response to PVY<sup>NTN</sup> (necrotic tuber necrosis strain of PVY), the present study focused on <i>StHSC70-8</i> and PVY<sup>NTN</sup>-<i>NIb</i> gene expression via localization of HSC70 and NIb proteins during compatible (susceptible) and incompatible (hypersensitive) potato–PVY<sup>NTN</sup> interactions. Our results demonstrate that NIb and HSC70 are involved in the response to PVY<sup>NTN</sup> infections and probably cooperate at some stages of the virus infection cycle. Enhanced deposition of HSC70 proteins during the infection cycle was associated with the dynamic induction of PVY<sup>NTN</sup>-<i>NIb</i> gene expression and NIb localization during susceptible infections. In hypersensitive response (HR), a significant increase in HSC70 expression was observed up to 3 days post-inoculation (dpi) in the nucleus and chloroplasts. Thereafter, between 3 and 21 dpi, the deposition of NIb decreased, which can be attributed to a reduction in the levels of both virus accumulation and PVY<sup>NTN</sup>-<i>NIb</i> gene expression. Therefore, we postulate that increase in the expression of both <i>StHSC70-8</i> and PVY<sup>NTN</sup>-<i>NIb</i> induces the PVY infection during susceptible infections. In contrast, during HRs, HSC70 cooperates with PVY<sup>NTN</sup> only at the early stages of interaction and mediates the defense response signaling pathway at the later stages of infection.
format	article
author	Edmund Kozieł Przemysław Surowiecki Agnieszka Przewodowska Józef J. Bujarski Katarzyna Otulak-Kozieł
author_facet	Edmund Kozieł Przemysław Surowiecki Agnieszka Przewodowska Józef J. Bujarski Katarzyna Otulak-Kozieł
author_sort	Edmund Kozieł
title	Modulation of Expression of PVY<sup>NTN</sup> RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars
title_short	Modulation of Expression of PVY<sup>NTN</sup> RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars
title_full	Modulation of Expression of PVY<sup>NTN</sup> RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars
title_fullStr	Modulation of Expression of PVY<sup>NTN</sup> RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars
title_full_unstemmed	Modulation of Expression of PVY<sup>NTN</sup> RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars
title_sort	modulation of expression of pvy<sup>ntn</sup> rna-dependent rna polymerase (nib) and heat shock cognate host protein hsc70 in susceptible and hypersensitive potato cultivars
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/819e1a06921847ce8a3a02357298b3fd
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Modulation of Expression of PVY<sup>NTN</sup> RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars

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