Transcriptomics and metabolomics reveal the adaption of Akkermansia muciniphila to high mucin by regulating energy homeostasis

Abstract In gut, Akkermansia muciniphila (A. muciniphila) probably exerts its probiotic activities by the positive modulation of mucus thickness and gut barrier integrity. However, the potential mechanisms between A. muciniphila and mucin balance have not been fully elucidated. In this study, we cul...

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Autores principales:	Xinyue Liu, Fan Zhao, Hui Liu, Yunting Xie, Di Zhao, Chunbao Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/81a4981d23f244f89fdbeb033c2eba07
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spelling	oai:doaj.org-article:81a4981d23f244f89fdbeb033c2eba072021-12-02T17:39:31ZTranscriptomics and metabolomics reveal the adaption of Akkermansia muciniphila to high mucin by regulating energy homeostasis10.1038/s41598-021-88397-z2045-2322https://doaj.org/article/81a4981d23f244f89fdbeb033c2eba072021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88397-zhttps://doaj.org/toc/2045-2322Abstract In gut, Akkermansia muciniphila (A. muciniphila) probably exerts its probiotic activities by the positive modulation of mucus thickness and gut barrier integrity. However, the potential mechanisms between A. muciniphila and mucin balance have not been fully elucidated. In this study, we cultured the bacterium in a BHI medium containing 0% to 0.5% mucin, and transcriptome and gas chromatography mass spectrometry (GC–MS) analyses were performed. We found that 0.5% (m/v) mucin in a BHI medium induced 1191 microbial genes to be differentially expressed, and 49 metabolites to be changed. The metabolites of sorbose, mannose, 2,7-anhydro-β-sedoheptulose, fructose, phenylalanine, threonine, lysine, ornithine, asparagine, alanine and glutamic acid were decreased by 0.5% mucin, while the metabolites of leucine, valine and N-acetylneuraminic acid were increased. The association analysis between transcriptome and metabolome revealed that A. muciniphila gave strong responses to energy metabolism, amino sugar and nucleotide sugar metabolism, and galactose metabolism pathways to adapt to high mucin in the medium. This finding showed that only when mucin reached a certain concentration in a BHI medium, A. muciniphila could respond to the culture environment significantly at the level of genes and metabolites, and changed its metabolic characteristics by altering the effect on carbohydrates and amino acids.Xinyue LiuFan ZhaoHui LiuYunting XieDi ZhaoChunbao LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xinyue Liu Fan Zhao Hui Liu Yunting Xie Di Zhao Chunbao Li Transcriptomics and metabolomics reveal the adaption of Akkermansia muciniphila to high mucin by regulating energy homeostasis
description	Abstract In gut, Akkermansia muciniphila (A. muciniphila) probably exerts its probiotic activities by the positive modulation of mucus thickness and gut barrier integrity. However, the potential mechanisms between A. muciniphila and mucin balance have not been fully elucidated. In this study, we cultured the bacterium in a BHI medium containing 0% to 0.5% mucin, and transcriptome and gas chromatography mass spectrometry (GC–MS) analyses were performed. We found that 0.5% (m/v) mucin in a BHI medium induced 1191 microbial genes to be differentially expressed, and 49 metabolites to be changed. The metabolites of sorbose, mannose, 2,7-anhydro-β-sedoheptulose, fructose, phenylalanine, threonine, lysine, ornithine, asparagine, alanine and glutamic acid were decreased by 0.5% mucin, while the metabolites of leucine, valine and N-acetylneuraminic acid were increased. The association analysis between transcriptome and metabolome revealed that A. muciniphila gave strong responses to energy metabolism, amino sugar and nucleotide sugar metabolism, and galactose metabolism pathways to adapt to high mucin in the medium. This finding showed that only when mucin reached a certain concentration in a BHI medium, A. muciniphila could respond to the culture environment significantly at the level of genes and metabolites, and changed its metabolic characteristics by altering the effect on carbohydrates and amino acids.
format	article
author	Xinyue Liu Fan Zhao Hui Liu Yunting Xie Di Zhao Chunbao Li
author_facet	Xinyue Liu Fan Zhao Hui Liu Yunting Xie Di Zhao Chunbao Li
author_sort	Xinyue Liu
title	Transcriptomics and metabolomics reveal the adaption of Akkermansia muciniphila to high mucin by regulating energy homeostasis
title_short	Transcriptomics and metabolomics reveal the adaption of Akkermansia muciniphila to high mucin by regulating energy homeostasis
title_full	Transcriptomics and metabolomics reveal the adaption of Akkermansia muciniphila to high mucin by regulating energy homeostasis
title_fullStr	Transcriptomics and metabolomics reveal the adaption of Akkermansia muciniphila to high mucin by regulating energy homeostasis
title_full_unstemmed	Transcriptomics and metabolomics reveal the adaption of Akkermansia muciniphila to high mucin by regulating energy homeostasis
title_sort	transcriptomics and metabolomics reveal the adaption of akkermansia muciniphila to high mucin by regulating energy homeostasis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/81a4981d23f244f89fdbeb033c2eba07
work_keys_str_mv	AT xinyueliu transcriptomicsandmetabolomicsrevealtheadaptionofakkermansiamuciniphilatohighmucinbyregulatingenergyhomeostasis AT fanzhao transcriptomicsandmetabolomicsrevealtheadaptionofakkermansiamuciniphilatohighmucinbyregulatingenergyhomeostasis AT huiliu transcriptomicsandmetabolomicsrevealtheadaptionofakkermansiamuciniphilatohighmucinbyregulatingenergyhomeostasis AT yuntingxie transcriptomicsandmetabolomicsrevealtheadaptionofakkermansiamuciniphilatohighmucinbyregulatingenergyhomeostasis AT dizhao transcriptomicsandmetabolomicsrevealtheadaptionofakkermansiamuciniphilatohighmucinbyregulatingenergyhomeostasis AT chunbaoli transcriptomicsandmetabolomicsrevealtheadaptionofakkermansiamuciniphilatohighmucinbyregulatingenergyhomeostasis
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Transcriptomics and metabolomics reveal the adaption of Akkermansia muciniphila to high mucin by regulating energy homeostasis

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