Evaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury

Venu Kesireddy1,2 1Wake Forest Institute for Regenerative Medicine, Wake Forest University Baptist Medical Center, Winston Salem, NC, USA; 2Center for Craniofacial Research, School of Dentistry, University of Texas Health Science Center at Houston, Houston, TX, USA Abstract: Volumetric muscle loss...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autor principal: Kesireddy V
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://doaj.org/article/81a5ae55bff349f59a774d2d4b6c4466
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:81a5ae55bff349f59a774d2d4b6c4466
record_format dspace
spelling oai:doaj.org-article:81a5ae55bff349f59a774d2d4b6c44662021-12-02T00:39:10ZEvaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury1178-2013https://doaj.org/article/81a5ae55bff349f59a774d2d4b6c44662016-04-01T00:00:00Zhttps://www.dovepress.com/evaluation-of-adipose-derived-stem-cells-for-tissue-engineered-muscle--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Venu Kesireddy1,2 1Wake Forest Institute for Regenerative Medicine, Wake Forest University Baptist Medical Center, Winston Salem, NC, USA; 2Center for Craniofacial Research, School of Dentistry, University of Texas Health Science Center at Houston, Houston, TX, USA Abstract: Volumetric muscle loss (VML) can occur from congenital defects, muscle wasting diseases, civilian or military injuries, and as a result of surgical removal of muscle tissue (eg, cancer), all of which can lead to irrevocable functional and cosmetic defects. Current tissue engineering strategies to repair VML often employ muscle-derived progenitor cells (MDCs) as one component. However, there are some inherent limitations in their in vitro culture expansion. Thus, this study explores the potential of adipose-derived stem cells (ADSCs) as an alternative cell source to MDCs for tissue engineering of skeletal muscle. A reproducible VML injury model in murine latissimus dorsi muscle was used to evaluate tissue-engineered muscle repair (TEMR) constructs incorporating MDCs or ADSCs. Importantly, histological analysis revealed that ADSC-seeded constructs displayed regeneration potential that was comparable to those seeded with MDCs 2 months postrepair. Furthermore, morphological analysis of retrieved constructs demonstrated signs of neotissue formation, including cell fusion, fiber formation, and scaffold remodeling. Immunohistochemistry demonstrated positive staining for both structural and functional proteins. Positive staining for vascular structures indicated the potential for long-term neotissue survival and integration with existing musculature. Qualitative observation of lentivirus-Cherry-labeled donor cells by immunohistochemistry indicates that participation of ADSCs in new hybrid myofiber formation incorporating donor cells was relatively low, compared to donor MDCs. However, ADSCs appear to participate in vascularization. In summary, I have demonstrated that TEMR constructs generated with ADSCs displayed skeletal muscle regeneration potential comparable to TEMR–MDC constructs as previously reported. Keywords: skeletal muscle regeneration, muscle-derived progenitor cells, immunomodulation, paracrine signalingKesireddy VDove Medical PressarticleSkeletal muscle regenerationAdipose derived stem cellsmuscle derived progenitor cellsTissue engineered muscle repair constructvolumetric muscle lossMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1461-1473 (2016)
institution DOAJ
collection DOAJ
language EN
topic Skeletal muscle regeneration
Adipose derived stem cells
muscle derived progenitor cells
Tissue engineered muscle repair construct
volumetric muscle loss
Medicine (General)
R5-920
spellingShingle Skeletal muscle regeneration
Adipose derived stem cells
muscle derived progenitor cells
Tissue engineered muscle repair construct
volumetric muscle loss
Medicine (General)
R5-920
Kesireddy V
Evaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury
description Venu Kesireddy1,2 1Wake Forest Institute for Regenerative Medicine, Wake Forest University Baptist Medical Center, Winston Salem, NC, USA; 2Center for Craniofacial Research, School of Dentistry, University of Texas Health Science Center at Houston, Houston, TX, USA Abstract: Volumetric muscle loss (VML) can occur from congenital defects, muscle wasting diseases, civilian or military injuries, and as a result of surgical removal of muscle tissue (eg, cancer), all of which can lead to irrevocable functional and cosmetic defects. Current tissue engineering strategies to repair VML often employ muscle-derived progenitor cells (MDCs) as one component. However, there are some inherent limitations in their in vitro culture expansion. Thus, this study explores the potential of adipose-derived stem cells (ADSCs) as an alternative cell source to MDCs for tissue engineering of skeletal muscle. A reproducible VML injury model in murine latissimus dorsi muscle was used to evaluate tissue-engineered muscle repair (TEMR) constructs incorporating MDCs or ADSCs. Importantly, histological analysis revealed that ADSC-seeded constructs displayed regeneration potential that was comparable to those seeded with MDCs 2 months postrepair. Furthermore, morphological analysis of retrieved constructs demonstrated signs of neotissue formation, including cell fusion, fiber formation, and scaffold remodeling. Immunohistochemistry demonstrated positive staining for both structural and functional proteins. Positive staining for vascular structures indicated the potential for long-term neotissue survival and integration with existing musculature. Qualitative observation of lentivirus-Cherry-labeled donor cells by immunohistochemistry indicates that participation of ADSCs in new hybrid myofiber formation incorporating donor cells was relatively low, compared to donor MDCs. However, ADSCs appear to participate in vascularization. In summary, I have demonstrated that TEMR constructs generated with ADSCs displayed skeletal muscle regeneration potential comparable to TEMR–MDC constructs as previously reported. Keywords: skeletal muscle regeneration, muscle-derived progenitor cells, immunomodulation, paracrine signaling
format article
author Kesireddy V
author_facet Kesireddy V
author_sort Kesireddy V
title Evaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury
title_short Evaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury
title_full Evaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury
title_fullStr Evaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury
title_full_unstemmed Evaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury
title_sort evaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/81a5ae55bff349f59a774d2d4b6c4466
work_keys_str_mv AT kesireddyv evaluationofadiposederivedstemcellsfortissueengineeredmusclerepairconstructmediatedrepairofamurinemodelofvolumetricmusclelossinjury
_version_ 1718403565450428416