Basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold

Abstract Native bovine pericardium (BP) exhibits anisotropy of its surface ECM niches, with the serous surface (i.e., parietal pericardium) containing basement membrane components (e.g., Laminin, Col IV) and the fibrous surface (i.e., mediastinal side) being composed primarily of type I collagen (Co...

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Autores principales:	Qi Xing, Mojtaba Parvizi, Manuela Lopera Higuita, Leigh G. Griffiths
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/81a7d1368784497f92c30d9d23108e65
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spelling	oai:doaj.org-article:81a7d1368784497f92c30d9d23108e652021-12-02T13:33:45ZBasement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold10.1038/s41598-021-84161-52045-2322https://doaj.org/article/81a7d1368784497f92c30d9d23108e652021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84161-5https://doaj.org/toc/2045-2322Abstract Native bovine pericardium (BP) exhibits anisotropy of its surface ECM niches, with the serous surface (i.e., parietal pericardium) containing basement membrane components (e.g., Laminin, Col IV) and the fibrous surface (i.e., mediastinal side) being composed primarily of type I collagen (Col I). Native BP surface ECM niche anisotropy is preserved in antigen removed BP (AR-BP) extracellular matrix (ECM) scaffolds. By exploiting sideness (serous or fibrous surface) of AR-BP scaffolds, this study aims to determine the mechanism by which ECM niche influences human mesenchymal stem cells (hMSCs) migration. Human mesenchymal stem cells (hMSC) seeding on serous surface promoted more rapid cell migration than fibrous surface seeding. Gene analysis revealed that expression of integrin α3 and α11 were increased in cells cultured on serous surface compared to those on the fibrous side. Monoclonal antibody blockade of α3β1 (i.e., laminin binding) inhibited early (i.e. ≤ 6 h) hMSC migration following serous seeding, while having no effect on migration of cells on the fibrous side. Blockade of α3β1 resulted in decreased expression of integrin α3 by cells on serous surface. Monoclonal antibody blockade of α11β1 (i.e., Col IV binding) inhibited serous side migration at later time points (i.e., 6–24 h). These results confirmed the role of integrin α3β1 binding to laminin in mediating early rapid hMSCs migration and α11β1 binding to Col IV in mediating later hMSCs migration on the serous side of AR-BP, which has critical implications for rate of cellular monolayer formation and use of AR-BP as blood contacting material for clinical applications.Qi XingMojtaba ParviziManuela Lopera HiguitaLeigh G. GriffithsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qi Xing Mojtaba Parvizi Manuela Lopera Higuita Leigh G. Griffiths Basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold
description	Abstract Native bovine pericardium (BP) exhibits anisotropy of its surface ECM niches, with the serous surface (i.e., parietal pericardium) containing basement membrane components (e.g., Laminin, Col IV) and the fibrous surface (i.e., mediastinal side) being composed primarily of type I collagen (Col I). Native BP surface ECM niche anisotropy is preserved in antigen removed BP (AR-BP) extracellular matrix (ECM) scaffolds. By exploiting sideness (serous or fibrous surface) of AR-BP scaffolds, this study aims to determine the mechanism by which ECM niche influences human mesenchymal stem cells (hMSCs) migration. Human mesenchymal stem cells (hMSC) seeding on serous surface promoted more rapid cell migration than fibrous surface seeding. Gene analysis revealed that expression of integrin α3 and α11 were increased in cells cultured on serous surface compared to those on the fibrous side. Monoclonal antibody blockade of α3β1 (i.e., laminin binding) inhibited early (i.e. ≤ 6 h) hMSC migration following serous seeding, while having no effect on migration of cells on the fibrous side. Blockade of α3β1 resulted in decreased expression of integrin α3 by cells on serous surface. Monoclonal antibody blockade of α11β1 (i.e., Col IV binding) inhibited serous side migration at later time points (i.e., 6–24 h). These results confirmed the role of integrin α3β1 binding to laminin in mediating early rapid hMSCs migration and α11β1 binding to Col IV in mediating later hMSCs migration on the serous side of AR-BP, which has critical implications for rate of cellular monolayer formation and use of AR-BP as blood contacting material for clinical applications.
format	article
author	Qi Xing Mojtaba Parvizi Manuela Lopera Higuita Leigh G. Griffiths
author_facet	Qi Xing Mojtaba Parvizi Manuela Lopera Higuita Leigh G. Griffiths
author_sort	Qi Xing
title	Basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold
title_short	Basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold
title_full	Basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold
title_fullStr	Basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold
title_full_unstemmed	Basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold
title_sort	basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/81a7d1368784497f92c30d9d23108e65
work_keys_str_mv	AT qixing basementmembraneproteinsmodulatecellmigrationonbovinepericardiumextracellularmatrixscaffold AT mojtabaparvizi basementmembraneproteinsmodulatecellmigrationonbovinepericardiumextracellularmatrixscaffold AT manuelaloperahiguita basementmembraneproteinsmodulatecellmigrationonbovinepericardiumextracellularmatrixscaffold AT leighggriffiths basementmembraneproteinsmodulatecellmigrationonbovinepericardiumextracellularmatrixscaffold
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Basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold

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