Development and validation of a gas chromatography method for the determination of β-caryophyllene in clove extract and its application

Abstract The purpose of this study is to check the effectiveness of the analysis method that separates and quantifies β-caryophyllene among clove extracts and validate according to current ICH guidelines. The β-caryophyllene was active constituent of clove buds. The developed method gave a good dete...

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Autores principales:	Mi Hee Park, Chul Jin Kim, Jin Young Lee, In Seon Kim, Sung-Kyu Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/81a97a6b4d654ef58fb9553496f7113b
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spelling	oai:doaj.org-article:81a97a6b4d654ef58fb9553496f7113b2021-12-02T15:39:41ZDevelopment and validation of a gas chromatography method for the determination of β-caryophyllene in clove extract and its application10.1038/s41598-021-93306-52045-2322https://doaj.org/article/81a97a6b4d654ef58fb9553496f7113b2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93306-5https://doaj.org/toc/2045-2322Abstract The purpose of this study is to check the effectiveness of the analysis method that separates and quantifies β-caryophyllene among clove extracts and validate according to current ICH guidelines. The β-caryophyllene was active constituent of clove buds. The developed method gave a good detection response. In the specificity test, the standard solution was detected at about 17.32 min, and the test solution was detected at 17.32 min. The linearity of β-caryophyllen was confirmed, and at this time, the correlation coefficient (R2) of the calibration curve showed a high linearity of 0.999 or more in the concentration range. The levels of LOD and LOQ were 1.28 ug/mL and 3.89 ug/mL, respectively. The accuracy was confirmed to be 101.6–102.2% and RSD 0.95 ~ 1.31%. As a result of checking the repeatability and inter-tester reproducibility to confirm the precision, the RSD was found to be 1.34 ~ 2.69%. This validated GC method was successfully applied to a soft capsule containing clove extract and other materials for clinical trials. Therefore, this method can be used as an analytical tool for quality control of various samples, including clove extracts and their products of food and pharmaceutical uses.Mi Hee ParkChul Jin KimJin Young LeeIn Seon KimSung-Kyu KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mi Hee Park Chul Jin Kim Jin Young Lee In Seon Kim Sung-Kyu Kim Development and validation of a gas chromatography method for the determination of β-caryophyllene in clove extract and its application
description	Abstract The purpose of this study is to check the effectiveness of the analysis method that separates and quantifies β-caryophyllene among clove extracts and validate according to current ICH guidelines. The β-caryophyllene was active constituent of clove buds. The developed method gave a good detection response. In the specificity test, the standard solution was detected at about 17.32 min, and the test solution was detected at 17.32 min. The linearity of β-caryophyllen was confirmed, and at this time, the correlation coefficient (R2) of the calibration curve showed a high linearity of 0.999 or more in the concentration range. The levels of LOD and LOQ were 1.28 ug/mL and 3.89 ug/mL, respectively. The accuracy was confirmed to be 101.6–102.2% and RSD 0.95 ~ 1.31%. As a result of checking the repeatability and inter-tester reproducibility to confirm the precision, the RSD was found to be 1.34 ~ 2.69%. This validated GC method was successfully applied to a soft capsule containing clove extract and other materials for clinical trials. Therefore, this method can be used as an analytical tool for quality control of various samples, including clove extracts and their products of food and pharmaceutical uses.
format	article
author	Mi Hee Park Chul Jin Kim Jin Young Lee In Seon Kim Sung-Kyu Kim
author_facet	Mi Hee Park Chul Jin Kim Jin Young Lee In Seon Kim Sung-Kyu Kim
author_sort	Mi Hee Park
title	Development and validation of a gas chromatography method for the determination of β-caryophyllene in clove extract and its application
title_short	Development and validation of a gas chromatography method for the determination of β-caryophyllene in clove extract and its application
title_full	Development and validation of a gas chromatography method for the determination of β-caryophyllene in clove extract and its application
title_fullStr	Development and validation of a gas chromatography method for the determination of β-caryophyllene in clove extract and its application
title_full_unstemmed	Development and validation of a gas chromatography method for the determination of β-caryophyllene in clove extract and its application
title_sort	development and validation of a gas chromatography method for the determination of β-caryophyllene in clove extract and its application
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/81a97a6b4d654ef58fb9553496f7113b
work_keys_str_mv	AT miheepark developmentandvalidationofagaschromatographymethodforthedeterminationofbcaryophylleneincloveextractanditsapplication AT chuljinkim developmentandvalidationofagaschromatographymethodforthedeterminationofbcaryophylleneincloveextractanditsapplication AT jinyounglee developmentandvalidationofagaschromatographymethodforthedeterminationofbcaryophylleneincloveextractanditsapplication AT inseonkim developmentandvalidationofagaschromatographymethodforthedeterminationofbcaryophylleneincloveextractanditsapplication AT sungkyukim developmentandvalidationofagaschromatographymethodforthedeterminationofbcaryophylleneincloveextractanditsapplication
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Development and validation of a gas chromatography method for the determination of β-caryophyllene in clove extract and its application

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