The pan-genome of the animal pathogen Corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains.

Corynebacterium pseudotuberculosis is a facultative intracellular pathogen and the causative agent of several infectious and contagious chronic diseases, including caseous lymphadenitis, ulcerative lymphangitis, mastitis, and edematous skin disease, in a broad spectrum of hosts. In addition, Coryneb...

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Autores principales:	Siomar C Soares, Artur Silva, Eva Trost, Jochen Blom, Rommel Ramos, Adriana Carneiro, Amjad Ali, Anderson R Santos, Anne C Pinto, Carlos Diniz, Eudes G V Barbosa, Fernanda A Dorella, Flávia Aburjaile, Flávia S Rocha, Karina K F Nascimento, Luís C Guimarães, Sintia Almeida, Syed S Hassan, Syeda M Bakhtiar, Ulisses P Pereira, Vinicius A C Abreu, Maria P C Schneider, Anderson Miyoshi, Andreas Tauch, Vasco Azevedo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/81ae3ebdc6ff46e6a121005fed57fb82
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spelling	oai:doaj.org-article:81ae3ebdc6ff46e6a121005fed57fb822021-11-18T08:01:35ZThe pan-genome of the animal pathogen Corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains.1932-620310.1371/journal.pone.0053818https://doaj.org/article/81ae3ebdc6ff46e6a121005fed57fb822013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23342011/?tool=EBIhttps://doaj.org/toc/1932-6203Corynebacterium pseudotuberculosis is a facultative intracellular pathogen and the causative agent of several infectious and contagious chronic diseases, including caseous lymphadenitis, ulcerative lymphangitis, mastitis, and edematous skin disease, in a broad spectrum of hosts. In addition, Corynebacterium pseudotuberculosis infections pose a rising worldwide economic problem in ruminants. The complete genome sequences of 15 C. pseudotuberculosis strains isolated from different hosts and countries were comparatively analyzed using a pan-genomic strategy. Phylogenomic, pan-genomic, core genomic, and singleton analyses revealed close relationships among pathogenic corynebacteria, the clonal-like behavior of C. pseudotuberculosis and slow increases in the sizes of pan-genomes. According to extrapolations based on the pan-genomes, core genomes and singletons, the C. pseudotuberculosis biovar ovis shows a more clonal-like behavior than the C. pseudotuberculosis biovar equi. Most of the variable genes of the biovar ovis strains were acquired in a block through horizontal gene transfer and are highly conserved, whereas the biovar equi strains contain great variability, both intra- and inter-biovar, in the 16 detected pathogenicity islands (PAIs). With respect to the gene content of the PAIs, the most interesting finding is the high similarity of the pilus genes in the biovar ovis strains compared with the great variability of these genes in the biovar equi strains. Concluding, the polymerization of complete pilus structures in biovar ovis could be responsible for a remarkable ability of these strains to spread throughout host tissues and penetrate cells to live intracellularly, in contrast with the biovar equi, which rarely attacks visceral organs. Intracellularly, the biovar ovis strains are expected to have less contact with other organisms than the biovar equi strains, thereby explaining the significant clonal-like behavior of the biovar ovis strains.Siomar C SoaresArtur SilvaEva TrostJochen BlomRommel RamosAdriana CarneiroAmjad AliAnderson R SantosAnne C PintoCarlos DinizEudes G V BarbosaFernanda A DorellaFlávia AburjaileFlávia S RochaKarina K F NascimentoLuís C GuimarãesSintia AlmeidaSyed S HassanSyeda M BakhtiarUlisses P PereiraVinicius A C AbreuMaria P C SchneiderAnderson MiyoshiAndreas TauchVasco AzevedoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53818 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Siomar C Soares Artur Silva Eva Trost Jochen Blom Rommel Ramos Adriana Carneiro Amjad Ali Anderson R Santos Anne C Pinto Carlos Diniz Eudes G V Barbosa Fernanda A Dorella Flávia Aburjaile Flávia S Rocha Karina K F Nascimento Luís C Guimarães Sintia Almeida Syed S Hassan Syeda M Bakhtiar Ulisses P Pereira Vinicius A C Abreu Maria P C Schneider Anderson Miyoshi Andreas Tauch Vasco Azevedo The pan-genome of the animal pathogen Corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains.
description	Corynebacterium pseudotuberculosis is a facultative intracellular pathogen and the causative agent of several infectious and contagious chronic diseases, including caseous lymphadenitis, ulcerative lymphangitis, mastitis, and edematous skin disease, in a broad spectrum of hosts. In addition, Corynebacterium pseudotuberculosis infections pose a rising worldwide economic problem in ruminants. The complete genome sequences of 15 C. pseudotuberculosis strains isolated from different hosts and countries were comparatively analyzed using a pan-genomic strategy. Phylogenomic, pan-genomic, core genomic, and singleton analyses revealed close relationships among pathogenic corynebacteria, the clonal-like behavior of C. pseudotuberculosis and slow increases in the sizes of pan-genomes. According to extrapolations based on the pan-genomes, core genomes and singletons, the C. pseudotuberculosis biovar ovis shows a more clonal-like behavior than the C. pseudotuberculosis biovar equi. Most of the variable genes of the biovar ovis strains were acquired in a block through horizontal gene transfer and are highly conserved, whereas the biovar equi strains contain great variability, both intra- and inter-biovar, in the 16 detected pathogenicity islands (PAIs). With respect to the gene content of the PAIs, the most interesting finding is the high similarity of the pilus genes in the biovar ovis strains compared with the great variability of these genes in the biovar equi strains. Concluding, the polymerization of complete pilus structures in biovar ovis could be responsible for a remarkable ability of these strains to spread throughout host tissues and penetrate cells to live intracellularly, in contrast with the biovar equi, which rarely attacks visceral organs. Intracellularly, the biovar ovis strains are expected to have less contact with other organisms than the biovar equi strains, thereby explaining the significant clonal-like behavior of the biovar ovis strains.
format	article
author	Siomar C Soares Artur Silva Eva Trost Jochen Blom Rommel Ramos Adriana Carneiro Amjad Ali Anderson R Santos Anne C Pinto Carlos Diniz Eudes G V Barbosa Fernanda A Dorella Flávia Aburjaile Flávia S Rocha Karina K F Nascimento Luís C Guimarães Sintia Almeida Syed S Hassan Syeda M Bakhtiar Ulisses P Pereira Vinicius A C Abreu Maria P C Schneider Anderson Miyoshi Andreas Tauch Vasco Azevedo
author_facet	Siomar C Soares Artur Silva Eva Trost Jochen Blom Rommel Ramos Adriana Carneiro Amjad Ali Anderson R Santos Anne C Pinto Carlos Diniz Eudes G V Barbosa Fernanda A Dorella Flávia Aburjaile Flávia S Rocha Karina K F Nascimento Luís C Guimarães Sintia Almeida Syed S Hassan Syeda M Bakhtiar Ulisses P Pereira Vinicius A C Abreu Maria P C Schneider Anderson Miyoshi Andreas Tauch Vasco Azevedo
author_sort	Siomar C Soares
title	The pan-genome of the animal pathogen Corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains.
title_short	The pan-genome of the animal pathogen Corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains.
title_full	The pan-genome of the animal pathogen Corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains.
title_fullStr	The pan-genome of the animal pathogen Corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains.
title_full_unstemmed	The pan-genome of the animal pathogen Corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains.
title_sort	pan-genome of the animal pathogen corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/81ae3ebdc6ff46e6a121005fed57fb82
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The pan-genome of the animal pathogen Corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains.

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